Molecular Design and Immunogenicity of a Multiple-epitope FMDV Antigen and DNA Vaccination

This article reports the design and construction of a multiple-epitope foot and mouth disease virus （FMDV） antigen, designated as OAAT. This recombinant antigen consists of the structural protein VP1 genes from serotypes A and O FMDV, five major VP1 immunodominant epitopes from two genotypes of Asial serotype, and three Th2 epitopes originating from the nonstructural protein, three ABC gene and structural protein VP4 gene. Expressions of target gene from these plasmids in HeLa cells were verified by Western-blot. BALB/c mice were immunized intramuscularly with the DNA vaccines thrice every two weeks. We found that pA could induce simultaneously specific antibodies against serotypes A, Asial, and O FMDV. Compared to those of the controls, the spots of FMDV-specific IFN-7 and cytotoxic activity from mice immunized with pA were significantly increased, pA provided full protection in 2/4 guinea pigs from challenge with FMDV O/NY00 and Asial/YNBS/58, respectively. The results show that although pA did not give full protection in 100% immunized guinea pigs from challenge with type O and Asial FMDV, respectively, OAAT may be potential immunogen against FMDV and pA may be potential DNA vaccines against FMDV.     

热致相分离法制备食道组织工程用支架的研究

合适的支架是进行组织工程研究必需的条件之一．运用自制的具有较好弹性的无规聚合物聚（L-乳酸-co-己内酯，PLLC），并采用热致相分离法（TIPS）制备了具有不对称孔径的食道组织工程用三维支架．经过对不同成孔条件（包括粗化温度、粗化时间以及聚合物溶液浓度等）的比较和研究，使此支架上表面具有孔径〈10μm，而本体具有50～100μm，甚至〉100gm的不对称多孔支架．并模拟正常食道粘膜层构造，将提取自猪食道的原代上皮细胞和成纤维细胞分别种植于此支架的二面，经HE常规染色，发现在体外培养时间（14d）内，在支架上表面已形成一连续层的上皮细胞层，某些部位甚至多于一层，这与正常食道的上皮细胞层相似．对于成纤维细胞而言，在与上皮细胞层同一视野内所见成纤维细胞较少，因为如果细胞生长在支架内部就很难用常规方法观察得到．但从这些结果可以看到用PLLC制备的多孔支架对细胞没有毒性，且具有一定的支持细胞生长的作用．     

Asymmetric Synthesis of (R)-and (S)-Moprolol

A simple and effective procedure for the enantioselective synthesis of (R)-and (S)-moprolol was described.The key step was the asymmetric synthesis of enantiopure (R)-and (S)-guaifenesin,which were synthesized from enantioenriched (R)-3-chloro-1,2-propanediol and (S)-epichlorohydrin via kinetics of hydrolysis resolution of racemic epichlorohydrin by chiral Salen-CoIIII complex.The e.e.values of both the optical compounds were above 98%,and the chemical structures of the target compounds were confirmed by 1H NMR,13C NMR,IR,and MS.     

SYNTHESIS AND BIOLOGICAL ACTIVITY OF A NEW FROG SKIN PEPTIDE, RANAMARGARIN

A new frog skin peptide, ranamargarin depicted as H-Asp-Asp-Ala-Ser-Asp-Arg-Ala-Lys-Lys-Phe-Tyr-Gly-Leu-Met-NH_(2′), was synthesized by the conventionalmethod. Comparisons of chemical and biological properties of both the synthetic and natural ranamargarins indicated that they were identical, so the chemical structure of ranamargarin was confirmed. Preliminary pharmacological study showed that ranamargarin was highly selective towards the SP-P subtype receptor.     

浅杂质势与窄量子阱的耦合作用

本文采用一种新的变分波函数描述GaAs/Ga_1-xAl_xAs窄量子阱中的浅施主基态,并计算了杂质基态波函数和结合能。计算所得数值结果表明正确考虑窄量子阱与杂质势间的耦合作用是极为重要的。 关键词：     

从相关测量的数据恢复超短激光脉冲的波形

本文证明了,只要测量了脉冲光强的二阶相关函数G~(2)(τ)和单延迟的三阶相关函数G~(3)(τ),便可以恢复超短激光脉冲的强度波形。并提出一种恢复脉冲波形的计算方法,编制了计算机程序,对实验数据作了处理,证实这种计算方法是可行的。在此基础上建议一种通过相关测量确定超短激光脉冲波形的新型仪器。     

等离子体诊断中的Abel逆变换

本文描述了两种最常用的Abel逆变换方法,并用这两种方法对不同类型的分布曲线在计算机上进行了模拟计算;研究了输入数据的随机误差、诊断设备的系统误差以及采样点的数目等对Ab-el逆变换精度的影响,同时对这两种方法进行了比较。     

非素数阶幻方的构造

基于矩阵运算,给出任意双偶数阶和非素数阶幻方的新构造方法:1)由任一低阶m(m为偶数且m≠2)幻方生成一高阶2m阶幻方;2)利用已知的m(m≠2)阶和n(n≠2)阶两个幻方,构造任意的非素数mn阶幻方,加强一些条件后,进一步提出构造两类高级幻方(泛对角线幻方和关联幻方)的新方法.     

基于粗糙集的经济预警规则发现

应用粗糙集的理论和方法对经济预警有关数据进行分析,挖掘其中隐含的有用信息,提取规则并对规则进行约简,从而求取表达经济预警信息的最小决策规则,为经济预警有用信息的获取提供一种有效的方法.