儿童脑病高压氧治疗的护理

对30例进行高压氧治疗的脑病患儿给予进舱治疗前、治疗时及治疗后护理,使患儿能安全、顺利完成多个疗程的高压氧治疗,从而得到康复,取得满意的临床效果。     

急性脑梗死介入治疗术后护理

总结了15例急性脑梗死患者动脉介入治疗术后的护理体会,阐述了术后在心理护理、呼吸道护理、持续抗凝治疗、防止脑出血以及出院指导、康复锻炼等方面所采取的措施,这些是预防重要并发症发生,使患者顺利康复的重要因素。     

肿瘤患者化疗后骨髓抑制的护理探讨

通过对113例化疗后骨髓抑制患者采用保护性隔离，皮肤、口腔、上呼吸道、泌尿道护理及饮食、心理等综合性护理措施的观察，发现113例化疗后骨髓抑制患者，经过综合护理措施仅13例出现感染、发热，2例经抗生素治疗无效，死于感染性休克，其余患者的血象均恢复正常，康复出院。所以本护理措施，对促进化疗后骨髓抑制患者的康复有一定的应用价值。     

综合治疗脑梗塞68例观察

脑梗塞是常见病，且大部分可引起偏瘫，失语，智能障碍等后遗症，严重影响病人的生存质量，广州市第六人民医院于１９９５年１月～１９９７年６月采用中西药物，传统治疗，功能障碍治疗，心理治疗，氦氖激光血管内照射，血液光量子疗法，高压氧等综合治疗脑梗塞６８例，总疗程３个月，结果，总有效率９４．１％，显效率６４．１％，明显高于单用药物治疗的显效率，说明综合治疗可提高疗效，减少后遗症及致残率，且病程越短，疗效越好     

整体护理在缺铁性贫血护理中的应用体会

目的探究为缺铁性贫血患者行整体护理干预对其治疗依从性和护理满意度的影响。方法选取江西省宜春市人民医院于2015年11月至2016年11月期间收治的76例缺铁性贫血患者参与研究,将其随机分为对照组(n=38)和研究组(n=38),患者入院即刻开始实施护理,分别实施常规护理及整体护理。结果护理后3周研究组患者的治疗依从性与护理满意度均明显高于对照组,组间数据差异存在统计学意义(P0.05)。结论整体护理应用于缺铁性贫血患者中对其病情控制具有正面影响,还可提升患者的治疗依从性及护理满意度,可扩大应用范围。     

酒精中毒的联合救治与护理

对18例单纯酒精中毒者进行了临床治疗并对其治疗方法，护理方法及心理护理等进行了总结与讨论。     

延续性护理对缺血性脑卒中患者复发及治疗依从性的影响

目的探讨分析延续性护理干预对缺血性脑卒中患者复发及治疗依从性的影响。方法随机选取辽宁省抚顺市东洲区龙凤街道社区卫生服务中心62例缺血性脑卒中患者,将其分为常规组与实验组各31例,常规组实施常规护理,实验组实施延续性护理干预,比较两组患者治疗依从性及疾病复发情况。结果随访1年,实验组患者科学运动、合理饮食、规范用药等治疗依从性明显高于常规组(P0.05);实验组患者生活质量评分为(8.84±0.62)分,明显优于常规组(6.37±0.40)分(P0.05);实验组复发率为6.45%,显著低于常规组19.35%(P0.05)。结论对缺血性脑卒中患者实施延续性护理干预,能够有效提高患者治疗依从性及生存质量,降低疾病复发率,值得推广普及。     

急性心肌梗死患者焦虑症用药及心理护理的临床价值

目的对急性心肌梗死患者焦虑症调查,探讨用药及心理护理的临床价值。方法研究对象选取孝感市中心医院2013年11月—2014年11月收治的84例急性心肌梗死患者,随机分为对照组与观察组(各42例)。对照组患者接受常规护理;观察组同时给予用药护理和心理护理。对全体患者用药相关焦虑情况进行调查,统计两组患者护理后用改良版药依从性行为量表(Morisky)评分和焦虑自评量表(SAS)评分。结果用药依从性高组患者SAS评分(53.74±6.15)分显著低于用药依从性低组(P0.05);护理后,较对照组,观察组患者用Morisky评分(13.52±2.23)分高,SAS评分(42.37±5.24)分低,两组比较差异有统计学意义(P0.05)。结论急性心肌梗死患者用药依从性差与焦虑发生有显著相关,给予用药及心理护理能有效提高患者用药依从性,改善焦虑心理。     

心理护理干预在CT增强扫描质量控制中的影响

目的探讨心理护理干预在CT增强扫描质量控制中的应用价值。方法共461例患者列入本研究,研究组231例,对照组230例。研究组在对照组基础上进行心理护理干预。对两组患者行检查前血压、心率、心理状态变化情况;检查后所获得的CT图像质量和增强效果进行对比。结果研究组检查前收缩压、心率波动、干预后HAMA评分、CT图像运动性伪影的出现次数均低于对照组,而增强效果优于对照组;两组比较有显著性差异(P0.05)。结论心理护理干预有助于减轻CT增强扫描患者的焦虑心理,使患者各项指标趋于正常,确保检查顺利进行及CT图像质量和增强效果。     

高压氧治疗糖尿病足的疗效分析

为观察高压氧(HBO)治疗糖尿病并发糖尿病足的临床疗效,HBO组36例患者在使用抗生素及局部换药等治疗的同时,采用HBO治疗;对照组34例患者仅使用抗生素及局部换药等治疗。结果表明,HBO组患者总的有效率为91.7%,而对照组总有效率为67.5%,两组的疗效有显著性差异(P<0.05)。HBO对糖尿病足有显著的疗效,值得临床进一步研究和应用。     

Revealing the ionization ability of binding site I of human serum albumin using 2-(2'-hydroxyphenyl)benzoxazole as a pH sensitive probe

The ability of site I of human serum albumin (HSA) to bind medium sized molecules is important for the distribution, metabolism, and efficacy of many drugs. Herein, we show that this binding site has the ionization ability that may alter the drug structure during the process of its delivery. We reveal this ability by employing 2-(2'-hydroxyphenyl)benzoxazole (HBO) as a pH sensitive probe. Binding of HBO in site I is studied here at physiological pH 7.2 using steady-state and lifetime spectroscopic measurements, molecular docking and molecular dynamics (MD) simulation methods. The complex photophysics of HBO and the unique fluorescence signature of its anionic form indicate that, upon binding with HSA, the molecule exists in equilibrium between the anionic and the syn-keto forms. The position of HBO inside the binding site was determined experimentally by measuring the fluorescence quenching of W214, the sole tryptophan residue in HSA. The ionization degree of HBO inside the binding site was estimated to be close to the ionization degree of HBO in an aqueous solution of pH 10. This was concluded by comparing the fluorescence behavior of bound HBO to that of HBO in different solvents and in aqueous solutions of different pH values. Molecular docking and MD simulations show that HBO binds in site I close to W214, confirming the experimental results, and pinpoint the dominant role of hydrophobic interactions in the binding site. The formation of the anionic form is proposed to be due to through-space interaction between the OH group of HBO and both R222 and I290 with a binding mode similar to that of warfarin in site I. Comparison of the results with those of HBO mixed with key amino acids in solution indicates the importance of through-space interaction in the formation of the anion, similar to enzymatic reactions.     

A theoretical prediction about harnessing ESPT process for HBO derivatives

The different excited-state behaviors involved in excited-state proton transfer (ESPT) process of a series of 2-(2-hydroxyphenyl)benzoxazole (HBO) derivatives have been theoretically investigated. The primary bond lengths and bond angles were analyzed. Coupling with the infrared (IR) vibrational spectra, we confirmed that the intramolecular hydrogen bond O–H···N should be strengthened in the S₁ state, which might provide the possibility for ESPT reaction, whereas introducing the fused rings may weaken the hydrogen bond in excited state. By investigating the vertical excitation process, the charge redistribution was explored. It is found that the electron-accepting –NO₂ and –COOH would facilitate the ESPT reaction. With adding fused rings to HBO, less charge transfer exists in the transition process, which can reasonably explain the weakening hydrogen bond phenomenon in excited states. Via constructing the potential energy curves of both S₀ and S₁ states, we further confirm that electron-accepting substitutions could promote the ESPT process for HBO systems. And fused rings do inhibit ESPT reaction to a great extent. We believe this work not only elaborates the different excited-state proton transfer behaviors for a series of HBO derivatives but also presents a new harnessing ESPT process through substitutional effects.     

Differential solvation and tautomer stability of a model base pair within the minor and major grooves of DNA

2-(2'-Hydroxyphenyl)benzoxazole (HBO) may be used as a model base pair to study solvation, duplex environment, and tautomerization within the major and minor groves of DNA duplexes. In its ground state, HBO possesses an enol moiety which may be oriented syn or anti relative to the imino nitrogen of the benzoxazole ring. In the absence of external hydrogen-bond donors and acceptors HBO exists as the internally hydrogen-bonded syn-enol, a mimic of the rare base pair tautomer found in DNA, which may be photoinduced to tautomerize and form the keto tautomer, a mimic of the dominant base pair tautomer. Previously, we demonstrated that when incorporated into DNA such that the enol moiety is positioned in the major groove, HBO is not solvated, exists exclusively as the internally hydrogen-bonded syn-enol which is efficiently photoinduced to tautomerize, and the corresponding keto tautomer is preferentially stabilized. In stark contrast, we now show that when HBO is incorporated in DNA such that the enol moiety is positioned in the minor groove, the enol tautomer is preferentially stabilized. Molecular dynamics simulations suggest that this results from the formation of a stable hydrogen-bond between the HBO enol and the O4' atom of an adjacent nucleotide, an H-bond acceptor that is only available in the minor groove. The differential stabilization of the enol and keto tautomers in the major and minor grooves may reflect the functions for which these environments evolved, including duplex replication, stability, and recognition.     

Rotational energy barrier of 2-(2',6'-dihydroxyphenyl)benzoxazole: a case study by NMR

2-(2'-Hydroxyphenyl)benzoxazole (HBO) derivatives represent an important class of luminescent materials, as they can undergo excited state intramolecular proton transfer (ESIPT). The material's ESIPT properties are dependent on the ratio of two different rotamers, whose interconversion is poorly understood. By using HBO derivative 4, the rotational energy barrier of 2- (2',6'-hydroxyphenyl)benzoxazole is determined to be 10.5 kcal/mol by variable-temperature NMR. Although a HBO derivative typically exhibits two rotamers with O···H-O (e.g., 1a) and N···H-O bonding (e.g., 1b), correlation of NMR with fluorescence data reveals that the rotamer with N···H-O bonding is predominant in the solution.     

Cooperative hydrogen bonding in trimers involving HCN and HBO

A computational study of the cooperative effect of hydrogen bonding in linear trimers comprised of HCN and HBO molecules was undertaken at the MP2/6-311++G(2d,2p) level of theory. It was found that the third molecule leads to enhancement of the binding relative to that of the dimer species comprising these monomers. HBO is a better proton acceptor and a weaker proton donor than HCN.     

On the interconversion pathway of HBO<-->BOH

The potential energy surfaces have been constructed for the 1A', 3A', and 3A" states of HBO by using the multireference perturbation theory with the basis set cc-pVTZ (6d,10f). Two stationary points and a transition state have been characterized on all the three surfaces, which are in good agreement with available experiments and previous calculations. The interconversion pathways from metastable boron hydroxide BOH to the considerably more stable HBO are expounded based on the nature of the surfaces.     

The role of tautomeric and rotameric species in the photophysics of 2-(2′-hydroxyphenyl)benzoxazole

The complex photophysical behaviour of 2-(2′-hydroxyphenyl)benzoxazole (HBO) has been investigated using both steady-state absorption and fluorescence and time-resolved (picosecond) emission spectroscopy, and found to be consistent with the existence of various tautomeric and rotameric species in equilibrium in the ground state. The natures of these species are discussed. Molecular-orbital calculations (PPP), performed for all species, gave results in good agreement with experiment. Excited-state intramolecular proton transfer was found to occur with a rate constant exceeding 1 × 10¹¹ s^?1.     

Application of equation-of-motion coupled-cluster methods to low-lying singlet and triplet electronic states of HBO and BOH

The equilibrium structures and physical properties of the X (1)sigma(+) linear electronic states, linear excited singlet and triplet electronic states of hydroboron monoxide (HBO) (A (1)sigma(-), B (1)delta, a (3)sigma(+), and b (3)delta) and boron hydroxide (BOH) (A (1)sigma(+), B (1)Pi, and b (3)Pi), and their bent counterparts (HBO a (3)A('), b (3)A("), A (1)A("), B (1)A(') and BOH X (1)A('), b (3)A('), c (3)A("), A (1)A('), B (1)A('), C (1)A(")) are investigated using excited electronic state ab initio equation-of-motion coupled-cluster (EOM-CC) methods. A new implementation of open-shell EOM-CC including iterative partial triple excitations (EOM-CC3) was tested. Coupled-cluster wave functions with single and double excitations (CCSD), single, double, and iterative partial triple excitations (CC3), and single, double, and full triple excitations (CCSDT) are employed with the correlation-consistent quadruple and quintuple zeta basis sets. The linear HBO X (1)sigma(+) state is predicted to lie 48.3 kcal mol(-1) (2.09 eV) lower in energy than the BOH X (1)sigma(+) linear stationary point at the CCSDT level of theory. The CCSDT BOH barrier to linearity is predicted to lie 3.7 kcal mol(-1) (0.16 eV). With a harmonic zero-point vibrational energy correction, the HBO X (1)sigma(+)-BOH X (1)A(') energy difference is 45.2 kcal mol(-1) (1.96 eV). The lowest triplet excited electronic state of HBO, a (3)A('), has a predicted excitation energy (T(e)) of 115 kcal mol(-1) (4.97 eV) from the HBO ground state minimum, while the lowest-bound BOH excited electronic state, b (3)A('), has a T(e) of 70.2 kcal mol(-1) (3.04 eV) with respect to BOH X (1)A('). The T(e) values predicted for the lowest singlet excited states are A (1)A(")<--X (1)sigma(+)=139 kcal mol(-1) (6.01 eV) for HBO and A (1)A(')<--X (1)A(')=102 kcal mol(-1) (4.42 eV) for BOH. Also for BOH, the triplet vertical transition energies are b (3)A(')<--X (1)A(')=71.4 kcal mol(-1) (3.10 eV) and c (3)A(")<--X (1)A(')=87.2 kcal mol(-1) (3.78 eV).