(PtI6-2RDG)n缔合纳米微粒体系的共振散射、荧光猝灭和减色效应研究

在0.02mol/L HCl介质中，罗丹明6G（RDG）分别在530nm和550nm处有一个吸收峰和荧光峰，PtI6^2-与RDG^ 主要通过静电引力形成疏水性的PtI6-2RDG缔合物分子。PtI6-2RDG分子间存在较强的分子和和疏水作用力而生成（PtI6-2RDG)n缔合纳米微粒，其粒径为40nm，在400nm、470nm和590nm产生3个共振散射，其中400nm和590nm处的2个峰为其特征共振散射峰，550nm荧光峰和530nm吸收峰的降低是由于纳米微粒形成后，只有裹露在（PtI6-2RDG)n纳米微粒界面的RDG荧光分子才能吸收激发光子跃迁到激发态，进而返回基态产生荧光，而体体相的RDG荧光分子无法与激发光作用产生荧光，即与激发光作用的RDG分子数大为降低。当该纳米微粒体系加入乙醇后，由于乙醇致使（PtI6-2RDG)n纳米微粒分解为PtI6-2RDG分子，体系的红紫色和共振散射峰消失，吸收峰和荧光峰恢复，研究结果表明，红紫色（PtI6-2RDG)n纳米微粒的形成是其共振散射增强、荧光猝灭、减色效应和产生特征共振散射峰的根本原因。     

四苯硼钠-甲苯胺蓝缔合物纳米微粒体系减色效应研究

在PH4.0醋酸-醋酸钠介质中，甲苯胺蓝在600nm处有一个吸收峰，随着四苯硼钠浓度的增大甲苯胺蓝在600nm处吸收峰降低，颜色减弱，这是由于甲苯胺蓝-四苯硼钠缔合物分子间存在较强的疏水作用及分子间作用力，聚集形成纳米微粒所致，甲苯胺蓝-四苯硼钠纳米微粒体系亦在600mm处有1个吸收峰，在400mm、470mm和580mm处产生3共振散射峰，其中400mm和580mm为甲苯胺蓝-四苯硼钠复合纳米微粒产生的特征共振散射峰，这也表明有纳米微粒存在，丙酮浓度的影响实验结果等表明，纳米微粒的形成是产生其减色效应的原因。     

西替利嗪-碘铂酸缔合微粒体系的吸收光谱研究及分析应用

Pt(IV)与I-形成[PtI6]2-,[PtI6]2-和盐酸西替利嗪(CTRZ)通过静电引力作用形成疏水性的(PtI6—CTRZ)缔合物分子.由于(PtI6-CTRZ)缔合物分子间存在较强的分子间作用力和疏水作用力而生成紫红色(CTRZ—PtI6)n缔合微粒,在310、400、610nm处产生3个共振散射峰;在350～740nm波长范围的吸光度值均增大.在选定条件下,CTRZ浓度在0～10μg/mL范围内与A580nm成正比,摩尔吸光系数ε580nm为1.30×104L/(mol·cm).实验结果表明,(CTRZ—PtI6)n缔合微粒的形成是导致同步散射信号增强的根本原因,而纳米纳米微粒的颜色是共振散射所致.     

(阳离子表面活性剂-I3)n缔合微粒体系的光谱特性及分析应用

在0.01 mol/L HCl介质中,I-3在350 nm处有一吸收峰;当十六烷基三甲基溴化铵(CTMAB)与I-3共存时体系呈红紫色,在550 nm处产生一新的吸收峰.CTMAB浓度CCTMAB在0.0～7.0×10-5 mol/L范围内符合比耳定律,回归方程为A550 nm =0.989×104 CCTMAB+0.0138,相关系数R为0.999 5,摩尔吸光系数ε为1.06×104 L/(mol·cm),据此建立了一种测定阳离子表面活性剂含量的分光光度新方法,并用于合成样品和新洁尔净样品中阳离子表面活性剂测定.共振散射光谱研究表明,CTMAB+与I-3可通过静电引力作用形成疏水性的CTMA-I3缔合物分子,并进一步聚集形成稳定的 (CTMA-I3)n缔合微粒.由于该缔合微粒在580 nm处产生共振散射效应,故体系呈红紫色.     

小檗碱-I－3缔合微粒体系的光度分析应用

在稀HCl介质中,I-3在340 nm处有一吸收峰;当小檗碱(BB)与I-3共存时体系呈橙黄色,在580 nm处产生一共振散射峰.以试剂作参比,该缔合微粒体系在530 nm产生一吸收峰,BB浓度在0～7.0×10-5mol/L范围内与A530 nm呈线性,据此建立了一种测定小檗碱含量的分光光度新方法,并用于针剂样品中小檗碱测定,结果满意.同步散射光谱研究表明,BB+与I-3可通过静电引力作用形成疏水性的(I3-BB)缔合物分子,并进一步聚集形成稳定的(I3-BB)n缔合纳米微粒.由于该缔合纳米微粒在580 nm处产生共振散射效应,故体系呈橙黄色.     

小檗碱-Ⅰ3^-缔合微粒体系的光度分析应用

在稀HCl介质中,I-3在340 nm处有一吸收峰;当小檗碱(BB)与I-3共存时体系呈橙黄色,在580 nm处产生一共振散射峰.以试剂作参比,该缔合微粒体系在530 nm产生一吸收峰,BB浓度在0～7.0×10-5mol/L范围内与A530 nm呈线性,据此建立了一种测定小檗碱含量的分光光度新方法,并用于针剂样品中小檗碱测定,结果满意.同步散射光谱研究表明,BB+与I-3可通过静电引力作用形成疏水性的(I3-BB)缔合物分子,并进一步聚集形成稳定的(I3-BB)n缔合纳米微粒.由于该缔合纳米微粒在580 nm处产生共振散射效应,故体系呈橙黄色.     

小檗碱-I_3~-缔合微粒体系的光度分析应用

在稀HCl介质中,I_3~-在340 nm处有一吸收峰;当小檗碱(BB)与I_3~-共存时体系呈橙黄色,在580 nm处产生一共振散射峰。以试剂作参比,该缔舍微粒体系在530 nm产生一吸收峰,BB浓度在0～7.0×10~(-5)mol/L范围内与A_(530nm)呈线性,据此建立了一种测定小檗碱含量的分光光度新方法,并用于针剂样品中小檗碱测定,结果满意。同步散射光谱研究表明,BB~+与I_3~-可通过静电引力作用形成疏水性的(I_3-BB)缔合物分子,并进一步聚集形成稳定的(I_3-BB)_n缔合纳米微粒。由于该缔合纳米微粒在580 nm处产生共振散射效应,故体系呈橙黄色。     

液相卤化银纳米微粒的界面荧光和共振散射光谱特性

液相卤化银纳米微粒的共振散射光谱和发射光谱表明，AgCl和AgBr纳米微粒均在330，400，470和680nm处产生4个共振散射峰，在340，400和470nm处产生三个荧光峰．Ad纳米微粒在340，400，437，470和680nm处产生5个共振散射峰；除在340，400和470nm处产生3个荧光峰外，在434nm处有一最强的荧光峰．卤化银纳米微粒体系的浓度对共振散射信号的影响与浓度对荧光强度的影响一致，Aga，AgBr和AgI体系的共振散射光信号强度分别约为荧光信号的110，130和80倍，即荧光与共振散射之间存在相关性．提出了液相AgX纳米微粒荧光产生机理，解释了荧光与共振散射之间存在相关性的原因．     

染料分子对硫纳米微粒共振散射光谱的影响

在聚丙烯酰胺存在下液相硫纳米微粒在 470nm处产生 1个强共振散射峰 ;在可见光范围内无吸收峰且吸收值较小。硫微粒质量浓度在 0 0 5～ 1 0mg/L范围内与I4 70nm间有良好线性关系。研究了乙醇、丙酮 ,以及溴酚蓝、溴甲基紫、结晶紫、亮绿等有机染料对硫纳米微粒共振散射的影响。结果发现 ,染料分子吸收是产生共振散射峰的一个重要原因 ;随着染料分子非辐射吸收值的增大 ,硫纳米微粒共振散射光强度降低。实验证明 ,溴酚蓝浓度在 0～ 1 0× 10 -5mol/L范围内 ,在溴酚蓝最大吸收波长 5 90nm处的ΔI590nm与溴酚蓝浓度呈线性关系。     

盐酸小檗碱-十二烷基苯磺酸钠缔合微粒体系的共振散射光谱研究及其分析应用

在pH 4 .80NaAc HAc缓冲介质中 ,盐酸小檗碱 (BH)与十二烷基苯磺酸钠 (DBS)由于静电引力和疏水作用力形成缔合微粒 ,在 4 70nm处有一共振散射峰。随着DBS浓度增大 ,该峰急剧增强 ,即存在缔合微粒的散射光增强效应 ;345nm处的吸光度减弱 ,即存在缔合微粒的减色效应。研究了共振散射光谱测定BH的影响因素 ,提出了测定 (0 .35～ 4 .4 )× 10 -5mol/L盐酸小檗碱的共振散射光谱新方法 ,用于复方黄连素片剂和针剂样品的测定 ,结果满意     

Spectral properties of the association nanoparticle system of ciprofloxacin-phloxine and its application to fluorescence analysis.

There is a fluorescence peak at 570 nm, and a maximum absorption peak at 560 nm for phloxine (PHLO) in a pH 7 water solution. Under these conditions, the ciprofloxacin cation (CPFX+) and PHLO- combine into hydrophobic CPFX-PHLO association molecule by means of static gravitation. There are stronger van der Waals forces and hydrophobic forces among the CPFX-PHLO molecules. Thus, they aggregate automatically to the (CPFX-PHLO)n association nanoparticle in red-violet color. That was characterized by scan electron microscopy (SEM), hyperfiltration and dialysis tests. In 0.04 M HCl, the red-violet nanoparticles exhibited a Rayleigh scattering peak at 470 nm, a resonance scattering peak at 580 nm, a maximum absorption wavelength at 565 nm, and a fluorescence peak at 450 nm. The fluorescence analytical conditions of CPFX have been considered. The CPFX concentration in the range of 1.0 x 10(-6)-4.0 x 10(-5) M is linear to the fluorescence intensity, F450nm. The detection limit was achieved at 4.0 x 10(-7) M CPFX. The CPFX in real samples was determined with satisfactory results.     

罗丹明B-PdI2-4缔合纳米粒子体系的极谱猝灭效应

缔合纳米微粒;共振散射;罗丹明B-PdI2-4缔合纳米粒子体系的极谱猝灭效应     

HAS-硅钨杂多酸缔合纳米微粒体系的荧光猝灭

人血清白蛋白;瑞利散射;HAS-硅钨杂多酸缔合纳米微粒体系的荧光猝灭     

罗丹明6G缔合微粒荧光猝灭法测定痕量碘酸根

研究发现在0.01mol/LHCl-8.0×10-4mol/LKI介质中,罗丹明6G(RhG)在550nm处有1个荧光峰.当有IO-3,I-3与RhG形成缔合微粒,550nm处荧光峰猝灭,在320、400、6103存在时,IO-3与过量的I-反应生成I-nm处有3个共振散射峰,在470nm处有1个同步散射峰.碘酸根浓度在2.0～100×10-7mol/L范围内与荧光猝灭强度成线性关系.据此建立了一个测定食盐中IO-3的荧光猝灭分析法.光谱研究结果表明,(RhG-I3)n缔合微粒和界面的形成是导致体系荧光猝灭的根本原因.     

Change Color Effect and Spectral Properties of Gold Nanoparticle-cationic Surfactants System

The change color effect of gold nanoparticle solutions was studied by means of resonance scattering and absorption spectrometry and scan electron microscopy. The red Au nanoparticles with a size of 10 nm exhibit a resonance absorption peak and a resonance scattering peak all at 525 nm. After some inorganic electrolyte was added to a red Au nanoparticles solution, the color of the solution became blue and the absorbance at 600-700 nm was significantly increased. The ratio of the concentration of rnonovalent cations, at which the resonance scattering of the system at 525 nm is maximal to that of divalent cations, is in the range of 100 : 1--100 ; 1. 8. It is in good agreement with the Schulze-Hardy rule of the coagulation value of electrolyte. After adding some cationic surfactants to the above solution, the color of the solution is in deep blue, with two resonance absorption peaks at 550 and 680 nm, and a greatly enhanced resonance scattering peak at 525 nm. The experiments demonstrate that the stronger the hydrophobicity of the cationic surfactant is, the stronger the change color effect of the Au nanoparticle solution promoted by cationic surfactant is. The change color effect of Au nanoparticle solution is resulted from the increased diameter of Au nanoparticles, and the changes of resonance absorotion DeaR and resonance scattering.     

Absorption and Rayleigh scattering and resonance Rayleigh scattering spectra of [HgX<Subscript>2</Subscript>]<Subscript>n</Subscript> nanoparticles

The composition and existing species ot the reaction production ot Hg ana X (X= Cl, Br and I) under different conditions, and their absorption, Rayleigh scattering (RS) and resonance Rayleigh scattering (RRS) spectra have been studied. The results show that the products exist in the form of nanoparticles as [HgX2]n aggregates under suitable conditions, and their average diameters increase with the increase of X- diameters. The diameters of [HgCI2]n, [HgBr2]n and [Hgl2]n are less than 4 nm, equal to 9 nm and 70 nm respectively. There are bathchromic shifts gradually with the increase of X- diameters in their absorption spectra. The absorption bands of [HgCI2]n and [HgBr2]n locate at ultraviolet region. However, the absorption band of [Hgl2]n is obvious in visible light region. Among three particles, only [Hgl2]n exhibits a strong RRS and its scattering peak is at 580 nm. The main reasons leading to the enhancement of resonance scattering are the large size of nanoparticle, the formation of the interface     

金纳米粒子-荧光素体系的光谱特性

纳米粒子具有量子尺寸效应和表面效应等许多特有的性质 [1] ,在光吸收、医药及新材料等方面具有广阔的应用前景 .纳米粒子具有较高的比表面能且带有电荷 ,当光子与其接近时 ,实际上是光子与纳米粒子的界面电子发生了作用 [2 ,3 ] .基于此建立的共振散射 (RS)光谱技术已成为一种高灵敏度和高选择性的分析技术 ,是研究生物化学和液相纳米粒子特性的良好手段 [4～ 9] .我们 [2 ,3 ] 研究发现 ,较大粒径纳米粒子和界面的形成是导致散射光增强的根本原因 ;金、银等液相纳米粒子产生 RS效应和 RS峰等 .荧光猝灭 (FQ)效应已用于分析化学和蛋白…     

Resonance scattering spectral analysis of chlorides based on the formation of (AgCl)n(Ag)s nanoparticle

A novel resonance scattering spectral method has been proposed for the determination of trace amounts of chlorides in the range of 2 x 10(-7)-8 x 10(-6) mol/l. It was based on the photochemical reaction system of AgNO3-NaCl-sodium oxalic to form the (AgCl)nucleus (n)(Ag)shell (s) nanoparticle. There is a strongest resonance scattering peak at 470 nm and a maximum absorption peak at 425 nm. The concentration of chlorides is proportional to the intensity of resonance scattering at 470 nm. The nonlinear resonance scattering peaks of the nanoparticle system have been also considered, according to the theory of the interaction between the surface electron of nanoparticle and the incidence photon.