共查询到20条相似文献,搜索用时 116 毫秒
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《液相色谱法及相关技术杂志》2012,35(11):2453-2460
Abstract Complete resolution of bufadienolides by traditional column chromatography, or by thin-layer chromatography (TLC), is quite difficult. Separation of various bufadienolide conjugates by high performance liquid chromatography (HPLC) has been described, but not for resolution of the corresponding genins. A preparative HPLC procedure has been developed for resolving the difficultly separable bufotalin (1) and cinobufotalin (2). A Lobar B column packed with LiChromprep Si-60 was used to separate these bufadienolides employing a recycling procedure. 相似文献
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《液相色谱法及相关技术杂志》2012,35(2):133-149
Abstract The correlation of the results of five thin-layer chromatographic techniques and their transfer to high performance liquid chromatography are reported. The data obtained indicate that high performance thin-layer chromatographic results are faithfully reproduced by high performance liquid chromatography. Although plates were not activated, and mixed solvents were used, no major differences were observed between thin layer and high performance liquid chromatography. 相似文献
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V. P. Shevchenko I. Yu. Nagayev N. F. Myasoyedov 《Journal of Radioanalytical and Nuclear Chemistry》1988,121(2):479-487
This is a comparative study of different chromatographic techniques [gas-liquid (GLC), thin-layer (TLC), liquid (LC), high-pressure liquid (HPLC) chromatography] as applied to the analysis and preparative purification of tritium-labelled eicosanoids with a molar radioactivity of 1.8–8.8 TBq/mmol obtained by selective hydrogenation and by chemical or enzymic methods. We demonstrate the possibility of analyzing reaction mixtures and isolating individual multiply labelled eicosanoids with a chemical and radiochemical purity of 95–98%. Special features of HPLC for high molar radioactivity eicosanoids are considered. 相似文献
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对从1979年至2005年期间植物皂甙的测定方法的进展作了评述。内容主要涉及薄层色谱法、气相色谱法、高效液相色谱法(HPLC)、HPLC与其他方法联用及毛细管电泳等,引用文献35篇。 相似文献
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M. Fenske 《Chromatographia》1997,44(1-2):50-54
Summary In order to collect urinary samples from unrestrained guinea pigs, animals were kept in their familiar home cages with wood
shavings for bedding. Cortisol was removed from shavings by a simple washing step, and an attempt was made to measure its
concentrations by high performance thin-layer chromatography (HPTLC), high performance liquid chromatography (HPLC), or thin
layer chromatography/radioimmunoassay (TLC-RIA). After intramuscular administration of 25 mg cortisol, cortisol excretion
increased from about 20–30 μg/day to 400–500 μg/day (HPTLC: 531 μg/day, HPLC: 493 μg/day; TLC-RIA: 394 μg/day). Similarly,
the treatment of the animals with 20 IU ACTH resulted in an augmented cortisol excretion, with mean values of 294 μg/day (HPTLC),
256 μg/day (HPLC) and 143 μg/day (TLC-RIA), respectively.
The present study shows, for the first time, that cortisol excretion in unrestrained laboratory animals can be determined.
Whilst the cortisol values measured by HPTLC and HPLC agree, the amounts measured by TLC-RIA were significantly lower. These
differences are probably due to the presence of substances in urine or shavings which interfere with the radioimmunological
determination. Hence, cortisol should be determined either by HPTLC or HPLC. Beside having a desirable specificity, these
methods are more suited than TLC/RIA for steroid analysis since they confer the possibility of measuring additional steroids
(e.g. precursors and/or metabolites of cortisol) in a single urine extract. This is especially the case for the HPTLC method
since substances can be transformed into fluorescent derivatives. 相似文献
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Methods for the separation of peptides on a new type of thin-layer chromatography (TLC) sheet and blotting onto polyvinylidene difluoride (PVDF) membranes with subsequent gas phase sequencing are described. For validation, the A and B chain of insulin were chromatographed on Empore TLC sheets and either extracted or blotted onto PVDF membranes. The advantages and disadvantages of thin-layer chromatography on Empore sheets versus high performance liquid chromatography (HPLC) are discussed, along with the possibility of combining the two methods. In addition, TLC was combined with electrophoresis (fingerprinting) for the separation of complex peptide mixtures. Blotting from TLC sheets onto PVDF membranes was performed in two ways: contact diffusion and electrophoretic transfer. In our experiments electroblotting was more effective. Amino acid sequence determination of the B chain of insulin was possible both after extraction from the TLC sheet and after blotting onto PVDF membranes. In the former case, liquid phase sequencing and, in the latter case, gas phase sequencing was performed. The possibility to blot from TLC sheets onto membranes, e.g. PVDF, may prove useful in many fields, for example in biochemistry, and in molecular and cell biology. 相似文献
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The chromatographic properties of 51 common pesticides have been measured using seven different chromatographic systems involving gas-liquid chromatography (GLC), high-performance liquid chromatography (HPLC) with diode-array spectrophotometric detection and thin-layer chromatography (TLC) with different spray reagents. Correlation coefficients were calculated for combinations of all systems. The best combination of the chromatographic systems examined for the identification of an unknown compound is GLC on OV-17, HPLC on ODS-Hypersil with acetonitrile-water as eluent and TLC using an isooctane-ethyl acetate solvent system. 相似文献
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A method for the determination of theophylline (TH), without derivatization, in serum by isotope dilution mass spectrometry
using labelled [1, 3-15N2-2-13C]theophylline (LTH) as internal standard is described. After deproteinization, the analyte is directly injected into a high
performance liquid chromatography – mass spectrometer operating with atmospheric-pressure chemical-ionization (APCI HPLC/MS).
The concentrations of TH in sera measured by APCI HPLC/MS are compared with results from gas chromatography – isotope dilution
mass spectrometry (GC-ID/MS), high performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA).
The accuracy, precision and recovery of the APCI HPLC/MS and GC-ID/MS methods are discussed. The coefficient of variation
(CV) determined from duplicate samples was less than 2%. The detection limit was 10 ng/ml at a signal-to-noise ratio of 3:1.
Received: 17 January 1996/Revised: 26 March 1996/Accepted: 5 April 1996 相似文献
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JPC – Journal of Planar Chromatography – Modern TLC - Simple, accurate, precise, sensitive, and validated high-performance liquid chromatography (HPLC) and thin-layer chromatography... 相似文献
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Identification problems often encountered in high performance liquid chromatography (HPLC)/Fourier transform infrared spectrometry (FTIR) can be circumvented through the use of a thin-layer chromatographic (TLC) plate as deposition and infra-red sampling medium. The combination of complementary separation modes is shown to demonstrate increased resolution of the components of complex mixtures. In this particular work, the effluent from a reversed-phase microcolumn is continuously deposited on a TLC plate with alumina stationary phase. The solute remains on the plate as a continuous record of the HPLC separation, which is then analyzed by diffuse reflectance FTIR. When the HPLC separation is inadequate for full separation of the components, the immobilized HPLC chromatogram serves as a starting point for subsequent TLC separation. A number of FTIR reconstructed chromatograms and spectra which are derived from the TLC plate aid in the interpretation. 相似文献
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《Analytical letters》2012,45(7):1439-1449
Abstract In this work, the (R)‐N,N,N‐trimethyl‐2‐aminobutanol‐bis(trifluoromethanesulfon)imidate chiral ionic liquid was first used in chromatography and exhibited a excellent chiral recognition ability in high performance capillary electrophoresis (HPCE), high performance liquid chromatography (HPLC), and capillary gas chromatography (CGC), which also showed for the first time that chrial ionic liquid was an effective chiral selector in HPCE and HPLC. The compounds that have been separated using this chiral ionic liquid at least included alcohol, amine, acid, and amino acid, et al. enantiomers. As the chiral ionic liquid can be easily synthesized from relatively inexpensive materials, it should have a great potentiality for chiral separation in chromatographic science. 相似文献
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H Oka Y Ikai N Kawamura K Uno M Yamada K Harada M Uchiyama H Asukabe M Suzuki 《Journal of chromatography. A》1987,393(2):285-296
Analytical methods for eight tetracyclines (TCs) were established using silica gel high-performance thin-layer chromatography (HPTLC), reversed-phase thin-layer chromatography (RP-TLC) and high-performance liquid chromatography (HPLC). Good separations of eight TCs were obtained using chloroform-methanol-5% disodium ethylenediaminetetraacetate solution (65:20:5) (lower layer) and methanol acetonitrile 0.5 M oxalic acid solution (1:1:4) (pH 3.0) on silica gel HPTLC and C8 TLC plates, respectively. A combination of HPTLC and RP-TLC made possible the identification of the eight TCs. Each calibration graph was linear between 0.1 and 1.0 microgram using UV densitometry except for rolitetracycline. For detection reagents, the diazonium salts including Fast Violet B gave variously coloured spots with the eight TCs and good sensitivities were obtained except with minocycline. In HPLC, the simultaneous analysis of the eight TCs on a C8 column was possible using methanol-acetonitrile-0.01 M oxalic acid solution (1:1.5:7) adjusted to pH 3.0 as the mobile phase. A linear relationship was obtained between 1.0 and 10 ng using the usual sample preparation except for rolitetracycline. The direct determination of rolitetracycline was possible using tetrahydrofuran, dimethyl sulphoxide and the mobile phase as solvents for preparation of the sample. For the determination of residual rolitetracycline, it was effective to measure the amount of rolitetracycline as tetracycline by HPLC, HPTLC and RP-TLC after conversion of rolitetracycline to tetracycline by incubating for 5 min in methanol at 50 degrees C. 相似文献
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Chromatographic methods as tools in the field of mycotoxins 总被引:1,自引:0,他引:1
V Betina 《Journal of chromatography. A》1989,477(2):187-233
Achievements in the applications of chromatographic techniques in mycotoxicology are reviewed. Historically, column chromatography (CC) and paper chromatography (PC) were applied first, followed by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatography (GC). Although PC techniques are no longer used in the analysis of mycotoxins, selected applications of PC are included to underline historical continuity. The most important achievements published from 1980 onwards are described. They include clean-up methods, TLC, CC, HPLC and GC of mycotoxins in environmental samples, foods, feeds, body fluids and in studies on biosynthesis and biotransformations of mycotoxins. Advantages and disadvantages of chromatographic techniques used in mycotoxicology are also evaluated. 相似文献
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《液相色谱法及相关技术杂志》2012,35(7):1207-1218
Abstract Fatty alcohols were obtained from the saponified chloro-form-methanol extract of Mycobacterium tuberculosis H37Ra by extraction with diethyl ether and partially purified by silicic acid column chromatography. The long-chain fatty alcohols (C49-C58) were separated from the shorter-chain alcohols by Sephadex LH-20 column chromatography and further fractionated into saturated and unsaturated alcohols by argentation thin-layer chromatography. These two fractions were analyzed by proton nuclear magnetic resonance spectro-scopy, derivatized to the 3,5-dinitrobenzoyl esters, and fractionated on a C18-bonded silica column by high performance liquid chromatography (HPLC). Complete separation of esters differing by two carbon units and partial separation 相似文献