16通道毛细管阵列电泳对手性氨基酸分离条件的快速筛选

利用自行研制的16通道旋转激光扫描共焦荧光检测式毛细管阵列电泳仪,我们采用了不同浓度的十二烷基硫酸钠、β-环糊精、γ-环糊精对荧光素标记丙氨酸、甲硫氨酸、色氨酸和谷氨酸4种手性氨基酸进行了手性拆分条件研究,快速地获得了这4种手性氨基酸对映体最佳的拆分条件:分离缓冲液为10 mmol/L硼酸缓冲液(pH=10),5 mmol/Lγ-环糊精为添加剂时,4种手性氨基酸全部达到了基线分离。     

Nanostructures formed by combination of nanotube and polymer chain

We have investigated polymeric supramolecular systems of inclusion complexes between molecular nanotube and polymer chains theoretically and experimentally. This system indicates inclusion-dissociation behavior much sharper than the inclusion complex between cyclic molecule and small compounds. And we propose some functional supramolecules utilizing the combination of the nanotube or nanoring and polymer chains such as insulated molecular wire and topological gel.     

氨基酸、多肽的环糊精化学

本文着重介绍了氨基酸、多肽－环糊精连接物的合成,分子识别和自组装,对环糊精及其衍生物与氨基酸、多肽的包合行为,异构体识别和仿酶合成作了简要概述。     

β-环糊精及其衍生物与氮氧自由基作用的ESR研究

Complexation of 4-azido-2,2,6,6-tetramethylpiperidine-1-oxyl (R₁) and 4-oxybenzenesulfonyl-2,2,6,6-tetramethylpiperidine-1-oxyl (R₂) with β-cyclodextrin (β-CD) and [2-O-(2-hydroxypropyl)]-β-cyclodextrin(2-HP-β-CD) in aqueous solution have been studied by electron spin resonance (ESR). The isotropic nitrogen hyperfine coupling constants aN and the rotational correlation time τ_C of the radicals change regularly after com-plexing with β-CD and 2-HP-β-CD. The magnitude of change in τ_C is in order: R₁/2-HP-β-CD >R₁/β-CD; R₁/β-CD >R₂/β-CD, R₁/2-HP-β-CD >R₂/2-HP-β-CD.     

Selective SERS detection of each polycyclic aromatic hydrocarbon (PAH) in a mixture of five kinds of PAHs

This paper reports the qualitative analysis and quantitative detection of polycyclic aromatic hydrocarbon (PAH) molecules with per‐6‐deoxy‐(6‐thio)‐β‐cyclodextrin (CD‐SH) modified gold nanoparticles (AuNPs) by surface‐enhanced Raman scattering (SERS) spectroscopy. For the selective sensing of PAHs, which are environmental pollutants with very low affinity to metallic surfaces, by SERS, a stable substrate with AuNPs and CD‐SH was utilized by supramolecular interaction. Quantitative detection of each PAH was carried out by SERS on inclusion complexes with different concentrations. From the SERS spectra of a mixture of five different PAHs, we could easily distinguish each PAH by its discriminant peaks. In addition, quantitative analysis of one component in a mixture of five PAHs was also investigated. This sensing platform revealed matching relationship between the host and the guest and the host–guest interaction mechanism. The proposed approach for the selective detection of PAHs holds great potential in the detection of environmental organic pollutants. Copyright © 2010 John Wiley & Sons, Ltd.     

New type polyrotaxanes based on polyethyleneimine and α-cyclodextrin

Functional polyrotaxanes of N-alkanoylpolyethyleneimine@ α-cyclodextrin type are synthesized by acylation of the polypseudorotaxane precursor, namely, a polyethylenimine@ α-cyclodextrin inclusion complex, with excess of carboxylic acid anhydrides. In the course of the processing, α-cyclo-dextrin moieties are also fully O-acylated. The introduction of acyl moieties into polyethyleneimine backbone prevents the expulsion of α-cyclodextrin rings from this backbone.     

Polymerization of Styrene in Cyclodextrin Channels: Can Confined Free‐Radical Polymerization Yield Stereoregular Polystyrene?

Summary: Modeling of polystyrenes (PS) with various stereosequences in the narrow cylindrical channels corresponding to those found in γ‐cyclodextrin inclusion compounds (CD ICs) has been conducted. Based on the conformational modeling of stereoisomeric polystyrenes (PSs) in narrow channels, it was suggested that polystyrene with unusual microstructures might be produced by the constrained polymerization of styrene monomer in its γ‐CD‐IC crystals. The in‐situ polymerization of styrene inside the narrow channels of its γ‐CD‐IC crystals was performed in both organic and aqueous media. The ¹³C NMR spectrum of PS synthesized inside the γ‐CD channels in an aqueous medium shows some differences when compared to the ¹³C NMR spectrum of PS synthesized in toluene. These are presumably because of differences in their stereosequences. Here, we report our preliminary findings.

Schematic of cyclodextrin inclusion compound (CD‐IC) channels with included polymer guests.