A New Compound Isolated from a Bioactive Streptomycete

A new compound, named (S)-α-acetylamino-β-(3-indole)propano1 (1) was isolated, along with two known compounds teleocidin B 1 and emycin B from culture broth of a streptomycete strain YIM33176 (streptomyces pleomorphus). The structure of 1 was determined by detailed spectroscopic investigation.     

A New Flavone Glycoside from Isodon enanderianus

Isodon enanderianus (Hand.-Mazz.) H. W. Li, a perennial shrub plant of Labiatae family, is widely distributed in the southern part of Yunnan province. It has long been used as folk medicine to diminish inflammation and detoxify1. The Isodon genus is known to be rich in ent-kaurane diterpenoids, a series of new ent-kaurane diterpenoids have been isolated from the dried leaves of I. enanderianus2-4. During the course on a re-investigation of the chemical constituents of I. enanderianus, four…     

A New Benzosesquiterpenoid Dimer from Polyalthia cheliensis Hu

The genus plants Polyalthia, being rich in bioactive diterpenoids and alkaloids1,2, have been attracting much attention. Polyalthia cheliensis Hu is a bush mainly distributed in Xishuangbanna, Yunnan Province of China. The previous researches1 reported the isolations of four clerodane diterpenes from the stem bark of this plants. Recent phytochemical work on its dried leaves results in the elucidations of a new benzosesquiterpenoid dimer, 3((, 3(((-bispolycerasoidol (1) and its monomer, a kn…     

A New Diterpene Glycoside from Isodon forrestii

The genus Isodon has been shown to be a rich source of diterpenes, especially the highly oxidized ent-kaurene diterpenoids with various biological activities1-3. The chemical constituents of Isodon forrestii was reported by Yun Long Xu et al.4. We have investigated the titled plant collected in another place, from the 70% acetone extract of the aerial parts of I. forrestii, seven compounds including a new diterpene glycoside 1 were isolated by a combination of silica gel, preparative TLC an…     

A Novel Hexanordammarane Glycoside from the Roots of Panax notoginseng

There are extensive chemical studies on the roots of Panax notoginseng (Burk.) F. H. Chen, a famous traditional Chinese medicine called San-Qi or Tian-Qi, thirty-three dammarane saponins were isolated and their structures were identified by our group and other scientists1-8. Further chemical investigation on this plant led to the isolation of a novel hexanordammarane glycoside, named notoginsenoside R10 (1).Notoginsenoside R10 (1), white powder, negative HRFAB-MS showed a quasimolecular…     

A New Abietane Diterpenoid from the Barks of Taxus yunnanensis

Along with the major constituents taxoids, some non-taxane diterpenoids have also been isolated1. During the course of our phytochemical investigation on the bark of Taxus yunnanensis. Cheng et L. K. Fu, a new abietane diterpenoid, taxayunnin (1), and the known taxamairin C (2)2, were isolated. The structure elucidation of taxayunnin 1 was established by spectral analysis.Taxayunnin 1, pale yellow needles, mp 215oC, showed a molecular ion peak at m/z 346 [M+] in its EI mass spectrum, which…     

Leiocyclocin A and B, Two Cyclopeptides from Goniothalamus leiocarpus

Ongoing phytochemical studies on Goniothalamus species have led to the isolation of a number of annonaceous acetogenins and unusual new styryllactones which were found to possess significant cytotoxic activities against several hunan tumour cell lines1,2. Firstly, cyclopeptides were reported to be isolated from genus Goniothalamus in this paper.Goniothalamus leiocarpus is an evergreen tree which grows in the south of Yunnan, China. Four new anticancer styryllactones3,4 from the stem bark and…     

Three New Saikosaponin-like Compounds from Polycarpon prostratum

In our previous paper~（1-4）, we have reported five new tetraglycosides (named prostratoside A-E) and a new cyclic peptide (named polycarponin A) from this plant. Our continuing study on the EtOAc soluble fraction of this plant led to the isolation of three new saikosaponin-like compounds: prostratoside F-H (1-3). This paper describes the structure elucidation of these three new compounds. Prostratosid F (1): white powder, mp. 186-188oC, [(]+22.0 (c 0.27, MeOH). Its HRFAB-MS gave a […     

液相色谱-串联质谱法测定吉非替尼中4种基因毒性杂质

建立了一种同时测定吉非替尼中4种基因毒性杂质3-氯-4-氟苯胺、3,4-二氟苯胺、3-氟-4-氯苯胺和3,4-二氯苯胺的高效液相色谱-串联质谱（HPLC-MS/MS）方法。用Inertsil ODS-3柱（100 mm×3.0 mm,3μm）为色谱柱,以0.1%（体积分数,下同）甲酸水溶液-0.1%甲酸乙腈溶液为流动相,在电喷雾正离子模式下进行测定。该方法在特异性、线性、精密度、准确性、稳定性和耐用性方面得到了验证。4种基因毒性杂质在0.6~96.0μg/L范围内与峰面积呈良好线性关系。检测限和定量限分别为0.2~2.0μg/L和0.6~6.0μg/L。所有杂质的回收率为91.0%~98.5%。检测后,在批号16052301和R16052501-1样品中仅检测到3-氯-4-氟苯胺,但低于杂质限度（6 mg/L）。该方法简便可靠,可用于吉非替尼中4种基因毒性杂质的测定,并为质量控制提供参考。     

百合知母汤的高效液相色谱指纹图谱及其与组方药味的相关性

研究和建立了百合知母汤的高效液相色谱(HPLC)指纹图谱,为研究百合知母汤的药效物质基础及配伍变化提供了手段。采用Agela Venusil XBP-C18色谱柱(250 mm × 4.6 mm,5 μm),以乙腈和0.1%甲酸为流动相二元梯度洗脱,流速1 mL/min,检测波长315 nm,柱温25 ℃。以芒果苷为参照物,在相同的色谱条件下测定了10批不同产地的百合与知母制备的百合知母汤的指纹图谱,获得了16个共有指纹峰,通过与对照品的保留时间及紫外光谱比较,标定了5-羟甲基糠醛(5-HMF)、新芒果苷、芒果苷、异芒果苷、王百合苷B的出峰位置。该方法得到的百合知母汤的指纹图谱特征性和重现性较好,方法稳定、可靠,可以为百合知母汤的质量控制提供参考。通过实验归属了百合知母汤指纹图谱中的主要色谱峰,并确定了煎煮过程中的主要变化成分为5-HMF。