HFP—2500F高频发生器故障诊断与维修

本文介绍了高频发生器在日常工作中经常出现的故障，以及诊断排除方法。     

小菜蛾颗粒体病毒DNA限制性酶切分析及基因组DNA片段克隆

小菜蛾颗粒体病毒ＤＮＡ用ＢａｍＨＩ和ＸｈｏＩ酶切，经０．７５％琼脂糖凝胶电泳，分别得到１２条带和８条带，平均分子量为１１３．０ｋｂ．用Ｓｕｐｅｒｃｏｓ１Ｃｏｓｍｉｄ作载体，将Ｓａｕ３ＡＩ部分消化的ＰｘＧＶ－ＤＮＡ随机片段插入该载体的ＢａｍＨＩ酶切位点，转化于ＸＬ１－Ｂｌｕｅ受体菌，经Ａｍｐ平板筛选转化子，挑出２５６个Ａｍｐ＋菌落，以３２Ｐ－ｄＣＴＰ标记的ＰｘＧＶ－ＤＮＡ为探针，斑点杂交筛选得到５９个阳性重组体，再经电泳检测，得到了５９个不同大小的ＰｘＧＶ－ＤＮＡ片段克隆．     

Porous metal absorbers for underwater sound

Rubber has traditionally been used for underwater sound absorption. Porous metal is a relatively lightweight material and also has higher strength than rubber. However, exactly how porous metals can be used as effective underwater sound absorbers remains unclear. This paper shows how to use porous metal absorbers so that they work well under water, even under fairly constrained conditions. A method of nondimensional analysis is proposed that allows identification of vital characteristics. This means that such characteristics can be varied and the absorbers themselves filled with different types of viscous fluids. Such analysis suggests that the sound absorption coefficient of porous metals does not always increase when there are either increases in porosity or decreases in average pore size. The same method of analysis can show how, by choice of the right characteristics to choose a suitable viscous fluid, a porous metal absorber can be built that takes up little space but still effectively absorbs underwater sounds at low frequencies.     

陶瓷阻力曲线测定的新方法——断裂栅法

将零厚度单向试件栅刻蚀工艺应用于晶须增韧氮化硅陶瓷基复合材料表面，通过记录试件所在桥路的输出电压值，可得到随载荷不断增大裂纹逐渐向前扩展过程中每一时刻的裂尖位置和裂纹长度，从而得到该种材料的阻力曲线。     

Solution of elastic-plastic shallow shell problems by the boundary element method

The boundary integral equations for elasto-plastic problems of shallow shells are established by using the fundamental solutions of shallow shells derived previously. The strains and stress-resultants in the plastic region are used as unknown variables. The simultaneous nonlinear equations of these variables and unknown boundary values are established and solved by direct iteration method.     

Phenylmethanesulfonyl fluoride pretreatment stabilizes plasma lipidome in lipidomic and metabolomic analysis

Though it is standard practice to test the stability of analytes in the matrix for routine bioanalytical method, stability evaluation is always impractical and skipped in untargeted lipidomic and metabolomic analysis because analytes in these studies are enormous, diverse and sometimes unknown. Lipidome represents a major class of plasma metabolome and shows great potential to be diagnostic and prognostic biomarkers. However, lipidome also faces stability problems because plasma contains kinds of lipid degradation enzyme. Here, using liquid chromatography time of flight mass spectrometry based lipidomic methodology, plasma levels of various lipids including triglyceride (TG), diglyceride (DG), free fatty acid (FFA), phosphatidylethanolamine (PE) phosphatidylcholine (PC), lyso-phosphatidylcholine (LPC), lyso-phosphatidylethanolamine (LPE), and sphingomyelin (SM) were dynamically determined within 4 h at ambient temperature. In mouse and rat plasma, the levels of most TG, DG, PC and PE species significantly decreased with respect to time, whereas those of LPC, LPE and FFA significantly increased with respect to time. However, such changes did not occur in human plasma, thus indicating hepatic lipase and esterase might involve in the species-specified degradation of lipid classes in plasma. Phenylmethanesulfonyl fluoride (PMSF) pretreatment prevented such lipidome instability in mouse plasma. The results suggested the instability of plasma lipidome should be highly concerned, and the enhancement of ex vivo stability of plasma lipidome could enable more reliable clinical translation of lipidomic data for biomarker discovery.     

A NEW METHOD TO DETERMINE THE R-CURVE OF CERAMICS-FRACTURE GRATING METHOD

The etching technique of the single-lined zero-thickness specimen grating is applied tothe surface of the SiC fiber toughening Si_3N_4 ceramic composite specimen.The position of the crackand the crack length during the process of crack extension when the load is applied and gradually in-creased can be determined by recording the output voltage value of the Wheatstone bridge in which theceramic specimen with the fracture grating on is located.The crack-growth-resistance(R-curve) of thismaterialis thus obtained.     

Delay-independent exponential stability of stochastic Cohen–Grossberg neural networks with time-varying delays and reaction–diffusion terms

Different from the approaches used in the earlier papers, in this paper, the Halanay inequality technique, in combination with the Lyapunov method, is exploited to establish a delay-independent sufficient condition for the exponential stability of stochastic Cohen–Grossberg neural networks with time-varying delays and reaction–diffusion terms. Moreover, for the deterministic delayed Cohen–Grossberg neural networks, with or without reaction–diffusion terms, sufficient criteria for their global exponential stability are also obtained. The proposed results improve and extend those in the earlier literature and are easier to verify. An example is also given to illustrate the correctness of our results.     

Li_4SiO_4陶瓷小球水解吸行为实验研究

采用程序升温热解吸(TPD/TDS)方法对Li4SiO4陶瓷小球的水解吸行为进行了实验研究。结果表明:水解吸过程中主要存在四个解吸峰;其中100℃附近的峰可解释为物理吸附水;150,250,400℃附近的峰可分别解释为以氢键、Li-OH和Si-OH配位键形式存在的化学吸附水。氚的释放与水的解吸几乎同步进行,且氚的释放形式主要为氚水(HTO),据此推测,氚水可能存在三种释放机制:(1)-OT+H2O→-OH+HTO;(2)-OH+-OH→H2O,-OT+H2O→-OH+HTO;(3)-OT+-OH→HTO。