用手性高效液相色谱对β—羟基酯化合物进行对映体分离

本文利用手性ＯＤ柱在高效液相色谱上对光活性β－羟基酯类化合物进行了对映体分离，达到了较好的分离效果，并通过讨论手性ＯＤ涂附物的结构与该类化合物的作用方式，解释了不同的该类化合物的色谱分离效果。     

Development of an indirect competitive ELISA for the determination of papaverine

Papaverine (1-(3,4-dimethoxybenzyl)-6,7-dimethoxyisoquinoline, PAP) is a member of the benzylisoquinoline sub-group of the opium alkaloids. It has been widely used for treating diseases like pulmonary arterial embolism and renal or biliary colic. In this paper, a specific conjugate of mono-demethylated papaverine-O-carboxylmethyl ether (MDMPAP-O-CME) and bovine serum albumin (BSA) was synthesized and used as the complete antigen (PAP-BSA), with which we successfully obtained a high-titer anti-PAP polyclonal antibody (pAb) by immunization of rabbits. The anti-PAP pAb showed high affinity to papaverine with an affinity constant (K_aff) of 7.3 × 10⁷ L/mol. With this antibody, we established a sensitive immunochemical method for the determination of papaverine based on indirect competitive enzyme-linked immunosorbent assay (ELISA). The optimal concentrations of the coated antigen (PAP-OVA) and purified pAb used in the ELISA were 5 and 1.2 μg/mL, respectively. The cross reactivity of other benzylisoquinoline derived substances, including 1-(3,4-dihydroxybenzyl)-7-hydroxy-6-methoxy-isoquinoline (6-methoxy-papaveroline, MPAPO), morphine (MP) and codeine (CD) were all lower than 1%. The linear range of the calibration curve was 0.1-1000 ng/mL. Normal human serum samples were spiked with known amount of papaverine and measured by the ELISA. Recoveries were between 102% and 105%. Papaverine content in a commercial papaverine hydrochloride injection sample was also determined using the established ELISA. Compared with the results given by the control experiment of HPLC, the recoveries of ELISA to detect injection samples were 102-110%. The limits of detection for synthetic serum samples and injection samples of papaverine hydrochloride were 0.25 and 0.06 ng/mL, respectively.     

Study on the oligomerization of cyclopentadiene and dicyclopentadiene to tricyclopentadiene through diels-alder reaction

Summary The oligomerization of cyclopentadiene (CPD) and its dimer, dicyclopentadiene (DCPD), to tricyclopentadiene (TCPD) through Diels-Alder reaction at temperatures between 120 and 150°C was investigated. The results show that reaction temperature, pressure and solvent influence the product yield; at 150°C up to 50% yield of TCPD was obtained in the absence of solvents. The ratios of isomers A to B in the product can be adjusted by using different solvents. The kinetics indicate that the rate is more sensitive to the concentration of CPD than to that of DCPD.     

Mn(Ⅱ)/Mn(Ⅲ)电对在硫酸溶液中氧化还原行为

采用循环伏安法对Mn(Ⅱ)/Mn(Ⅲ)电对在硫酸溶液中铂电极上的氧化还原与Mn(Ⅱ)浓度、酸浓度、扫描速率、温度以及对流因素的函数关系进行了研究.结果发现,Mn(Ⅱ)在铂电极上Mn(Ⅱ)的氧化及Mn(Ⅲ)的还原均受扩散控制；升高温度和磁搅拌均能增加Mn(Ⅱ)氧化为Mn(Ⅲ)的速率；增加酸浓度和Mn(Ⅱ)浓度有利于增加Mn(Ⅲ)的稳定性,减少Mn(Ⅲ)的歧化和水解.     

多晶Hg0.09Cd0.91Te薄膜的光电化学行为

富镉Hg_(1-x)Cd_xTe是一种新型的光电转换材料,已用于固体结太阳能光伏电池:ITO/CdS/Hg_(1-)Cd_xTe/Au。我们曾对Hg_(1-x)Cd_xTe的电沉积机理作过研究。最近我们用电沉积制备的Hg_(0.09)Cd_(0.91)Te多晶薄膜做成了液体结太阳能光电化学电池并观察到明显光电响应。     

Experimental and theoretical study on the olefin metathesis of alkenyl Baylis-Hillman adducts using second-generation Grubbs catalyst

[reaction: see text] We have investigated the olefin metathesis from alkenyl Baylis-Hillman adducts using second-generation Grubbs catalyst. In the experiment, the ring-closing metathesis (RCM) product could not be found, while the cross-metathesis (CM) products were found. The computational studies provided consistent explanations for the experimental result. The most limiting factor for the RCM process using second-generation Grubbs catalyst is caused by the high strain and steric effect in the metallacyclobutane intermediates.     

Manipulating the surface properties of polyacrylamide with nitrogen plasma

Polyacrylamide (PAL) was physically adsorbed onto a hydroxylated silicon surface to form a uniform PAL film and the up-top PAL thin film was treated by nitrogen (N₂) plasma for surface modification. The atomic composition of the modified surface of the PAL film adsorbed on silicon substrate was analyzed with Fourier Transform Infrared Spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The surface energy of PAL film was calculated from the data of contact angle of three-probe liquid. The FTIR results show an increase of peak intensity at 1214 cm⁻¹ (NH₂ stretch vibration) after the nitrogen plasma treatment, which confirms that the nitrogen was grafted to the PAL surface in the process of N₂-plasma treatment. The XPS results show that the ratio of relative intensity of N1s to O1s increases with increasing the plasma treatment time, which further affirms the formation of the amine groups on the PAL surface after the nitrogen plasma treatment. The surface tension increases with increasing the plasma grafting time. However, the surface energy decreases rapidly at the early stage when stored in air and approaches to an equilibrium value. It suggests that some physically-adsorbed ions and alkyl radicals on PAL surface can rapidly lose their activities. The increase of the surface tension of the plasma treated PLA films is due to the amine groups covalently grafted to PAL surface.     

东太平洋深海锰结核的化学元素研究

用ICP-AES法测定了东太平洋深海锰结核样品中二十多种主，次及微量元素，结果表明，表面瘤状和表面光滑两种类型锰结核的化学元素含量有较明显的区别，如表面光滑的锰结核TiO2含较高，而表面瘤状的锰结核Mn/Fe之值较高等。     

Regioselective synthesis of polysubstituted benzenes from Baylis-Hillman adducts via [4+2] annulation protocol

A new and regioselective [4+2] benzannulation protocol toward polysubstituted benzenes was developed. A nitroalkane derivative, which was prepared from Baylis-Hillman adduct, served as the four-carbon unit and a Michael acceptor as a two-carbon unit. Vinyltriphenylphosphonium salt could also be used as a Michael acceptor efficiently.     

Mononuclear nonheme ferric-peroxo complex in aldehyde deformylation

A mononuclear nonheme ferric-peroxo complex bearing a macrocyclic tetradentate N4 ligand, [(TMC)Fe(III)-O2]+, was prepared and used in mechanistic studies of aldehyde deformylation; a catalytic aldehyde deformylation by a nonheme iron(II) complex, [Fe(II)(TMC)]2+, and molecular oxygen is reported as well.