Preparation and Characterization of Three-dimensionally Ordered Crystalline Macroporous CeO2

Three-dimensional ordered macroporous (3DOM) materials are of great potential importance in catalysis, selective separation, sensor arrays and communications with optical band gaps1. Many results in the synthesis of 3DOM materials have been reported, such as closed-packed silica spheres or polystyrenes (PS) templating routes for silica2, metal oxides1-6 and metals7. Recently, poly(methyl methacrylate) (PMMA) colloidal crystal templates have been used to prepare 3DOM materials of metals8,…     

Preparation of Diphenyl Oxalate from Transesterification of Dimethyl Oxalate with Phenol over TS—1 Catalyst

Diphenyl oxalate was synthesized from transesterification of dimethyl oxalate with phenol over TS-1(2.5 wt% Ti)catalyst.TS-1 catalyst,as a beterogeneous catalyst,showed excellent selectivity of diphenyl oxalate and methylphenyl oxalate compared with other homogeneous catalysts.Lewis acid sites on TS-1 catalyst were the active sites for transesterification of dimethyl oxalate with phenol.the high selectivity was closely related to the weak acid sites over TS-1.     

N,N,N′′-三取代氨基甲酰脲类化合物的合成

N，N－二取代脲和取代三氯乙酰胺反应合成了10种未见文献报道的标题化合物，产物结构经元素分析、IR和^1H NMR确证。初步生物活性测定表明多数化合物具有明显的除草活性。     

Gene expression analysis for high throughput screening applications

To meet growing needs for high throughput gene expression profiling, we established a new automated high throughput TaqMan RT-PCR method for quantitative mRNA expression analysis. In this method, the Allegro( trade mark ) (Zymark) system conducts all sample tracking and liquid handling steps, and ABI PRISM 7900 HT (Applied Biosystems) is used to conduct real-time determination of the C(t) value when amplification of PCR products is first detected and accumulation of inhibitory PCR products is unlikely to occur. The ABI PRISM 7900 HT Sequence Detection System features a real-time PCR instrument with 384-well-plate compatibility and robotic loading, and continuous wavelength detection, which enables the use of multiple fluorophores in a single reaction. The Allegro System offers an assembly line approach with a modular design that allows reconfiguration of the components to accommodate variations in the assay flow. In the present study, we have established and validated a new automated High Throughput (HT) TaqMan RT-PCR- based method for quantitative mRNA expression analysis. The data demonstrate that HT-Taqman PCR is a powerful tool that can be used for measuring low concentrations of mRNA, and is highly accurate, reproducible, and amenable to high throughput analysis. Results suggest that HT-TaqMan is a reliable method for the quantification of low-expression genes and a powerful tool with HT capability for target identification/validation, structure-activity relationship (SAR) study, compound selection for efficacy studies, and biomarker identification in drug discovery and development.     

二(芳氧基)稀土(Ⅱ)配合物催化ε-己内酯开环聚合

系统地研究了二（２，６ 二叔丁基 ４ 甲基酚基）钐［（ＡｒＯ）２Ｓｍ（ＴＨＦ）４］催化ε 己内酯的开环聚合，发现它具有很高的催化活性并显示“活性”聚合的特点，在甲苯中，当［Ｍ］／［Ｉ］＝２０００（摩尔比），６０℃，１ｈ，转化率可达９８％．并比较了不同的两价稀土化合物的催化活性．通过核磁分析末端基结构的方法，研究了（ＡｒＯ）２Ｓｍ催化己内酯开环聚合的引发机理，发现催化剂首先与己内酯反应，生成三价烯醇式稀土化合物，后者引发己内酯聚合．     

Love波免疫传感器在免疫分析中的研究

Love波免疫传感器是一种以免疫反应为识别方式的新型声表面波传感器。与其它压电声波免疫传感技术相比,水平剪切型表面波、高的压电晶体基频以及声波导层的存在使Love波免疫传感器在可用于气液两相检测的同时具有更高的灵敏度,从而可以成为免疫分析中的一种重要的工具。本文分别从Love波传感器的构造、原理、发展现状和以抗体作为探针在传感器表面的固定化方法两方面综述了Love波免疫传感器的研究进展。     

酞菁敏化混晶TiO2薄膜的低温制备及其对罗丹明B的催化降解

以四磺酸酞菁铜(CuPcTs)为敏化剂,采用溶胶-凝胶法制备了酞菁敏化的TiO2溶胶,经浸渍提拉成膜并室温晾干,制得CuPcTs-TiO2薄膜.采用XRD,UV-Vis和SEM等测试手段对薄膜样品进行了表征,并通过罗丹明B的降解考察了薄膜的可见光催化性能.结果表明,TiO2为锐钛矿-板钛矿-金红石混晶结构,CuPcTs-TiO2薄膜对罗丹明B降解具有较高的光催化活性.CuPcTs敏化能有效提高TiO2的可见光催化活性,但过多的CuPcTs会使催化活性降低.在可见光照射条件下,罗丹明B主要是先脱乙基生成罗丹明,然后再逐步被分解.CuPcTs-TiO2薄膜在经2～3次循环使用后,其光催化活性趋于稳定.CuPcTs可牢固地负载于TiO2薄膜中,从而保证了CuPcTs-TiO2薄膜重复使用的稳定性.     

A new cucurbitane triterpenoid from Momordica charantia

One new cucurbitane-type triterpenoid saponin, 5b,19-epoxycucurbita-6,23-diene-3b,19,25-triol-3-O-b-D-allopyranoside (1), named momordicoside P was isolated from the fresh fruits of Momordica charantia. The structure of the saponin was elucidated byspectral methods, including 2D-NMR spectra.     

Synthesis and Structure of 2‐Substituted Thieno[3′,2′:5,6]pyrido[4,3‐d]pyrimidin‐4(3H)‐one Derivatives

A series of new 2‐substituted 3‐(4‐chlorophenyl)‐5,8,9‐trimethylthieno[3′,2′: 5,6]pyrido[4,3‐d]pyrimidin‐4(3H)‐ones 8 were synthesized via an aza‐Wittig reaction. Phosphoranylideneamino derivatives 6a or 6b reacted with 4‐chlorophenyl isocyanate to give carbodiimide derivatives 7a or 7b , respectively, which were further treated with amines or phenols to give compounds 8 in the presence of a catalytic amount of EtONa or K₂CO₃. The structure of 2‐(4‐chlorophenoxy)‐3‐(4‐chlorophenyl)‐5,8,9‐trimethylthieno[3′,2′: 5,6]pyrido[4,3‐d]pyrimidin‐4(3H)‐one ( 8j ) was comfirmed by X‐ray analysis.