高质量等曲率物参光像面数字全息显微系统

对等曲率物参光像面数字全息显微成像系统进行研究,分析了光路配置方法,推导了系统的点扩散函数,并由此指出了决定系统成像分辨率的因素及系统的成像特点,最后讨论了再现像光场一次位相畸变校正的方法.结果表明,等曲率物参光像面数字全息具有最大的信息容量;该系统的成像分辨率取决于显微物镜的数值孔径和CCD的像元大小,与CCD的光敏面尺寸无关;物体各点中通过显微物镜的所有频率成分均能被系统完全记录与再现,样品被照亮区域的大小对记录条件和再现像质没有影响;等曲率物参光像面数字全息系统是一种优化的全息记录与再现系统,利用该系统可以实现高质量成像.实验结果验证了理论分析的正确性.

High-quality digital image-plane micro-holographic system with the same wavefront curvature of reference and object wave

The digital image-plane holographic microscopy which has the same curvature radius in the object light path and the reference light path is studied for the first time to our knowledge. The configuration of this setup is analyzed and built. The point spread function of this system is derived. The factors corresponding to the imaging resolution and the imaging characteristics of this system are analyzed. The method to correct the linear phase distortion in the reconstructed optical field is discussed. The results show that this system has the largest information content, and the imaging resolution of the system is dependent on the numerical aperture of the microscope objective (MO) and on the CCD pixel size, but independent of the photosensitive surface size of the CCD. All frequency components of each point in the object wave which has passed through the MO can completely be recorded and reconstructed. And the size of illuminated region of object has no influence on the recording condition and the quality of the reconstructed image. The digital image-plane holographic microscopy with the same curvature radius in the object light path and the reference light path is an optimized holographic recording system, which can achieve high-quality imaging. The experimental results demonstrate the correctness of the theoretical analysis.