Finite element analysis of broadband acoustic pulses through inhomogenous media with power law attenuation

Acoustic waves in tissues and weakly attenuative fluids often have an attenuation parameter, alpha(omega), satisfying alpha(omega)= alpha0omegay in which alpha0 is a constant, omega is the frequency, and y is between 1 and 2. This power law attenuation is not predicted by the classical thermoviscous wave equation and researchers have proposed different modified viscous wave equations in which the loss term is a convolution operator or a fractional spatial or temporal derivative. In this paper, acoustic waves undergoing power law attenuation are modeled by a modification to the thermoviscous wave equation in which the time derivative of the viscous term is replaced by a fractional time derivative. An explicit time domain, finite element formulation leads to a stable algorithm capable of simulating axisymmetric, broadband acoustic pulses propagating through attenuative and dispersive media. The algorithm does not depend on the Born approximation, long wavelength limit, or plane wave assumptions. The algorithm is validated for planar and focused transducers and results include radiation patterns from a viscous scatterer in a lossless background and signals reflected from a viscous layer. The program can be used to determine scattering parameters for large, strong, possibly viscous scatterers, in either a lossless or viscous background, for which analytic results are scarce.     

Distortion in the similitude of soil-machine systems

This paper contains an appraisal of the current knowledge concerning sources of distortion in similitude studies and techniques used to deal with it. The general philosophy and basic principles that relate the concepts of similitude and studies of soil-machine systems have been discussed in detail in the paper, Similitude Studies of Soil-Machine Systems by Freitag et al. Some of that material is repeated to help define the condition referred to as distortion and to establish the effects of distorted models in the application of similitude to the analysis of soil-machine systems.     

Suppression of plasma turbulence during optimized shear configurations in JET

Correlation of density turbulence suppression and reduced plasma transport is observed in the internal transport barrier (ITB) region of JET tokamak discharges with optimized magnetic shear. The suppression occurs in two stages. First, low frequency turbulence and ion transport are reduced across the plasma core by a toroidal velocity shear generated by intense auxiliary heating. Then with the ITB formation, high frequency turbulence and electron transport are reduced locally within the steep pressure gradient region of the ITB.     

Mass Activated Droplet Sorting (MADS) Enables High-Throughput Screening of Enzymatic Reactions at Nanoliter Scale

Microfluidic droplet sorting enables the high-throughput screening and selection of water-in-oil microreactors at speeds and volumes unparalleled by traditional well-plate approaches. Most such systems sort using fluorescent reporters on modified substrates or reactions that are rarely industrially relevant. We describe a microfluidic system for high-throughput sorting of nanoliter droplets based on direct detection using electrospray ionization mass spectrometry (ESI-MS). Droplets are split, one portion is analyzed by ESI-MS, and the second portion is sorted based on the MS result. Throughput of 0.7 samples s⁻¹ is achieved with 98 % accuracy using a self-correcting and adaptive sorting algorithm. We use the system to screen ≈15 000 samples in 6 h and demonstrate its utility by sorting 25 nL droplets containing transaminase expressed in vitro. Label-free ESI-MS droplet screening expands the toolbox for droplet detection and recovery, improving the applicability of droplet sorting to protein engineering, drug discovery, and diagnostic workflows.     

Photon Equivalents as a Parameter for Scaling Photoredox Reactions in Flow: Translation of Photocatalytic C−N Cross-Coupling from Lab Scale to Multikilogram Scale

With the development of new photocatalytic methods over recent decades, the translation of these chemical reactions to industrial-production scales using continuous-flow reactors has become a topic of increasing interest. In this context, we describe our studies toward elucidating an empirically derived parameter for scaling photocatalytic reactions in flow. By evaluating the performance of a photocatalytic C−N cross-coupling reaction across multiple reactor sizes and geometries, it was demonstrated that expressing product yield as a function of the absorbed photon equivalents provides a predictive, empirical scaling parameter. Through the use of this scaling factor and characterization of the photonic flux within each reactor, the cross-coupling was scaled successfully from the milligram scale in batch to a multi-kilogram reaction in flow.     

Integrated system modeling and optimization via quasilinearization

System modeling and system optimization are two coupled and strongly related concepts in the modern approach to large-scale systems. Yet, they have been treated as two separate problems in the literature. The identification of system parameters, often referred to as system modeling, is essential in order to obtain an optimal control policy. This work considers the two problems jointly and provides a computational methodology in tackling the integrated problem formulation. This is done by viewing one of the objective functions in the bicriterion problem formulation as a constraint. A computational strategy such as quasilinearization is employed for the solution of the integrated problem. An example problem is introduced, and numerical results using an IBM 360/91 digital computer are presented.The authors are very grateful to Professor C. T. Leondes for his invaluable assistance, guidance, and comments. This research was supported in part by the Air Force Office of Scientific Research, Grant No. 699-67, and in part by the National Science Foundation, Grant No. GK-4086.     

Metal-sensitive and thermostable trypsin from the crevalle jack (<Emphasis Type="Italic">Caranx hippos</Emphasis>) pyloric caeca: purification and characterization

Background

Over the past decades, the economic development and world population growth has led to increased for food demand. Increasing the fish production is considered one of the alternatives to meet the increased food demand, but the processing of fish leads to by-products such as skin, bones and viscera, a source of environmental contamination. Fish viscera have been reported as an important source of digestive proteases with interesting characteristics for biotechnological processes. Thus, the aim of this study was to purify and to characterize a trypsin from the processing by-products of crevalle jack (Caranx hippos) fish.

Results

A 27.5 kDa trypsin with N-terminal amino acid sequence IVGGFECTPHVFAYQ was easily purified from the pyloric caeca of the crevalle jack. Its physicochemical and kinetic properties were evaluated using N-α-benzoyl-_DL-arginine-p-nitroanilide (BApNA) as substrate. In addition, the effects of various metal ions and specific protease inhibitors on trypsin activity were determined. Optimum pH and temperature were 8.0 and 50°C, respectively. After incubation at 50°C for 30 min the enzyme lost only 20% of its activity. K _m , k_cat, and k _cat /K _m values using BApNA as substrate were 0.689 mM, 6.9 s^-1, and 10 s^-1 mM^-1, respectively. High inhibition of trypsin activity was observed after incubation with Cd²⁺, Al³⁺, Zn²⁺, Cu²⁺, Pb²⁺, and Hg²⁺ at 1 mM, revealing high sensitivity of the enzyme to metal ions.

Conclusions

Extraction of a thermostable trypsin from by-products of the fishery industry confirms the potential of these materials as an alternative source of these biomolecules. Furthermore, the results suggest that this trypsin-like enzyme presents interesting biotechnological properties for industrial applications.

     

Nuclear orbiting and anomalies in nuclear reactions

In this paper, we report our measurements of back-angle oxygen and carbon particle yields from ¹⁶O+⁸⁹Y, ¹²C+⁹³Nb reactions forming the same compound nucleus ¹⁰⁵Ag at the same excitation energy and spin distribution. We find anomalously large oxygen yield and entrance channel dependence at high excitation energies from ¹⁶O+⁸⁹Y reaction implying formation of a dinuclear orbiting complex. Possible connection between nuclear orbiting and fast fission is also discussed.