β-环糊精与荧光黄的包合作用

制备了以β-环糊精为主体,荧光黄为客体的晶体包合物,用红外光谱、DSC曲线、荧光光谱、共振瑞利散射光谱等方法确定了包合物的形成,对包合物的形成进行了初步讨论,并用Hildebrand-Benesi双倒数法测定了包合物的包合常数.实验结果表明:β-环糊精与荧光黄分子形成包合比为1:1的包合物.     

维生素C包合物的拉曼光谱和红外光谱研究

通过拉曼光谱和红外光谱法分析维生素C包合物,探讨拉曼光谱法作为一种新的验证包合物形成方法的可行性。采用饱和水溶液法制备维生素C包合物,共焦拉曼光谱仪和傅里叶转换红外光谱仪分别测定并获得β-环糊精、维生素C、维生素C与β-环糊精物理混合物及其包合物四种固体粉末的拉曼图谱和红外图谱并将其进行对照分析。拉曼图谱和红外图谱共同证明了维生素C通过氢键作用嵌入β-环糊精的疏水空腔形成包合物,从而增加了它的稳定性。拉曼光谱法具有快速、准确、简便、无损测量等显著优势,可作为验证药物包合物的形成重要手段。     

红外漫散射技术用于芹菜素与环糊精包合物的研究

利用红外漫散射技术研究了溶液法和研磨法所制备的芹菜素与羟丙基-β-环糊精包合物的红外光谱。结果显示:1 610cm_(C=C)~(-1)/1 653cm_(C=O)~(-1)谱峰强度的比值和芹菜素与环糊精的包合程度有关,比值小则包合的好。同时,1 590~1 550和1 300~1 200cm~(-1)区域的谱峰变化也可判断芹菜素的包合程度。     

D-樟脑与β-环糊精包合物的结构表征

采用饱和水溶液搅拌法制备β-环糊精(β-CD)/D-樟脑包合物.1H NMR确定了β-CD与D-樟脑形成包合物的化学计量比为1∶1.X-射线衍射图谱和红外光谱图证明D-樟脑和β-CD分子间的相互作用.1H ROESY NMR分析结果说明包合物的结构型式是D-樟脑的二环[2.2.1]-2-庚酮位于β-CD空腔内,其三甲基部分位于β-CD空腔外.通过量子化学计算得到形成β-CD/D-樟脑包合物最低结合能和结构优化状态,氢健的存在证实了上述分析结果的准确性.     

β-环糊精与佐匹克隆包合作用的研究

采用相溶解度法研究了佐匹克隆在不同pH和不同摩尔浓度的β-争环糊精水溶液中的溶解度及二者的表观稳定常数,随着β-环糊精浓度的增加和pH的提高,佐匹克隆的溶解度增加,表观稳定常数升高;采用热力学方法研究了温度对包合反应的影响,计算了包合过程的熵变AS、焓变△H及自由能变化△G均为负值,说明包合反应是放热反应且能自发进行,焓变是形成超分子复合体的主要驱动力;用研磨法制备了佐匹克隆-环糊精包合物,并用红外光谱和差示扫描量热分析对固体包合物进行了表征.结果表明,佐匹克隆与β-环糊精可形成1:1包合物,β-环糊精对于难溶性药物佐匹克隆是较理想的增溶剂.     

Formation of [3]Pseudorotaxanes from β-cyclodextrin and a Series of Dicarboxylic Acids with Their Corresponding α,ω-alkanedicarboxylate Anions

通过研磨法和共沉淀法成功制得β-环糊精与碳原子数11~15的脂肪族二元酸包合物,并通过傅立叶变换红外光谱(FTIR)、差热分析(DTA)以及X射线衍射(XRD)的方法确证了该包合物为[3]准轮烷结构．上述包合物通过与NaOH作用得到了β-环糊精与脂肪族二元酸双负离子的包合物并通过核磁共振氢谱(1H-NMR)及二维NOSEY谱确认了该包合物同样具有[3]准轮烷结构．     

黄芩苷包合物的拉曼光谱和红外光谱研究

通过FT-IR(傅里叶变换红外光谱)和Raman(共焦激光拉曼光谱)分析黄芩苷包合物,对拉曼光谱法作为一种新的验证包合物形成方法的可行性进行验证.采用饱和水溶液法制备黄芩苷包合物,共焦拉曼光谱仪和傅里叶转换红外光谱仪分别测定并获得p-环糊精、黄芩苷、黄芩苷与β-环糊精物理混合物及黄芩苷包合物四种固体粉末的拉曼图谱和红外...     

氯霉素包合物的拉曼光谱和红外光谱研究

研究应用拉曼光谱法分析验证氯霉素包合物的可行性。采用拉曼光谱仪和傅里叶红外光谱仪分别测得β-环糊精、氯霉素、β-环糊精和氯霉素物理混合物、氯霉素包合物四种物质固体粉末的拉曼图谱和红外图谱, 并对其进行对照分析。 结果表明拉曼光谱法可获得与红外光谱法一致的结果, 结果表明氯霉素的苯环结构和易水解基团二氯乙酰胺基通过氢键作用被包裹在β-环糊精的空穴结构中形成包合物, 增加了氯霉素的稳定性和溶出度, 并且拉曼光谱法具有快速、准确、简便、无损测量等显著优势, 可作为验证药物包合物的形成重要手段。     

诺氟沙星与环糊精的超分子体系

研究了诺氟沙星与β-环糊精(简称β-CD)及其两种衍生物羟丙基-β-环糊精(简称Hp-β-CD)、甲基-β-环糊精(简称Me-β-CD)形成的超分子体系.利用荧光光谱法测定了超分子体系的包结常数和包结比,及其热力学参数.诺氟沙星与3种环糊精均形成1∶1稳定的包合物,诺氟沙星与β-CD、Me-β-CD及Hp-β-CD作用...     

磺丁基醚-β-环糊精与尼美舒利的包合光谱学

研究了磺丁基醚-β-环糊精与尼美舒利在水溶液中的包合作用.采用等摩尔系列法测定磺丁基醚-β-环糊精与尼美舒利的包合摩尔比,并利用紫外-可见、红外光谱、扫描电子显微镜法共同验证了包合物的形成.结果表明:磺丁基醚-β-环糊精与尼美舒利以摩尔比1∶1包合,两者形成了新型超分子包合物.     

β-环糊精衍生物对α-苯丙酸包结作用的研究

用紫外光谱法研究了系列β-环糊精衍生物(羟丙基-β-环糊精、羟乙基-β-环糊精和甲基-β-环糊精)对α-苯丙酸的包结作用,考察了不同β-环糊精衍生物对不同存在状态的α-苯丙酸的包结行为。结果说明,α-苯丙酸与β-环糊精衍生物形成的包合物的包合比为1∶1,三种主体环糊精的包结能力强弱顺序为:羟乙基-β-环糊精羟丙基-β-环糊精甲基-β-环糊精。羟丙基-β-环糊精适合包结分子状态的α-苯丙酸,而不易包结离子状态的α-苯丙酸。     

荧光光谱法研究山奈素与β-环糊精及其衍生物包合作用

采用荧光光谱法研究了β-环糊精(β-CD)、甲基-β-环糊精(M-β-CD)、羟丙基-β-环糊精(HP-β-CD)、磺丁基-β-环糊精(SBE-β-CD)与山奈素的包合特性.在固定山奈素浓度和改变环糊精及其衍生物浓度的情况下,山奈素荧光发射波长的变化以及荧光强度的增强表明了包合物的形成,用荧光双倒数法计算了环糊精及其衍...     

羧基-β-环糊精衍生物及其二茂铁包络物的制备与性质研究

制备了一种羧基-β-环糊精衍生物,并将它包络二茂铁,制得了二茂铁-羧基-β-环糊精包络物,用红外光谱、紫外可见光谱、1HNMR、13CNMR等方法表征了羧基-β-环糊精及二茂铁包络物。由于羧基-β-环糊精衍生物破坏了β-环糊精的分子内氢键,并修饰了羧基,使得其水溶性得到明显的提高。研究了包络物的电化学性质,发现其具有良好的氧化还原可逆性和水溶性,可用作电子传递媒介体,循环伏安法测定表明,其检出限为10-7mol/L,表明这类氧化还原具有很高的灵敏度,可以用作性能优异的电化学探针和电流型生物传感器的电子媒介体。     

RP-HPLC测定不同β-环糊精衍生物超分子包合物中丹皮酚的含量

建立了用反相高效液相色谱法测定羟丙基-β-环糊精（HP-β-CD）、甲基-β-环糊精（M-β-CD）以及取代度为1、4、7的3种磺丙基醚-β-环糊精（SPE1-β-CD、SPE4-β-CD、SPE7-β-CD）与丹皮酚（PAE）所形成的超分子包合物中丹皮酚含量的方法。采用Extend C18色谱柱（4.6mm×150mm,5μm）,流动相为甲醇-水（50∶50）,检测波长274nm,流速1mL/min,柱温为25℃。结果表明丹皮酚在4.98—79.68μg/mL浓度范围内线性关系良好（R^2=0.9999）;平均加标回收率分别为96.78%、95.91%、100.57%、99.71%、100.70%;RSD分别为0.92%、1.76%、0.88%、1.69%、0.96%（n=6）。本法可用于β-环糊精衍生物/丹皮酚超分子包合物中丹皮酚的含量测定。     

Synthesis and characterization of β-cyclodextrin/fraxinellone inclusion complex and its influence on interaction with human serum albumin

Aqueous solubility is one of the key determinants in developing new chemical entities as drugs. In this study, to improve the solubility of fraxinellone, a novel β-cyclodextrin/fraxinellone inclusion complex was prepared and characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and elemental analysis. Moreover, the influence of inclusion of small molecule by cyclodextrins on its binding with transporter in human body is essential for drug development. For the first time, we report the interaction of fraxinellone and its inclusion complex with human serum albumin. Fluorescence quenching of albumin by fraxinellone and its inclusion complex is a static process through complex formation. The results indicated that β-cyclodextrin did not affect the binding force and site of fraxinellone to the protein. Furthermore, circular dichroism showed that both fraxinellone and its inclusion complex induced a significant change in the secondary structure of human serum albumin.     

Encapsulation of Labetalol, Pseudoephedrine in β-cyclodextrin Cavity: Spectral and Molecular Modeling Studies

The absorption and fluorescence spectra of labetalol and pseudoephedrine have been studied in different polarities of solvents and β-cyclodextrin (β-CD). The inclusion complexation with β-CD is investigated by UV-visible, steady state and time resolved fluorescence spectra and PM3 method. In protic solvents, the normal emission originates from a locally excited state and the longer wavelength emission is due to intramolecular charge transfer (TICT). Labetalol forms a 1:2 complex and pseudoephedrine forms 1:1 complex with β-CD. Nanosecond time-resolved studies indicated that both molecules show triexponential decay. Thermodynamic parameters (ΔG, ΔH, ΔS) and HOMO, LUMO orbital investigations confirm the stability of the inclusion complex. The geometry of the most stable complex shows that the aromatic ring is deeply self included inside the β-CD cavity and intermolecular hydrogen bonds were established between host and guest molecules. This suggests that hydrophobic effect and hydrogen bond play an important role in the inclusion process.     

Supramolecular Study on the Interaction Between Ofloxacin and Methyl β-Cyclodextrin by Fluorescence Spectroscopy and its Analytical Application

The supramolecular interaction of ofloxacin (Oflo) and methyl β-cyclodextrin (Mβ-CD) has been examined by UV–vis, IR and fluorescence spectroscopy. The formation of inclusion complex has been confirmed based on the changes of the spectral properties. The results showed that Mβ-CD reacted with Oflo to form an inclusion complex. The Oflo and Mβ-CD complex formed a host-guest complex in 1:1 stoichiometry and inclusion constant (K?=?7.8?×?10^?3 L mol^?1) was ascertained by the typical double reciprocal plots. Furthermore, the thermodynamic parameters (?H°, ?S° and ?G°) associated with the inclusion process were also determined. In addition, solid inclusion complex was synthesized. Based on the significant enhancement of the fluorescence intensity of Oflo produced through complex formation, a simple, accurate, rapid and highly sensitive spectrofluorometric method for the determination of Oflo in pharmaceutical formulation was developed. The measurement of relative fluorescence intensity was carried out at 497 nm with excitation at 296 nm. The factors affecting the inclusion complex formation were studied and optimized. Under the optimum reaction conditions, linear relationships with good correlation coefficients (0.9995) were in the concentration range of 50–350 ng/mL for spectrofluorimetry. The limit of detection (LOD) was 11.5 ng/mL. The proposed method was successfully applied to the analysis of Oflo in pharmaceutical preparation.     

薰衣草精油微胶囊释放过程的红外光谱研究

为了解薰衣草精油β-环糊精包合物的构成形式和精油随温度变化的缓释过程,利用傅里叶变换红外变换光谱(FTIR)技术,对薰衣草精油(LO)、β-环糊精(β-CD)及薰衣草精油微胶囊(LOM)进行红外光谱对比分析,同时对不同温度下精油缓释过程中微胶囊红外光谱变化进行了分析,并结合主成分分析,进一步探究β-CD包埋LO后物理化学稳定性及LO释放过程。结果表明,LO包埋后其特征峰有红移现象、峰形变宽,这主要受形成分子间氢键、p-π共轭现象和β-CD空间结构影响;另外,设定温度变化范围25～95 ℃,温度间隔10 ℃,测定LOM的红外光谱,以考证β-CD包埋LO的物理化学稳定性及释放情况,分析实验结果表明,LOM中水合物分子较易失去,LOM中的精油组分物理化学性质稳定,在95 ℃时逸出量小于6.5%,释放缓慢;对变温红外光谱数据进行主成分分析(PCA),前二个主成分的累积方差为99.3%,通过主成分载荷分析,PC1成分可认为是β-CD特征变量,PC2成分为LO特征变量,主成分结果表明,LOM包埋精油中酯类物质的释放速度要快于醇类物质。采用红外分析方法简便快捷,全面了解包埋精油的物理化学稳定性和释放过程,为薰衣草精油微胶囊释放过程的研究提供新的理论支持。