Evidence for an elongated (>60 ion skin depths) electron diffusion region during fast magnetic reconnection

Observations of an extremely elongated electron diffusion region occurring during fast reconnection are presented. Cluster spacecraft in situ observations of an expanding reconnection exhaust reveal a broad current layer ( approximately 10 ion skin depths thick) supporting the reversal of the reconnecting magnetic field together with an intense current embedded at the center that is due to a super-Alfvénic electron outflow jet with transverse scale of approximately 9 electron skin depths. The electron jet extends at least 60 ion skin depths downstream from the X-line.     

Stability of a stationary steam-water front in a porous medium

The instability of a plane front between two phases of the same fluid (steam and water) in a porous medium is considered. The configuration is taken to be initially stationary with the more dense phase overlying the less dense phase. The frontal region is assumed sharp, so that macroscopic boundary conditions can be utilized. This assumption precludes the existence of dispersion instabilities. The stabilizing influence of phrase transition as well as the implication of different macroscopic pressure boundary conditions on the stability of the front are discussed and illustrated.     

Steady-state countercurrent flow in one dimension

Two phase countercurrent steady-state flow through permeable media in one dimension is discussed. For steady-state countercurrent flow in water wet porous media, a saturation profile is predicted with the water saturation decreasing in the direction that the water phase is flowing. The de la Cruz and Spanos equations predict that the Muskat relative permeability curves for countercurrent flow will be less than the Muskat relative permeability curves for steady-state cocurrent flow. This result has immediate implications regarding the use of external drive techniques to determine relative permeabilities based on the Buckley-Leverett theory and Muskat's equations. These equations and current experimental evidence involving countercurrent flow indicate that Muskat's equations do not adequately describe the multiphase flow of immiscible fluids.     

Wavelength-dependent enhancement of DCP metal lines by saline matrices

Sodium-induced emission enhancement of transition metal resonance lines are measured in a d.c. plasma (DCP) for wavelengths from 210 to 395 nm. Systematic differences in enhancement are observed within individual spectra (Fe I, Ni I, Sc II), and the enhancement and the excitation potential of a line are found to be linearly related. Electron density and apparent temperature data lead to an interpretation of thiis energy dependence within the context of a recombining plasma in partial thermodynamic equilibrium.     

How does a drug molecule find its target binding site?

Although the thermodynamic principles that control the binding of drug molecules to their protein targets are well understood, detailed experimental characterization of the process by which such binding occurs has proven challenging. We conducted relatively long, unguided molecular dynamics simulations in which a ligand (the cancer drug dasatinib or the kinase inhibitor PP1) was initially placed at a random location within a box that also contained a protein (Src kinase) to which that ligand was known to bind. In several of these simulations, the ligand correctly identified its target binding site, forming a complex virtually identical to the crystallographically determined bound structure. The simulated trajectories provide a continuous, atomic-level view of the entire binding process, revealing persistent and noteworthy intermediate conformations and shedding light on the role of water molecules. The technique we employed, which does not assume any prior knowledge of the binding site's location, may prove particularly useful in the development of allosteric inhibitors that target previously undiscovered binding sites.     

An enzyme-initiated Smiles rearrangement enables the development of an assay of MshB, the GlcNAc-Ins deacetylase of mycothiol biosynthesis

MshB is the N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-D-glucopyranoside (GlcNAc-Ins) deacetylase active as one of the enzymes involved in the biosynthesis of mycothiol (MSH), a protective low molecular weight thiol present only in Mycobacterium tuberculosis and other actinomycetes. In this study, structural analogues of GlcNAc-Ins in which the inosityl moiety is replaced by a chromophore were synthesized and evaluated as alternate substrates of MshB, with the goal of identifying a compound that would be useful in high-throughput assays of the enzyme. In an unexpected and surprising finding one of the GlcNAc-Ins analogues is shown to undergo a Smiles rearrangement upon MshB-mediated deacetylation, uncovering a free thiol group. We demonstrate that this chemistry can be exploited for the development of the first continuous assay of MshB activity based on the detection of thiol formation by DTNB (Ellman's reagent); such an assay should be ideally suited for the identification of MshB inhibitors by means of high-throughput screens in microplates.