LC-MS/MS测定小鼠肾脏中甘草次酸的含量

建立LC-MS/MS测定小鼠肾脏中甘草次酸含量的方法.本法以原儿茶酸为内标物质,采用多反应监测模式,对肾脏中甘草次酸进行定量研究.结果所建方法灵敏、精确、快速、线性范围宽,可用于甘草次酸与雄黄联合用药小鼠肾脏中甘草次酸的含量测定.     

微透析-HPLC测定甘草次酸对雄黄染毒小鼠海马细胞外液中Cys和Hcy含量的影响

建立微透析-HPLC测定小鼠海马细胞外液中Cys和Hcy含量的方法 ,探讨甘草次酸对雄黄染毒小鼠海马细胞外液中Cys和Hcy含量的影响.结果表明,所建方法准确、可靠,具有连续活体取样、实时动态检测的优势.此外,甘草次酸对雄黄引起的小鼠海马细胞外液中Cys和Hcy含量的降低有保护作用.     

高效液相色谱法分析测定甘草、甘草酸制品及中成药中β－型甘草次酸的含量

 报道了应用高效液相色谱法，ＳＰＣ＿（１８）色谱柱，甲醇－水－冰醋酸（７９：２０：１，Ｖ／Ｖ）流动相，ＵＶ２５４ｎｍ，以标准甘草次酸为外标，测定了甘草、甘草酸制品及中成药中甘草次酸的含量。本法适用于测定不同中成药及甘草酸制品中的甘草次酸的含量，方法简便，快速准确。     

高效液相色谱法分析测定甘草、甘草酸制品及中成药中β－型甘草次酸的含量

报道了应用高效液相色谱法，ＳＰＣ＿（１８）色谱柱，甲醇－水－冰醋酸（７９：２０：１，Ｖ／Ｖ）流动相，ＵＶ２５４ｎｍ，以标准甘草次酸为外标，测定了甘草、甘草酸制品及中成药中甘草次酸的含量。本法适用于测定不同中成药及甘草酸制品中的甘草次酸的含量，方法简便，快速准确。     

HPLC研究甘草次酸对大鼠槲皮素血浆浓度及肝脏分布的影响

建立了大鼠血浆和肝脏槲皮素的高效液相色谱测定方法,并用于探索甘草次酸对大鼠槲皮素血浆浓度及肝脏分布的影响。采用ODS-SP柱,流动相为甲醇-0.4%H3PO4溶液,检测波长为370 nm,流速为1.0 mL/min。结果显示血浆和肝脏槲皮素测定的线性范围分别是0.050～10.0μg/mL和0.125～0.625μg/g。血浆和肝脏测定回收率分别是92%～101%和80%～96%,检测限分别是0.048μg/mL和0.011μg/g。大鼠口服槲皮素后在血浆未能检测到游离槲皮素,低浓度以及高浓度合药组中槲皮素浓度(水解后)高于单药组。甘草次酸对槲皮素肝脏分布没有显著影响。     

高效毛细管电泳法测定银翘解毒片中的氯原酸、甘草酸和甘草次酸

建立了同时测定银翘解每片中氯原酸、甘草酸和甘草次酸的高效毛细管电泳法,电解缓冲液为20mmol／L磷酸二氢钠和5mmol／L硼砂的混合溶液（pH7.0）。方法简便快速,具有良好的精密度、回收率和线性关系,并测定了很翘解毒片中3组分的含量。     

高脂与维生素D缺乏饮食诱导的2型糖尿病小鼠血清和肝脏代谢组学研究

采用高脂与维生素D缺乏(VDD)饮食长期(24周)喂养小鼠,诱导其形成2型糖尿病(T2DM),通过小鼠血清和肝脏代谢组学分析探究了T2DM发生、发展的代谢物和代谢通路变化机制.实验收集小鼠血清和肝脏样品,通过气相色谱-质谱联用技术和硅烷化衍生方法分析得到血清和肝脏代谢轮廓;利用正交偏最小二乘判别分析和非参数检验筛选血清和肝脏代谢组中具有显著性差异的代谢标志物,发现血清样品中乳酸、丙氨酸、甘油、苏氨酸和葡萄糖含量在高脂+VDD小鼠中显著升高,肝脏样品中乳酸、核糖、果糖、葡萄糖、油酸和棕榈酸含量在高脂+VDD小鼠中显著升高.本文还进行了血清和肝脏代谢轮廓整体分析和代谢通路探索,发现高脂+VDD小鼠中三羧酸循环、糖异生、氨基酸以及脂质代谢通量均显著增强,这些代谢路径相互影响共同促进T2DM的发生和发展.本文通过饮食诱导小鼠形成T2DM,得到血清和肝脏代谢物及其代谢通路的变化关系,可为T2DM诊断提供参考信息.     

甘草次酸衍生物的合成

豆科植物甘草中含有大量甘草次酸, 甘草的抗炎症, 抗溃疡以及抗变态反应等功效, 主要归用于甘草中所含的甘草甜素, 甘草次酸及其衍生物, 为了寻找疗效高, 副作用低的新药成分, 本文分别以甘草次酸和甘草次酸甲酯为原料, 合成了还原产物和酯化产物十二个衍生物。     

甘草次酸衍生物的合成

豆科植物甘草中含有大量甘草次酸(Glycyrrhetinic Acid)。近代药理实验表明,甘草的抗炎症、抗溃疡以及抗变态反应等功效,主要归因于甘草中所含的甘草甜素,甘草次酸及其衍生物。多年来,国内外学者对甘草次酸     

赖氨酸在甘草次酸弹性囊泡形成过程中的作用机制

制备和评价含赖氨酸的甘草次酸弹性囊泡, 并考察赖氨酸在囊泡形成过程中的作用机制. 在水合介质中加入赖氨酸, 利用薄膜-高压均质法制备甘草次酸弹性囊泡. 并合成了甘草次酸赖氨酸盐及其弹性囊泡作为对比制剂. 通过对粒径、zeta电位、包封率、相转变温度、变形性和体外经皮渗透性的测试, 考察赖氨酸在甘草次酸弹性囊泡中的存在形式及作用. 结果显示加入赖氨酸后, 甘草次酸弹性囊泡的粒径略有降低, 膜相转变温度降低, 包封率和囊泡变形性显著提高, 载药量提高近30倍(1.5 mg·mL-1), 并显著高于其赖氨酸盐所形成囊泡的载药量和弹性. 此外, 赖氨酸的加入使弹性囊泡的变形能力增加, 8 h累积透过量和皮肤驻留量分别提高4.3倍和9.2倍. 表明赖氨酸与甘草次酸形成离子缔合物, 促进甘草次酸参与膜的形成, 使膜的流动性增加, 赖氨酸与弹性囊泡对提高囊泡载药量起协同作用.     

Neutral diterpenoids of oleoresins of five species of conifers of Transcarpathia

The compositions of the neutral diterpenoids of the oleoresins of five species of conifers growing in the Transcarpathia have been studied. It has been found that the oleoresins ofAbies alba M.,Larix decidua M., andPicea excelsa L. contain more than 50% of neutral diterpenoids. The group and qualitative compositions of the oxygen-containing diterpenoids have been determined. In the oleoresins ofAbies alba,Picea excelsa, andPicea abies tertiary alcohols — cis-abienol and isocembrol — predominate, while inLarix decidua the main component is the hydroxy ester larixyl acetate. Primary alcohols related to the resin acids have been found in all the oleoresins investigated. Features of the distribution of diterpenoids according to the species of conifers have been revealed. The results obtained are necessary for the chemotaxonomy of conifers of the family Pinceae.Novosibirsk Institute of Organic Chemistry, Siberian Branch, Academy of Sciences of the USSR. Translated from Khimiya Prirodynkh Soedinenii, No. 6, pp. 812–816, November–December, 1988.     

利用手持技术改进测定乙醇分子结构实验

利用压强传感器代替排水集气法,改进测定乙醇分子结构实验的仪器装置,并探索最佳反应条件。另外,设计两个空白实验,结合压强变化曲线对实验误差进行相关讨论。     

光谱仪测试范围的扩展

由于石化行业的生产需要，其材质的使用具有多样性和广泛性，经常会出现顾客委托的测试样品的一个或几个元素跨越光谱仪现有测试程序测量范围的情况。本法通过对光谱仪测试原理的认识，根据光谱仪的测试能力及标样的采集，实现了一个或几个元素测量范围的扩展，并对其测量的影响因素进行了研究。     

Effect of the Method of Modification of Zeolite X on Selectivity of Catalytic Methylation of Toluene

Theoretical and Experimental Chemistry - The effect of the method of modification of catalysts based on zeolite X by cesium and magnesium cations (ion exchange and impregnation) on the selectivity...     

Influence of viscosity of medium on processes of oxygen quenching of excited states of mesoporphyrin

The oxygen quenching rate constants for singlet and triplet excited states of the dimethyl ester of mesoporphyrin IX increase with decreasing viscosity of the medium and reach a maximum at a viscosity of approximately 0.4 mPa·sec, after which the rate constants begin to decrease. The drop in rate constant with increasing fluidity of the medium may be related to a nonequilibrium character of the elementary act in the interaction of the reactants in the solvent cage. In viscous media such as isopropyl alcohol, isobutyl alcohol, and isoamyl alcohol, the reaction radius is greater than the sum of the radii of the reacting particles. The long-range character of these processes is due to the relatively weak dependence of the quenching probability on distance.Translated from Teoreticheskaya i Éksperimental'naya Khimiya, Vol. 25, No. 2, pp. 161–167, March–April, 1989.     

Dichroism of absorption and polarization of phosphorescence of molecules of the closed form of nitrobenzospirothiopyran

The spectral-polarization characteristics of absorption and phosphorescence of molecules of the initial form of nitro-substituted indolinospirobenzothiopyran were studied in oriented polyethylene films and in solutions with different polarity. An oscillator model of the electron transitions responsible for the formation of absorption and luminescence spectra was suggested. It was established that the principal differences in the spectral and photophysical properties of the compound studied and its oxygen-containing analog are associated with the fact that the electronegativity of the S atom is lower than that of the O atom. Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 6, pp. 1143–1146, June, 1997.     

Synthesis of tetrabenzoporphins of the basis of isoindoline

Chemistry of Heterocyclic Compounds -