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2017年毛细管电泳技术年度回顾 总被引:1,自引:0,他引:1
本文为2017年毛细管电泳(CE)技术的年度回顾。归纳总结了ISI Web of Science中检索到的2017年度发表的与CE技术相关的论文,从药物分析、医学及临床检验、食品安全检验、环境监测、CE-质谱联用技术、手性分析、生物分子7个方面进行分类说明。简要介绍了2017年涉及CE技术的国际会议7个、国内会议2个以及各会议的研究报告情况。最后,介绍了2017年度中国分析测试协会科学技术奖(CAIA奖)一等奖"毛细管电泳准确定量和精准筛选的新方法研究及应用"内容。 相似文献
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该文为2020年毛细管电泳(capillary electrophoresis, CE)技术年度回顾。归纳总结了以“capillary electrophoresis-mass spectrometry”或“capillary isoelectric focusing”或“micellar electrokinetic chromatography”或“capillary electrophoresis”为关键词在ISI Web of Science数据库中进行主题检索得到的2020年CE技术相关研究论文222篇,以及中文期刊《分析化学》和《色谱》中CE技术相关的研究论文37篇。对2020年影响因子(IF)≥5.0的Analytical Chemistry, Food Chemistry, Analytica Chimica Acta和Talanta等13本期刊的38篇文章报道的科研工作作了逐一介绍;对IF<5.0的期刊中CE技术报道较为集中的Journal of Chromatography A和Electrophoresis两本分析化学类期刊发表40篇文章中的代表性内容作了综合介绍;对重要的中文期刊《分析化学》出版的“核酸适配体专刊”和《色谱》出版的2期CE技术专刊所收录的37篇文章中的工作作了总体介绍。总体来说,2020年CE技术发展趋势仍以毛细管电泳-质谱(CE-MS)的新方法和新应用最为突出,主要集中在CE-MS与电化学检测、固相萃取以及多种毛细管电泳模式的联用方面,CE-MS接口相关的报道较前几年有所减少;常规CE技术则以胶束电动毛细管色谱(MEKC)在复杂样本分析、浓缩富集应用为主,尤其在食品和药品等复杂基质样本分析方面的报道较为集中;此外,我国CE相关领域专家学者的科研成果涵盖了CE在生命科学、临床医学、医药研发、环境科学、天然产物、食品分析等领域的应用,代表了国内CE科研应用水平和现状。 相似文献
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该文为2022年毛细管电泳(capillary electrophoresis, CE)技术年度回顾。归纳总结了以“capillary electrophoresis-mass spectrometry”或“capillary isoelectric focusing”或“micellar electrokinetic chromatography”或“capillary electrophoresis”为关键词在ISI Web of Science数据库中进行主题检索(排除“capillary electrochromatography”“microchip”“microfluidic”“capillary monolithic column”)得到的2022年CE技术相关研究论文881篇。重点介绍了影响因子(IF)大于10.0的Lancet Global Health,ACS Central Science,Microbiome,Trends in Food Science&Technology,TrAC-Trends in Analytical Chemistry,Jou... 相似文献
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A multiplex single nucleotide polymorphism genotyping method using ligase‐based mismatch discrimination and CE‐SSCP 下载免费PDF全文
Woong Choi Gi Won Shin Hee Sung Hwang Seung Pil Pack Gyu Yong Jung Gyoo Yeol Jung 《Electrophoresis》2014,35(8):1196-1203
Accuracy, simplicity, and cost‐effectiveness are the most important criteria for a genotyping method for SNPs compatible with clinical use. One method developed for SNP genotyping, ligase‐based discrimination, is considered the simplest for clinical diagnosis. However, multiplex assays using this method are limited by the detection method. Although CE has been introduced as an alternative to error prone microarray‐based detection, the design process and multiplex assay procedure are complicated because of the DNA size‐dependent separation principle. In this study, we developed a simple and accurate multiplex genotyping method using reaction condition‐optimized ligation and high‐resolution CE‐based SSCP. With this high‐resolution CE‐SSCP system, we are able to use similar‐sized probes, thereby eliminating the complex probe design step and simplifying the optimization process. We found that this method could accurately discriminate single‐base mismatches in SNPs of the tp53 gene, used as targets for multiplex detection. 相似文献
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微流控芯片毛细管电泳在蛋白质分离分析中的应用研究进展 总被引:1,自引:1,他引:0
重点介绍了近年来国内外在微流控芯片毛细管电泳法用于蛋白质分离分析方面的研究进展。按照分离模式的不同,综述了各种应用于蛋白质分离的微流控芯片毛细管电泳系统,讨论了抑制芯片中的蛋白吸附的各种方法,并展望了芯片毛细管电泳系统在蛋白质分离领域的发展前景。引用文献47篇。 相似文献
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Flow manipulation in sweeping microchip capillary electrophoresis (CE) is complicated by the free liquid communication between channels at the intersection, especially when the electroosmotic flows are mismatched in the main channel. Sweeping in traditional CE with cationic micelles is an effective way to concentrate anionic analytes. However, it is a challenge to transfer this method onto microchip CE because the dynamic coating process on capillary walls by cationic surfactants is interrupted when the sample solution free of surfactants is introduced into the microchip channels. This situation presents a difficulty in the sample loading, injection and dispensing processes. By adding surfactant at a concentration around the critical micelle concentration and by properly designing the voltage configuration, the flows in a microchip were effectively manipulated and this sweeping method was successfully moved to microchip CE using tetradecyltrimethylammonium bromide (TTAB). The sweeping effect of cationic surfactant in the sample solution was discussed theoretically and studied experimentally in traditional CE. The flows in a microchip were monitored with fluorescence imaging, and the injection and sweeping processes were studied by locating the detection point along the separation channel. A detection enhancement of up to 500-fold was achieved for 5-carboxyfluorescein. 相似文献
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毛细管电泳(CE)在新药研发领域显示着重要的应用前景。CE使用水溶液介质作为实验体系,保证了药物筛选在类似于生命介质的环境中进行,优于其他传统体外仪器筛选方法。除了维持被筛选分子和作用对象的生物活性外,CE筛选过程着重突出配体与受体之间的相互作用。毛细管电泳药物筛选瞄准与药理学理论相关的重要参数,如结合常数Kb 、结合速率常数Kon 和解离速率常数Koff ,有利于模拟并预测机体内靶标与药物之间的相互作用过程。该文回顾了毛细管电泳进行药物筛选的历史,评述了毛细管电泳药物筛选方法所依据的理论和相对成熟的各种常用方法,并抽取了部分典型实例以及相关技术进行说明,对以亲和毛细管电泳、动力学毛细管电泳为手段的药物筛选方法进行了介绍,包括分子和细胞层次的药物筛选,以及针对不同类型的候选药物的研究工作都有提及。毛细管电泳与多种技术的联用,包括与质谱以及化学发光等联用发挥了更大的效能。联用方法还应用于中药有效成分的筛选。毛细管电泳在DNA编码化合物库筛选中将有良好应用前景。馏分收集的发展为筛选药物提供了广阔前景,它配合指数富集配体系统进化技术为毛细管电泳药物筛选提供了更多可能。总之,毛细管电泳多样可选的药物筛选方法和技术将为新概念的药物筛选与药物评价提供有力支撑。 相似文献
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Aptamers, which are nucleic acid oligonucleotides that can bind targets with high affinity and specificity, have been widely applied as affinity probes in capillary electrophoresis (CE). Due to relative weak interaction between aptamers and small molecules, the application of aptamer-based CE is still limited in certain compounds. A new strategy that is based on the aptamer structure-switch concept was designed for small molecule detection by a novel CE method. A carboxyfluorescein (fluorescein amidite, FAM) label DNA aptamer was first incubated with partial complementary strand (CS), and then the free aptamer and the aptamer-CS duplex were well separated and determined by metal cation mediated CE/laser-induced fluorescence. When the target was introduced into the incubated sample, the hybridized form was destabilized, resulting in the changes of the fluorescence intensities of the free aptamer and the aptamer-CS duplex. The length of CS was investigated and 12 mer CS showed the best sensitivity for the detection of cocaine. The presented CE-LIF method, which combines the separation power of CE with the specificity of interactions occurring between target, aptamer, and CS, could be a universal detection strategy for other aptamer-specified small molecules. 相似文献
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Multiphoton excitation is a relatively old concept in quantum optics. But using multiphoton excitation fluorescence (MPEF) for bioanalysis is still in its infancy. Recently, MPEF has been introduced into the microseparation field, particularly CE, as a novel detection method. In this paper, MPEF detection for CE is reviewed, including MPEF fundamentals, approaches to achieving MPEF, detector configurations and applications in biological and environmental analyses. Emphasis will be placed on some recent advances of CE-MPEF in our laboratory. Challenges and future prospects are also discussed. 相似文献
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Capillary electrophoresis (CE) is a high-resolution technique for the separation of complex biological and chemical mixtures. CE continues to emerge as a powerful tool in the determination of lipids. Here we review the analytical potential of CE for the determination of a wide range of lipids. The different classes of lipids are introduced, and the different modes of CE and optimization methods for the separation of lipids are described. The advantages and disadvantages of the different modes of CE compared to traditional methods like gas chromatography (GC) and liquid chromatography (LC) in the determination of lipids are discussed. Finally, the potential of CE in the determination of lipids in the future is illustrated. 相似文献
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Capillary electrophoresis (CE) was used to separate polycyclic aromatic sulfur heterocycles (PASHs), a class of compounds
that occurs in fossil fuels and refined products of petroleum. An electric charge was introduced into the compounds through
methylation or phenylation of the sulfur atom. Separations of standard PASHs that are expected to be present in industrially
desulfurized fuels showed that CE possessed a higher resolution than reversed phase liquid chromatography. The CE method can
separate all the monomethylbenzothiophenes; this is not achieved in capillary gas chromatography. A linear relationship was
found between migration time and the calculated volume of the compounds. The PASHs in deeply desulfurized diesel were separated
after preconcentration, and the electropherogram was compared with the chromatograms from GC and HPLC. Finally, derivatized
PASHs are often enantiomeric and the enantiomers can be separated if a suitable cyclodextrin is added to the running buffer. 相似文献