基于螺旋相位调制的非相干全息点扩散函数研究

分析了菲涅耳非相干相关全息(Fresnel incoherent correlation holography,FINCH)系统中纯相位空间光调制器(spatial light modulator,SLM)加载螺旋相位掩模时的点扩散函数.以氙灯为照明光源搭建了FINCH系统,电荷耦合器记录的点源全息图与点扩散函数模拟结果一致.采用该系统分别在SLM上加载双透镜掩模和螺旋相位调制双透镜掩模两种情况下对分辨率板和非染色洋葱细胞成像,给出了成像对比结果.结果表明:采用螺旋相位调制的FINCH系统可以在几乎不牺牲分辨率的情况下提高图像的边缘对比度;同样,对相位物体也可以实现图像的边缘提取和识别.该方法在实时监测活细胞的分裂、形变等方面具有重要应用前景.     

Improvement of 1D off-axis computer-generated holograms

Creation of Fresnel off-axis computer-generated holograms and improvement of images reconstructed from the holograms are described. To improve the reconstructed images, we have created the holograms with high construct diffraction patterns by using a histogram method. From reconstruction experiments of the holograms, clear images have been obtained and 3D images have been seen directly with eyes.     

基于液晶空间光调制器的全息显示

在传统的纯相位全息显示系统中, 一般基于快速傅里叶变换(FFT)算法来计算相位全息图, 在FFT的计算中需要遵循Nyquist采样定理, 因此, 重建图像的尺寸往往受限于空间光调制器的固定采样率. 这个限制可以通过卷积算法或者两步菲涅耳衍射算法来解决, 但是需要使用多个FFT的计算, 导致计算量增大. 鉴于此, 提出了一种基于透镜的纯相位全息图计算方法. 在全息图的计算中, 通过透镜的成像原理建立一个采样率可变的虚拟全息面, 通过调节相应的距离参数使得在全息图的计算中可以任意调节原始图像的采样率, 摆脱了传统方法中液晶空间光调制器带宽积对重建图像尺寸的限制, 并且这种算法只需使用一次FFT就能达到变采样率的衍射计算, 大幅提高了全息图的计算速度. 数值模拟及光学实验结果证明了此方法可以在全息显示光学系统中清晰地重建不同尺寸的图像. 同时该系统可以有效地消除由空间光调制器的像素化结构带来的零级衍射.     

Elimination of zero-order diffraction in digital off-axis holography

A simple experimental method of eliminating zero-order diffraction in the reconstructed image of off-axis digital holography is presented. Holographic diffraction grating acting as a beam splitter, an off-axis holography system is formed. The holograms of object with different recording parameters are obtained by adjusting the reflecting mirror in the recording optical system to vary the incidence orientation of the object beam in CCD to introduce a phase shift. The zero-order image can be eliminated by numerically processing the holograms of object with different recording parameters. The theoretical analyses have been done in detail and the experimental results are also given. The zero-order image eliminated, the area of reconstructed image increases remarkably, the image quality can then be significantly improved and the better resolution obtained. The experimental results show that the method presented in this paper is feasible, simple in optical system and easy in operation and data processing.     

Image magnified lensless holographic projection by convergent spherical beam illumination

In liquid crystal spatial light modulator(SLM)-based holographic projection, the image is usually displayed at a distant projection screen through free space diffraction from a computer-generated hologram(CGH). Therefore,it allows for removing of the projection lens for the sake of system simplification and being aberration free, known as the "lensless holographic projection". However, the maximum size of the optical projected image is limited by the diffraction angle of the SLM. In this Letter, we present a method for the implementation of image magnification in a lensless holographic projection system by using convergent spherical wave illumination to the SLM.The complete complex amplitude of the image wavefront is reconstructed in a lensless optical filtering system from a phase-only CGH that is encoded by the off-axis double-phase method. The dimensions of the magnified image can break the limitation by the maximum diffraction angle of the SLM at a given projection distance.Optical experiment results with successful image magnification in the lensless holographic projection system are presented.     

显微数字全息中物光波前重建方法研究和比较

根据全息理论和线性系统理论,采用离轴无透镜傅里叶变换全息记录光路,对利用菲涅耳近似法、基于瑞利—索末菲衍射积分的卷积法以及角谱理论方法数值重建全息图进行了比较研究,并做了计算机模拟验证.结果表明：菲涅耳近似法和角谱方法重建像质比较好,且菲涅耳方法重建速度快；在记录距离极小的情况下,尽管记录距离不满足通常的菲涅耳近似条件,菲涅耳近似公式仍然成立；自由空间脉冲响应的快速傅里叶变换的性质与距离有关,由卷积方法得到的再现像只在某一特定距离下比较理想；对于极小物场、大孔径显微数字全息来说,菲涅耳近似重建方法是较为有效的方法.     

一种消除数字离轴全息零级像的实验方法

提出一种消除数字离轴全息零级像的实验方法.这种方法是将全息衍射光栅作为分光元件,形成数字离轴全息记录系统,通过调节记录光路系统中平面镜,改变物光波到达CCD的入射方向而引入相移,从而得到不同记录参数的全息图.再通过对不同记录参数全息图的数字处理,即可达到零级像消除的目的,并对这种方法进行了理论分析和实验验证.给出了利用这种方法获得的实验结果.实验结果证明了该方案的可行性,并且具有光路系统简单、操作和处理容易等特点.     

Wave front reconstruction of Fresnel off-axis holograms with compensation of aberrations by means of phase-shifting digital holography

Off-axis holograms recorded with a CCD camera are numerically reconstructed in amplitude by calculating through the Fresnel–Kirchhoff integral. A phase-shifting Mach–Zehnder interferometer is used for recording four-quadrature phase-shifted off-axis holograms. The basic principle of this technique and its experimental verification are described. We show that the application of this algorithm allows for the suppression of the zero order of diffraction and of the twin image and that the contrast of the reconstructed images can be further enhanced by digital compensation of the aberrations introduced by the holographic recording system     

Digital spatially incoherent Fresnel holography

We present a new method for recording digital holograms under incoherent illumination. Light is reflected from a 3D object, propagates through a diffractive optical element (DOE), and is recorded by a digital camera. Three holograms are recorded sequentially, each for a different phase factor of the DOE. The three holograms are superposed in the computer, such that the result is a complex-valued Fresnel hologram. When this hologram is reconstructed in the computer, the 3D properties of the object are revealed.     

空间光调制器曲面拼接实现全息三维显示视角拓展

本文对多空间光调制器不同拼接方式拓展全息三维再现像视角的方法进行了分析,基于多片空间光调制器拼接拓展视角的思想,利用平面反射镜、分光镜和两片透射式空间光调制器设计了曲面拼接系统,进行了全息三维再现像的视角拓展实验研究。用该系统对四棱锥物体的层析菲涅尔衍射全息图进行再现,结果表明,总视角由基于单片空间光调制器的1.7°增大到3.2°,即拓展到约1.9倍,分光镜能够消除两片空间光调制器间的间隙,实现无缝拼接。该方法同样适用于更多空间光调制器的曲面拼接中,可以有效地拓展全息再现像的视场角大小。     

利用反射全息实现计算全息三维显示

计算全息和光学全息都可应用于三维显示,但各有自己的优势和缺陷.将计算全息和光学反射全息相结合,可以突破光学全息对记录物体的限制,进行虚拟物体或自然场景的全息图的制作,同时可以实现白光再现.本文首先用三维扫描仪获得实际物体的三维数据,用"点云算法"模拟得到其菲涅耳全息图透射率数据,采用计算全息打印机将其输出于全息记录介质,得到可光学再现的菲涅耳计算全息图H1.然后将H1作为光学全息的记录物体进行反射全息记录,将平面全息转化为体全息,实现了计算全息白光再现.     

菲涅耳非相干数字全息大视场研究

菲涅尔非相干相关全息术(Fresnel incoherent correlation holography, FINCH)通过空间光调制器(spatial light modulator, SLM)将来自物点的光波分解为曲率半径不同的两束自相干光,干涉条纹由CCD记录.由于受限于SLM与CCD的像素数目及像素尺寸, FINCH技术与光学全息术相比记录视场要小得多.本文通过对FINCH系统的记录过程进行理论分析,给出了SLM所能记录的视场角,说明通过调控加载在SLM上的双透镜光轴中心,能够扩大SLM的有效直径从而将SLM的有效记录范围增大2.77倍,有效扩大了系统的记录视场.搭建了非相干光反射式数字全息记录系统并对理论分析进行了实验验证,结果表明:在SLM上依次加载不同光轴中心位置的双透镜掩模进行FINCH记录及再现,将得到的各子图像拼接融合可以得到高分辨率大视场图像,为菲涅尔非相干全息术在高分辨大视场显微成像的进一步应用提供了有力支撑.     

Optical-path-length-shifting color digital holography

We propose an in-line color digital holography that can suppress a 0th-order diffraction image and a conjugate image. This technique reconstructs the image of an object from two holograms that are formed at two different distances from the object for each primary color. We numerically simulated the proposed technique and confirmed that it enabled the reconstruction of higher-quality images of an object than the Fresnel transform alone, which is the simplest reconstruction algorithm in digital holography. The effectiveness of the proposed technique was also quantitatively shown by evaluating the reconstructed images using root-mean-square errors. Thus, it was confirmed that the proposed technique was capable of recording and reconstructing both three-dimensional information and color information of an object.     

Generation of three-dimensional optical structures by dynamic holograms displayed on a twisted nematic liquid crystal display

Reconstruction of computer generated holograms (CGHs) addressed on a spatial light modulator (SLM) is an effective way to dynamically generate designed light field distributions. Based on the classic Gerchberg–Saxton (GS) algorithm, we proposed a technique, which can greatly reduce the computation cost to about 60 % in calculating CGHs for three-dimensional (3D) structures but with little degradation of reconstructed light field compared with the classic GS algorithm. The CGHs calculated by our method were displayed on a twisted nematic liquid crystal display, working as a phase-only-modulation SLM, and 3D structures of optical fields, e.g., 3D array of optical traps and vortices, were reconstructed with high efficiency and high quality. Besides, the possibility for 3D holographic display or projection was also demonstrated with this algorithm by reconstruction several images simultaneously in distinct axial planes.     

彩色全息图的计算机产生和数字再现

提出了一种基于色彩合成以及菲涅尔衍射原理,用计算机产生全息图并用数字方法再现彩色全息图的方法。该方法的第一步是将物体的彩色RGB图像分离为三基色强度图,再利用博奇编码的方法制成修正离轴参考光分色计算机全息图;第二步是分别对分色全息图在频域进行调制以实现用原参考光真实再现原始物光波。通过滤波消除零级衍射及共轭像的影响,获得了所需要的实像并提高了像质。提供的实验是选用一幅RGB图像作为原始物体,给出了用博奇编码法制成的全息图以及最后经色彩合成获得的再现像。结果表明,该方法能使各分色全息图的再现像准确重合,解决了在色彩合成时容易出现的色串扰问题。     

基于位相变更的非相干数字全息自适应成像

菲涅耳非相干数字全息作为一种非扫描的三维成像技术具有其独特的优势,但其成像过程中会受到各种像差的影响,导致成像分辨率、再现像的质量降低.为了解决这一问题,可以结合适当的自适应光学技术对波前像差进行探测和校正.位相变更是一种基于两幅具有已知位相差的强度图像实现波前探测和像差校正的技术.本文发展了基于位相变更的非相干数字全息自适应成像技术,不需要引入引导星,利用全息记录过程中的两幅相移全息图,实现波前像差的探测.本文给出了所发展技术的数值仿真和实验结果,结合位相变更算法求解出系统像差的位相分布,将像差的共轭位相加载到光瞳面上,在全息图记录的同时校正像差,从而提高重建像的质量.     

基于像素结构空间光调制器的全息再现像问题研究

基于纯相位空间光调制器的全息显示系统在重构显示时,再现像的视觉效果受到空间光调制器像素结构引起的多级衍射光和多级再现像的干扰。在分析具有有限填充因子空间光调制器的像素结构对再现像影响的基础上,提出了一种提高全息再现像的视觉效果并且再现像成像位置和大小可调节的方法。先加载闪耀光栅到纯相位全息图,其次通过叠加会聚球面波相位,分离再现像与空间光调制器像素结构引起的多级衍射光的聚焦平面的位置,再利用光阑和高通滤波器的共同作用,消除高级衍射光、多级再现像以及零级光干扰对重构视觉效果的影响,最后引入成像透镜,调节再现像的成像位置与大小。建立了一套基于硅基液晶的全息显示系统用于实验验证。实验结果表明,最终的单一再现像清晰且可以方便地调节成像位置和大小。该方法同样适用于各种基于像素结构空间光调制器的全息光学系统。     

Holographic three-dimensional display synthesized from three-dimensional fourier spectra of real existing objects

A method of synthesizing computer-generated holograms of real existing objects is proposed that is based on a series of projection images of an incoherently illuminated object recorded from different perspectives. In accordance with the principles of computer tomography, the three-dimensional Fourier spectrum of the object is calculated by use of several projection images. A method of calculating a Fresnel hologram from the three-dimensional Fourier spectrum is proposed. Experimental results in the form of a computer simulation and optical reconstruction are presented.     

Methods of Fourier optics in digital holographic microscopy

In this paper, processing methods of Fourier optics implemented in a digital holographic microscopy system are presented. The proposed methodology is based on the possibility of the digital holography in carrying out the whole reconstruction of the recorded wave front and consequently, the determination of the phase and intensity distribution in any arbitrary plane located between the object and the recording plane. In this way, in digital holographic microscopy the field produced by the objective lens can be reconstructed along its propagation, allowing the reconstruction of the back focal plane of the lens, so that the complex amplitudes of the Fraunhofer diffraction, or equivalently the Fourier transform, of the light distribution across the object can be known. The manipulation of Fourier transform plane makes possible the design of digital methods of optical processing and image analysis. The proposed method has a great practical utility and represents a powerful tool in image analysis and data processing. The theoretical aspects of the method are presented, and its validity has been demonstrated using computer generated holograms and images simulations of microscopic objects.     

利用相位模板实现数字全息超分辨成像

为了简化数字全息超分辨记录系统,分别在其物光和参考光部分引入一块相位模板,以获得垂直和倾斜方向照明物体的光束和具有不同载波频率的参考光束.当这些具有不同照射方向的光透过物体后,可以使CCD在位置固定的情况下记录到携带低频和高频信息的物体衍射场,不同载波频率的参考光则保证了高频和低频信息在复合全息图的频谱面上能够相互分离.实验结果证明,通过将记录到的物体高频和低频信息合成,可以获得超出系统衍射极限分辨率的再现像.