滇产玉米须化学成分研究

为研究滇产玉米须中的化学成分,采用硅胶柱层析和Sephadex LH-20柱层析进行化合物分离和纯化,采用EI-MS、HR-ESI-MS、1D-和2D-NMR以及化学方法对化合物结构进行鉴定。从滇产玉米须中分离得到了4个化合物,分别鉴定为:(2S,3S,4R)-2-[(2’R)-2’-hydroxydocosanoylamino]-1,3,4-octadecanetriol(1),ent-rosa-ne-5β,15,16-triol(2),麦角甾-7,22-二烯-3β,5α,6β-三醇(3),豆甾-5-烯-3β,7α-二醇(4)。化合物1和2为首次从玉米须中分离得到,并采用化学方法对化合物1进行结构确证,采用2D-NMR对这化合物2和3的碳氢数据进行准确归属。     

崖县风车子茎化学成分研究

研究崖县风车子茎的化学成分。用硅胶柱层析法、制备薄层色谱法、重结晶法和高效液相色谱法进行分离,并根据理化性质和光谱数据对分离化合物进行结构鉴定。分离并鉴定了10个化合物,分别为:3,3',4'-tri-O-methylellagic acid(1),3,3'-di-O-methylellagic acid(2),hederagenin(3),白桦脂酸(4),6R,9R-blumenol B(5),6S,9R-blumenol B(6),2α,3β,23-trihydroxyolean-12-en-28-oic(7),4-hydroxycinnamic acid(8),咖啡酸(9),evofolin B(10)。化合物5、6、9和10为首次从该属植物中分离得到,所有化合物均为首次从该植物中分离得到。     

海南暗罗根中一个新的克罗烷型二萜

采用硅胶柱层析,制备薄层层析等色谱方法,从海南暗罗根中分离得到8个二萜类化合物,分别鉴定为polyalimide A(1),kolavenic acid(2),2-oxo-kolavenic acid(3),2-oxo-14,15-bisnor-3,11E-kolavadien-13-one(4),3,4-dihydroxyclerodan-13E-en-15-oic acid(5),longimide B(6),16-oxocleroda-3,13E-dien-15-oic acid(7),solidagonal acidmethyl ester(8).其中化合物1为新化合物,化合物5首次从番荔枝科分离得到,所有化合物首次从该植物中分离得到.化合物2和3对人肿瘤细胞SPCA-1,SGC-7901和K-562显示微弱的细胞毒性.     

云南萝芙木花新吲哚生物碱

从云南萝芙木(Rauvolfia yunnanensis Tsiang)花乙酸乙酯提取物中分离得到9个化合物(其中4个为吲哚类生物碱), 即(18Z)-11-methoxypicrinine(1), caberoline(2), 11-methoxystrictamine(3)和strictamine(4), 以及russulamide(5), ursolic acid(6), urs-12(13)-en-3α-yl acetate(7), β-amyryl nonanoate(8)和β-sitosterol(9); 采用UV, IR, MS和1D, 2D NMR方法对化合物的结构进行了鉴定, 其中化合物1为新吲哚生物碱, 化合物5, 7和8系首次从该植物中分离得到, 但未得到利血平和育亨宾.     

天葵子的化学成分研究

植物中很少存在含氰基官能团的化合物。近期文献报道从常用抗癌中药天葵子中分离得到部分此类化合物，而且天葵子富含具有氰基官能团的化合物。为了丰富此类化合物的种类和数量，同时深入对中国药典收载品种的进一步研究，本文对天葵子的化学成分进行了系统分离。从中药天葵子中分离得到两个新化合物：一个为新的生物碱类化合物，命名为天葵碱A (1)，另一个为新的酯类化合物，3-(4'-羟基苯基)- 2-丙烯酸-(4'-羰基苯基)酯(2)。同时分离到了四个含氰基官能团的化合物：(Z)-6α-(β-D-glucosyloxy)-4α, 5α-dihydroxy-2- cyclohexene- Δ¹, α-acetonitrile (3), (Z)-6α-(β-D-glucosyloxy)- 4α-hydroxy-2-cyclohexene-Δ¹, α- acetonitrile (4), lithospermoside (5), ehretioside B (6)和11个已知化合物。新化合物1和2的结构主要通过一维和二维核磁共振技术确证。含氰基官能团的化合物3和4首次从毛茛科植物中分离得到。迄今为止，包括文献报道的部分化合物，从天葵子植物中共分离鉴定了6个比较少见的含氰基官能团的化合物。     

内生真菌叶下珠生拟茎点霉的次级代谢产物研究（英文）

从广藿香内生真菌叶下珠生拟茎点霉(PhomopsisphyllanthicolaA658)中分离纯化得到6个聚酮类化合物,分别鉴定为dothioreloneO(1),2-[3,5-二羟基-2-(7-羟基辛酰基)苯基]乙酸(2),dothioreloneM(3),dothioreloneK(4),cytosporoneB(5),和cytosporoneC(6).其中化合物1为新化合物,化合物2为新天然产物,新化合物的结构进一步通过HR-ESIMS,1DNMR和2DNMR等光谱技术确定.此外,对分离所得的化合物进行了体外细胞毒测试,结果表明化合物3～5显示出不同程度的细胞毒活性.     

地不容生物碱成分研究

为了研究地不容的生物碱成分,采用硅胶柱层析和Sephadex LH-20柱层析进行化合物的分离纯化,采用HRESIMS,NMR以及比旋光度测定等方法对化合物结构进行鉴定。从地不容中分离鉴定了7个化合物,包括3个吗啡型生物碱:青藤碱(1),sinomenine N-oxide(2)和青风藤碱(3);4个原小檗碱型生物碱:四氢巴马汀(4),紫堇单酚碱(5),千金藤定碱(6)和异紫堇单酚碱(7)。其中化合物2和7为首次从地不容中分离得到,化合物3对乙酰胆碱酯酶具有明显的抑制作用(IC50=3.03μM)。     

岩生石仙桃化学成分研究

采用柱色谱方法首次对岩生石仙桃80%乙醇提取物的乙酸乙酯部位进行分离纯化,利用波谱技术对分离得到的化合物进行结构鉴定。从岩生石仙桃乙酸乙酯部位中分离得到11个化合物,分别鉴定为棕榈酸甲酯(1),油酸甲酯(2),正癸烷(3),1-十二醇(4),β-谷甾酮(5),香草醛(6),4-羟-3,5-二甲氧基苯甲醛(7),3,5-二甲氧基-4-羟基-苯丙酮(8),对羟基苯丙酸甲酯(9),2,5-二甲氧基苯醌(10),ephemeranthoquinone(11)。化合物(1~11)均首次从该植物中分离得到,其中,化合物1~4和9,10首次从石仙桃属植物中分离得到。     

团状雪灵芝的化学成分研究

对石竹科蚤缀属植物团状雪灵芝全草的化学成分进行分离鉴定。采用色谱法分离纯化出5个化合物,运用波谱方法将它们的结构分别鉴定为二十八烷醇(1)、羊齿烯醇(2)、羊齿烯酮(3)、麦角甾醇-5,8-过氧化物(4)和小麦黄素(5)。这些化合物均为首次从该植物中分离得到,其中化合物1为首次从石竹科植物中分离得到,化合物2为首次从该属植物中分离得到。     

人面果树皮中口 山 酮 化合物及其DPPH自由基的清除活性

从人面果乙酸乙酯提取物中分离出5个新的口 山 酮 化合物garcinenone A (1), B (3), C (4), D (7) and E (8)和7个已知化合物,其结构经过波谱技术,特别是2D-NMR技术来鉴定。Jacareubin (2), 1,4,6-trihydroxy-5-methoxy-7-(3-methyl-2-buteny1)xanthone (6), subeliptenone B (11) 和symphoxanthone (12)为首次从该植物中分离出来。在DPPH自由基的清除活性实验中,所有的化合物都显示抗氧化活性,其IC50的值在 6.0-23.2 mM。结果表明人面果是潜在的有前景的天然抗氧化剂。     

A new diphenyl ether from Phoma sp. strain, SHZK-2

A new diphenyl ether methyl 2-(2-formyl-3-hydroxy-5-methylphenoxy)-5-hydroxy-3-methoxybenzoate (3), together with four known compounds, asterric acid (1), methyl asterrate (2), 9(Z),12(Z)-nonadecadienoic acid (4) and orsellinic acid (5), were isolated from the Phoma sp. strain SHZK-2, which was isolated from a polluted environment in southern China. The structures of these compounds were determined by spectroscopic methods. Cytotoxicities of compounds against HEPG2 cell and Raji cell lines were preliminarily evaluated by the MTT method.     

New benzoyl glucosides and cytotoxic pterosin sesquiterpenes from Pteris ensiformis Burm

Three new compounds: 2R,3R-pterosin L 3-O-beta-D-glucopyranoside (1), beta-D-xylopyranosyl(1-->2)-7-O-benzoyl-beta-D-glucopyranoside (2) and 4-O-benzoyl-beta-D-xylo-pyranosyl(1-->2)-7-O-benzoyl-beta-D-glucopyranoside (3), together with nine known compounds, were isolated from the ethyl acetate extract of Pteris ensiformis. 5-[2-Hydroxyethylidene]-2(5H)-furanone (4), which had been synthesized, was isolated from natural sources for the first time. The structures of all isolated compounds were determined on the basis of mass and spectroscopic evidence. Compound 1 and pterosin B (5) show cytotoxicity against HL 60 cells (human leukemia) with the IC(50) values of 3.7 and 8.7 microg/mL, respectively.     

Naucline, a new indole alkaloid from the bark of Nauclea officinalis

A new indole alkaloid, naucline (1) together with four known alkaloids, angustine (2), angustidine (3), nauclefine (4) and naucletine (5), were isolated from the bark of Nauclea officinalis. The structures of all isolated compounds were elucidated with various spectroscopic methods such as 1D- and 2D- NMR, IR, UV and LCMS-IT-TOF. In addition to that of alkaloid 1, the complete 13C-NMR data of naucletine (5) were also reported. Naucline (1) showed a moderate vasorelaxant activity (90% relaxation at 1 × 10(-5) M) whereas, angustine (2), nauclefine (4), and naucletine (5) showed potent vasorelaxant activity (more than 90% relaxation at 1 × 10(-5) M) on an isolated rat aorta.     

Aromatic compounds from the liverwort Conocephalum japonicum

Two undescribed dimeric ArC2 derivatives, cis- and trans-1,2-bis(3,4-dimethoxyphenyl)cyclobutane (1 and 2), one new monoterpenes esters, 2alpha,5beta-dihydroxybornane-2-cis-cinnamate (3), along with eight known compounds, 2alpha,5beta-dihydroxybornane-2-trans-cinnamate (4), perrottetin E (5), isoriccardin C (6), marchantin A (7), marchantin E (8), marchantin C (9), and isomarchantin C (10) were isolated from the liverwort Conocephalum japonicum. All the structures were established by extensive spectroscopic analysis. The isolated compounds 3-10 were evaluated for their cytotoxicity against the human KB cell line with IC50 values ranging from 16.5 to 50.2 microM.     

川明参茎叶中的化学成分

川明参 ( Chuanminshen violaceum Sheh et Shan)属于伞形科川明参属 ,在形态上与明党参相近 ,是我国特有的单种属植物 ,主要分布在四川 ,多为栽培 [1] .其根作为滋补药材 ,具有润肺化痰、和胃生津和解毒等功效 .饶高雄 [2 ] 及周燕等 [3] 曾对不同产地的川明参根部化学成分进行了研究 ,分离鉴定出的主要成分为芦丁和多种香豆素 .为了进一步探索川明参的活性成分以及对川明参资源的综合开发利用 ,我们对一般弃置不用的川明参茎叶的化学成分进行了研究 ,从其水煮提取物中分离得到 8个化合物 ,通过波谱分析鉴定了其中 7个化合物 ,它们是 4-…     

New sterols and triterpenoids from four edible mushrooms

Four edible mushrooms, Panellus serotinus, Lepista nuda, Tricholoma matsutake and Naematoloma sublateritium, have been investigated chemically. Two new sterols, 5alpha,9alpha-epidioxy-(22E)-ergosta-7,22-diene-3beta,6alpha-diol (1) and 5alpha,9alpha-epidioxy-(22E)-ergosta-7,22-diene-3beta,6beta-diol (2), have been isolated from Panellus serotinus. Compound 2 was also isolated from Lepista nuda. A new sterol, 3beta,5alpha,9alpha,14beta-tetrahydroxy-(22E)-ergosta-7,22-dien-6-one (3), and compound 2 have been isolated from Tricholoma matsutake. Three new triterpenoids, sublateriols A-C (4-6), have been isolated from Naematoloma sublateritium. The structures of the new compounds were elucidated on the basis of their spectral data.     

New abietane-type diterpenes from the heartwood of Picea morrisonicola

New abietane-type diterpenes, 15-acetoxy-7-oxodehydroabietic acid (1), picealactones A (2), B (3), and C (4), together with the known 7-oxodehydroabietic acid (5) were isolated and identified from the heartwood of Picea morrisonicola. The structures of 1-4 were determined on the basis of spectral data explanation. Compounds 2-4 possessed a rare 5-dehydro-18, 6-olide functionality. Compounds 1 and 2 were first isolated from natural source.     

Phenolic compounds and antioxidant activities of Liriope muscari

Five phenolic compounds, namely N-trans-coumaroyltyramine (1), N-trans-feruloyltyramine (2), N-trans-feruloyloctopamine (3), 5,7-dihydroxy-8-methoxyflavone (4) and (3S)3,5,4'-trihydroxy-7-methoxy-6-methylhomoisoflavanone (5), were isolated from the fibrous roots of Liriope muscari (Liliaceae). Compounds 2-5 were isolated for the first time from the Liriope genus. Their in vitro antioxidant activities were assessed by the DPPH and ABTS scavenging methods with microplate assays. The structure-activity relationships of compounds 1-3 are discussed.     

New lignan glycosides from Chinese medicinal plant,Sinopodophillum emodi

Two new podophillotoxin glucosides, L-picropodophillotpxin 7'-O-(beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside) (2) and L-picropodophillotpxin 7'-O-beta-D-glucopyranoside (3), were isolated from Chinese medicinal plant, Sinopodophillum emodi, together with 4 known compounds, podophillotoxin (1), podorhizol 4'-O-beta-D-glucopyranoside (4), deoxypodophillotoxin (5), and dehydropodophillotoxin (6). The structures of 2 and 3 were finally determined by the extensive decouping and nuclear Overhauser effect (NOE) experiments in NMR spectra and circular dichroism (CD) spectra. Compound 2 is the second example of podophillotoxin diglucoside, and both the first one and 2 were isolated from S. emodi. X-ray crystal structure analysis of 1, 5, and 6 was carried out. Compounds 1 and 5 showed the different conformations from those reported.     

Chemical constituents of malagasy liverworts, part V: prenyl bibenzyls and clerodane diterpenoids with nitric oxide inhibitory activity from Radula appressa and Thysananthus spathulistipus

3Beta,4beta:15,16-diepoxy-13(16),14-clerodadiene (1) and a new clerodane diterpenoid designated thysaspathone (2) were isolated from the liverwort Thysananthus spathulistipus, while Radula appressa produced radulannin A (3), radulannin L (4), 2-geranyl-3,5-dihydroxybibenzyl (5), 2(S)-2-methyl-2-(4-methyl-3-pentenyl)-7-hydroxy-5-(2-phenylethyl) chromene (o-cannabichromene) (6), 6-hydroxy-4-(2-phenylethyl) benzofuran (7), and o-cannabicyclol (8). All of the isolated compounds inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the greatest inhibition was attributed to compound 5, with an IC50 value of 4.5 microM.