排序方式: 共有78条查询结果,搜索用时 31 毫秒
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Mechanically stable porous cellulose media for affinity purification of family 9 cellulose-binding module-tagged fusion proteins 总被引:1,自引:0,他引:1
A mechanically stable cellulose-based chromatography media was synthesized to permit inexpensive affinity purification of recombinant proteins containing the family 9 carbohydrate-binding module (CBM9) fused to either the N- or C-terminus of the target protein. A second-order response surface model was used to identify optimal concentrations of the primary reactants, epichlorohydrin and dimethyl sulfoxide (DMSO), required to cross-link the starting material, Perloza MT100, a compressible but inexpensive cellulose-based chromatography resin. This resulted in a mechanically stable cross-linked affinity chromatography media capable of operating at an order-of-magnitude higher linear velocity than permitted by unmodified MT100. Moments and Van Deemter analyses were used to show that rates of solute mass transfer within the column are largely unaffected by the cross-linking reaction, while the binding capacity decreased by 20% to 7.1 micromol of protein/g resin, a value superior to most commercial affinity chromatography media. In sharp contrast to MT100, the mechanical stability and purification performance of the cross-linked media are not diminished by scale-up or repeated column use. 相似文献
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Mortezaei Zohreh Zendehdel Mojgan Bodaghifard Mohammad Ali 《Research on Chemical Intermediates》2020,46(4):2169-2193
Research on Chemical Intermediates - In this work, magnetic nanocomposite of NaP Zeolite and CoFe2O4 as magnetic nanoparticles (MNP’s) with different ratios were prepared and in the second... 相似文献
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Jacqkis Davis Mojgan Gharaee Dr. Chamini V. Karunaratne Jose Cortes Vazquez Mikayla Haynes Prof. Weiwei Luo Dr. Vladimir N. Nesterov Prof. Thomas Cundari Prof. Hong Wang 《Chemistry (Weinheim an der Bergstrasse, Germany)》2022,28(27):e202200224
Cooperative enamine-metal Lewis acid catalysis has emerged as a powerful tool to construct carbon-carbon and carbon-heteroatom bond forming reactions. A concise synthetic method for asymmetric synthesis of chromans from cyclohexanones and salicylaldehydes has been developed to afford tricyclic chromans containing three consecutive stereogenic centers in good yields (up to 87 %) and stereoselectivity (up to 99 % ee and 11 : 1 : 1 dr). This difficult organic transformation was achieved through bifunctional enamine-metal Lewis acid catalysis. It is believed that the strong activation of the salicylaldehydes through chelating to the metal Lewis acid and the bifunctional nature of the catalyst accounts for the high yields and enantioselectivity of the reaction. The absolute configurations of the chroman products were established through X-ray crystallography. DFT calculations were conducted to understand the mechanism and stereoselectivity of this reaction. 相似文献
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Mojgan Ghabarpoor Majid Tavassoli Kajani Masoud Allame 《Mathematical Methods in the Applied Sciences》2019,42(5):1609-1621
This paper presents a new multistep collocation method for nth order differential equations. The interval of interest is first divided into N subintervals. By determining interval conditions in Taylor interpolation in each subinterval, Taylor polynomials are calculated with different step lengths. Then the obtained solutions in each subinterval are used as initial conditions in the next step. Optimal error is assessed using Peano lemma, which shows that the errors decay by decreasing the step length. In particular, for fixed step length h, the error is of O(m?m), where m is the number of collocation points in each subinterval. Meanwhile, for fixed m, the error is of O(hm). Numerical examples demonstrate the validity and high accuracy of the proposed method even for stiff problems. 相似文献
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Prostanoids are potent mediators of many physiological and pathophysiological processes. Of the many analytical methodologies used for their qualitative and quantitative analysis, electrospray tandem mass spectrometry coupled to liquid chromatography (LC/ESI-MS/MS) offers a rapid, sensitive and versatile system applicable to lipidomic analyses. We have developed an LC/ESI-MS/MS assay for twenty-seven mediators including prostaglandins, prostacyclines, thromboxanes, dihydroprostaglandins and isoprostanes. The assay was liner over the concentration range 1-100 pg/microL. The limits of detection and quantitation were 0.5-50 and 2-100 pg, respectively, whilst recoveries were from 83-116% depending on the metabolite. The assay can be applied to the profiling of prostanoids produced by a variety of biological fluids and extracts including brain, liver, plasma and urine, thus facilitating our understanding of the role of these lipid mediators in health and disease, as well as assisting in drug development. 相似文献
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Universality of Coproducts in Categories of Lax Algebras 总被引:1,自引:1,他引:0
Categories of lax -algebras are shown to have pullback-stable coproducts if preserves inverse images. The general result not only gives a common proof of this property in many topological categories but also shows that important topological categories, like the category of uniform spaces, are not presentable as a category of lax -algebras, with preserving inverse images. Moreover, we show that any such category of -algebras has a concrete, coproduct preserving functor into the category of topological spaces. 相似文献
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Mojgan Mahmoudi Halimeh Moghbeli Konrad Pióro 《Journal of Pure and Applied Algebra》2019,223(10):4161-4170
The study of algebraic properties of ordered structures has shown that their behavior in many cases is different from algebraic structures. For example, the analogues of the fundamental mapping theorem for sets which characterizes surjective maps as quotient sets modulo their kernel relations, is not true for order-preserving maps between posets (partially ordered sets). The main objective of this paper is to study the quotients of dcpos (directed complete partially ordered sets), and their relations with surjective dcpo maps (directed join preserving maps). The motivation of studying such infinitary ordered structures is their importance in domain theory, a theory on the borderline of mathematics and theoretical computer science.In this paper, introducing the notion of a pre-congruence on dcpos (directed complete partially ordered sets), we give a characterization of dcpo congruences. Also, it is proved that unlike natural dcpo congruences, the dcpo congruences are precisely kernels of surjective dcpo maps. Also, while it is known that the image of a dcpo map is not necessarily a subdcpo of its codomain, we find equivalent conditions on a dcpo map to satisfy this property. Moreover, we prove the Decomposition Theorem and its consequences for dcpo maps. 相似文献
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This study focuses on the early stages of the gelation of an aqueous type A (pig skin) gelatin solution. The thermo-reversible mono and triple helix formation was observed by rheology and proton NMR relaxation measurements. At high temperatures (T > 330 K), gelatin molecules form flexible random coils of small hydrodynamic radius, the elastic modulus of the solution is relatively low. On decreasing the temperature (330-320 K), mono helix formation begins, connected with an increase of the storage modulus and the hydrodynamic radius. The absence of a significant concentration dependence of this early variation of the modulus indicates the intramolecular nature of this structural change. The simultaneous decrease of the spin-spin relaxation times of the 1H signals of certain aminoacids confirms its effect on the molecular mobility. As this affects especially the signals of arginine and lysine, we conclude that these basic aminoacids play a significant role in forming the intramolecular interactions. The formation of a three-dimensional network occurs at a point at which the viscosity begins to increase rapidly near the gel point (T < 320 K). This process is clearly dominated by intermolecular interactions, as the slope as well as the starting point of the rapid increase significantly depends on the concentration. 相似文献