利用小尺寸电荷耦合器件实现数字全息高分辨成像

为了在降低数字全息显微成像系统成本的同时实现高分辨成像,对像面数字全息显微术的记录与再现过程进行了理论分析,结合系统的点扩散函数,对该系统的横向分辨率进行了分析. 得到了如下结论：像面数字全息显微系统的横向分辨率对电荷耦合器件（CCD）光敏面尺寸变化不敏感;对于常见的CCD 器件,其像元尺寸的变化对该系统的横向分辨率影响甚微. 此外,对像面数字全息显微系统的成像特点进行了分析,结果表明：利用像面数字全息系统可以实现物体信息的完整记录与再现,其成像分辨率及像质优于预放大数字全息系统. 利用搭建的数字全息实验记录系统,从强度及位相两方面对理论分析结果进行了验证,实验结果表明了理论分析的正确性. 关键词： 像面数字全息术 横向分辨率 点扩散函数 小尺寸电荷耦合器件     

预放大数字全息显微系统的特点

为了提高预放大数字全息显微系统的成像质量,采用理论分析与实验验证相结合的方法,分别对采用平面及球面参考光记录的预放大数字全息显微系统进行了研究和比较。结果表明:在通常的实验条件下,系统的横向分辨力主要取决于显微物镜的成像分辨力;记录距离较小时,两种系统的横向分辨力均随着记录距离的增大略有降低;但当记录距离较大时,球面参考光预放大数字全息系统的横向分辨力降低得更为明显,即平面参考光预放大数字全息显微系统较为优越;在记录距离为0的情况下,即像面数字全息成像情况下,两种系统的再现像均具有最高的分辨力,在利用普通工业用传感器条件下,横向分辨力远超过了2.19 m,且像质较好。因此,尽可能减小全息图的记录距离,或者采用像面数字全息系统,可以有效提高数字全息系统的成像分辨力。     

优化的数字全息显微成像系统

基于预放大数字全息显微系统的全息图记录与再现过程及其点扩散函数的分析, 从成像分辨率、成像质量及实现的难易程度等方面对数字全息显微中六种常见记录光路系统的成像性能进行了对比研究. 结果表明, 像面数字全息术具有最高的成像分辨率及成像质量, 其成像分辨率与记录器件的光敏面尺寸无关, 该系统对信息的记录是完整的, 而且记录过程不必考虑物体被照亮区域的大小, 再现过程非常简单, 是优化的数字全息显微成像系统. 等波面弯曲的物参光像面数字全息术非常有利于位相解包裹及位相畸变补偿的正确进行, 该系统更适合于位相显微. 实验结果验证了理论分析的正确性. 关键词： 显微数字全息 预放大数字全息 像面数字全息 分辨率     

预放大数字全息系统的成像分辨率分析

基于菲涅耳衍射理论,详细推导了预放大数字全息系统的点扩散函数表达式,并由此分析了该成像系统的分辨率.通过分别对整个系统、显微物镜以及CCD的点扩散函数幅值的计算机模拟,讨论了显微物镜与CCD分辨率之间的匹配问题.指明了现有文献中一些含糊说法.结果表明:恰当选择记录参量,使MO的成像分辨率接近于其极限分辨率,同时还要使CCD在满足抽样条件与再现像分离条件的情况下,再现像分辨率等于或略高于MO的分辨率.     

高质量等曲率物参光像面数字全息显微系统

对等曲率物参光像面数字全息显微成像系统进行研究,分析了光路配置方法,推导了系统的点扩散函数,并由此指出了决定系统成像分辨率的因素及系统的成像特点,最后讨论了再现像光场一次位相畸变校正的方法.结果表明,等曲率物参光像面数字全息具有最大的信息容量;该系统的成像分辨率取决于显微物镜的数值孔径和CCD的像元大小,与CCD的光敏面尺寸无关;物体各点中通过显微物镜的所有频率成分均能被系统完全记录与再现,样品被照亮区域的大小对记录条件和再现像质没有影响;等曲率物参光像面数字全息系统是一种优化的全息记录与再现系统,利用该系统可以实现高质量成像.实验结果验证了理论分析的正确性.     

Simple and robust digital holography for high-resolution imaging

Based on the point spread function of holographic system,the lateral resolution of digital holographic imaging system without any pre-magnification is studied.The expression of resolution limitation of holographic imaging system is thus presented.We investigate the possibilities to improve the lateral resolution.The simple experimental setup with an off-axis arrangement is built.By using a U.S.Air Force(USAF)test target as microscopic object,the recorded holograms are reconstructed digitally based on the principle of Fresnel diffraction.The lateral resolution of 2.76 μm without any pre-magnification is demonstrated experimentally,which matches the theoretical prediction well.     

基于同态信号处理的数字全息广义线性重建算法研究

为了提高数字全息图的重建速度和精度,本文提出了一种基于同态信号处理的数字全息广义线性重建算法. 首先利用预放大数字全息显微系统并结合同态信号处理原理进行了理论分析,得到了广义线性重建算法的实现条件及重建步骤,并对该算法的优点进行了分析;然后利用计算机模拟和实验相结合的方法对理论分析进行了验证. 结果表明：数字全息广义线性重建算法不仅可以有效的消除全息图频谱中零级项的干扰,实现高精度再现,而且由于采用一个完整象限的固定区域滤波,避免了常规线性算法的手动滤波操作,极大地提高了重建速度,同时最大限度地保留了原始像中的高频成分,实现全息图的高分辨重建. 关键词： 数字全息显微术 同态信号处理 傅里叶变换 分辨率     

光束扫描超分辨数字全息

根据超分辨成像理论, 提出了一种基于光束扫描的超分辨数字全息记录与再现技术。用菲涅耳衍射理论证明了该技术可有效增大CCD探测器的等效尺寸和系统截止频率, 从而提高数字全息成像分辨率。计算机仿真验证了该方法的正确性与可行性。对光束扫描全息记录系统进行了详细描述, 对该数字全息术的再现方法进行了具体说明。对比合成孔径数字全息术, 该技术在全息记录中无需反复移动CCD探测器, 也不必反复调整参考光以避免出现欠采样。     

Quantitative assessment of lateral resolution improvement in digital holography

Controllable experimental features such as wavelength, camera’s specifications and reconstruction distance, determine the theoretical limit for lateral resolution in digital holography; however, due to the speckle noise associated to any coherent imaging system it is not possible to reach this theoretical limit. In this paper, a quantitative study the lateral resolution for digital holography under the effect the speckle noise is carried out. It is shown that by reducing the contrast of the speckle noise the resolution capabilities of digital holography are improved; the signal-to-noise ratio of the reconstructed holograms is the metric use to quantitatively assess the reached resolution in the holographic experiments.     

Study on the simplified phase-shifting digital holographic microscopy

In this paper, a new simplified technique for effectively eliminating the zero order and the conjugate virtual image in digital holographic microcopy, which makes use of two-step phase-shifting method of just recording two holograms and an intensity image of object wave, is proposed. Meanwhile, combined with the principle of making full use of spatial bandwidth of the CCD sensor by in-line lens-less Fourier holographic recording geometry, the theory and experimental methods to increase the resolution of the reconstructed image in digital holography by using phase-shifting technique are detailedly analyzed. At end, the validity and availability of this technique has been demonstrated through the off-axis and in-line Fourier transform recording geometry. The study provides some theoretical and experimental guidance for the design and operation of a digital holographic microscopy system.     

利用综合复用技术拓展数字全息显微系统中的记录视场

为了解决现有数字全息显微系统中高分辨率与大记录视场无法同时兼得的问题, 提出了一种在不牺牲分辨率的前提下拓展数字全息显微记录视场的方法. 该方法中运用了波长不同、偏振态不同的四路相互不相干的探测物光, 同时探测被测样品四个相邻的不同区域, 并使这四束探测物光分别与其相应的参考光相干, 在记录面上同时记录下含有被测样品不同区域信息的复合全息图. 将获得的复合全息图经过频谱变换和数字滤波, 分别重构出所记录区域的振幅和相位分布.最后通过图像拼接和图像融合技术, 可实现接近原记录视场四倍的大视场数字全息显微记录. 该方法在测量过程中无需移动记录装置、光源和被测样品, 单次曝光即可实现, 实验结果验证了本文所提方法的可行性. 关键词： 数字全息显微术 角分复用 偏振复用 波长复用     

Study on aberration suppressing methods in digital micro-holography

This paper discusses methods to eliminate and/or suppress aberrations in digital micro-holography (DMH) with specific examples for post-magnification DMH and lens-less DMH along with a phase subtraction approach. In post-magnification DMH, the reference wave interferes with the object wave to form a hologram, which is then magnified by a microscope objective (MO) and recorded by CCD. This compensates for the quadratic phase error usually present in the pre-magnification DMH and also it is easier to isolate the useful information in pre-processing. In this paper differences between post-magnification and pre-magnification DMH are compared and analyzed with the specimen of a phase grating with period 30 lines/mm and height 0.3 μm. A lens-less DMH is also described that can eliminate the quadratic error and most of wave-front errors of the projecting light. Finally, a phase subtraction method is demonstrated which can eliminate most of the system aberrations.     

球面参考光预放大数字全息成像系统分辨力

为了提高数字全息成像系统的成像分辨力,对以球面波作为参考光波的预放大数字全息成像系统的记录和再现过程进行了理论推导,得出了该系统的脉冲响应表达式,并由此分析了决定该系统成像分辨力的因素。在CCD成像分辨力高于显微物镜分辨力的情况下,通过对分辨力测试板进行实验,讨论了系统分辨力与记录距离的关系。结果表明:再现像的分辨力对记录距离不敏感;但在记录距离为零,即像面数字全息系统下,再现像具有更高的分辨力。实验结果与理论分析一致。     

Submicrometer digital in-line holographic microscopy at 32 nm with high-order harmonics

Soft-x-ray digital in-line microscopic holography is achieved using a fully coherent high-order harmonic source emitting at 32 nm. Combination of commercial-grade soft-x-ray optics and a back-illuminated CCD detector allows a compact and versatile holographic setup. Different experimental geometries have been tested by imaging calibrated 50 nm tips and 1 microm wires. Spatial resolution of 800 nm is measured with magnifications ranging from 30 to 110 and a numerical aperture around 0.01. Finally, the potentiality of three-dimensional numerical reconstruction from a single hologram acquisition is shown experimentally.     

基于滤波成像的大视角数字全息技术

为提高数字全息再现像视角,提出一种基于滤波成像的数字全息技术来实现大视角的三维物体面型测量.利用离轴像面数字全息技术,通过在4F相干图像处理系统的空间频谱面处放置可移动的低通滤波器,使满足CCD分辨率的物光波与参考光波干涉形成全息图,并控制低通滤波及成像区域分别记录不同谱段的子全息图.再现时,首先对子全息图进行数字傅里叶变换,重构对应频谱段,并对频谱段进行拼接形成完整的物光频谱|而后通过数字再现获得大视角的数字全息再现像.利用该方法测量了圆柱形表面（光滑的缝纫针）的三维形貌,并取得了较好的实验结果.     

Lensless Fourier holography for digital holographic interferometry on biological samples

Digital holography as a tool for highly sensitive, interferometric non-destructive testing has several advantages compared to holographic measurements based on conventional storage media like an all-digital processing and a direct access to the phase of the object wave. Experimental results of interferometric investigations of heart valve bio-prostheses with a setup for lensless Fourier holography are presented which demonstrate that this technique is applicable to such biological samples with their wet and unstable surfaces. Limitations on size and resolution of the reconstructed object caused by the properties of the CCD sensor are discussed.     

Immersion digital in-line holographic microscopy

Digital in-line holographic microscopy is a promising new tool for high resolution imaging. We demonstrate, by using latex beads, that a considerable increase in numerical aperture, and, therefore, resolution can be achieved if the space between a source and a CCD camera chip is filled with a high refractive index medium. The high refractive index medium implies a shorter effective wavelength so that submicrometer resolution can be obtained with laser light in the visible range.     

Direct-to-video holographic readout in quantum wells for three-dimensional imaging through turbid media

Customized photorefractive quantum-well devices have been developed for real-time video acquisition of coherence-gated, three-dimensional images in turbid media. Large-field-of-view holographic imaging with direct video capture is now possible. We have evaluated the role of intensity-limited device performance in Fourier-plane and image-plane holography in such devices and, using near-infrared light, have imaged through turbid phantoms of 13 mean free paths' scattering depth with 50-microm transverse and 60-microm depth resolution.     

数字全息技术的原理和应用

数字全息是随着现代计算机和CCD技术发展而产生的一种新的全息成像技术．文章主要介绍数字全息技术的基本原理，数字全息重建中的主要方法以及数字全息技术以其独特的优点在各个领域中的应用．     

基于涡旋光照明的暗场数字全息显微方法研究

提出了一种基于涡旋光照明的暗场数字全息显微方法. 从理论上阐述了涡旋光环形照明原理和暗场数字全息显微原理, 分析了涡旋光的准无衍射特性对成像的影响; 搭建了相应的数字全息显微成像系统, 采用690 nm的聚苯乙烯小球作为实验样品; 最后通过对小球明暗场下数字全息显微再现像的分析对比, 证明该方法可以有效地提高数字全息系统的分辨率, 同时增强了再现像的对比度. 关键词： 全息 暗场数字全息显微 涡旋光 分辨率