共查询到20条相似文献,搜索用时 31 毫秒
1.
Simple and rapid analytical procedures for the ETAAS determination of Cd and Pb in plant (poplar, clover, plantain) leaves
are described. Optimal conditions are presented for the wet digestion of plant matrices with a mixture of nitric acid and
hydrogen peroxide along with optimal temperature programmes for subsequent modifier-free electrothermal determination of Cd
and Pb. Slurry preparation procedures using tetramethylammonium hydroxide or a mixture of 1 mol/L HNO3 + 1.5 mol/L H2O2 are proposed as another approach for rapid and accurate routine analysis of Cd and Pb in plant leaves. It has been found
that single standard addition can be used for quantitative determination in the case of wet digestion ETAAS and standard addition
to each analysed matrix is recommended in the case of slurry ETAAS. For all plant leaves investigated good agreement was achieved
between the concentrations determined by wet digestion ETAAS and slurry ETAAS. The wet digestion method provides relative
standard deviations for Cd ranging from 3% to 17% and for Pb from 2% to 16%. For the slurry method the RSD values are in the
range of 4–31% for Cd and 4–30% for Pb, depending for both cases on the magnitude of the measured concentrations. Validity
and versatility of the methods are verified by the analyses of standard reference materials.
Received: 17 March 1997 / Revised: 2 May 1997 / Accepted: 8 May 1997 相似文献
2.
P. Bermejo-Barrera Antonio Moreda-Piñeiro Jorge Moreda-Piñeiro Adela Bermejo-Barrera 《Analytical and bioanalytical chemistry》1997,358(7-8):844-847
Rapid methods were developed for the direct determination of Ag, Al, Cd and Mn in cocaine and heroin by ETAAS using programmes
omitting the charring step . Sample pretreatment was simple: dissolution in ultrapure water or in 35.0% (v/v) HNO3 for heroin or cocaine, respectively. Optimum drying temperatures were 250 °C for Ag, Al and Mn, and 300 °C for Cd. The run
cycles were 35 and 37 s, for Ag and Al respectively, and 36 s for Cd and Mn. The best results were obtained with Pd, Mg(NO3)2 and (NH4)2HPO4, as chemical modifiers. The limits of detection were 8.6, 55.9, 2.2 and 12.4 μg kg-1 for Ag, Al, Cd and Mn, respectively.
Received: 14 November 1996/Revised: 14 January 1997/Accepted: 18 January 1997 相似文献
3.
Carlos E. C. Magalhães Éder C. Lima Francisco J. Krug Marco A. Z. Arruda 《Mikrochimica acta》1999,132(1):95-100
A method for direct analysis of tea and coffee samples by using electrothermal atomic absorption spectrometry is described.
Coffee and tea from different sources were analyzed without digestion step. For slurry analyses the samples were ground, sieved
at 105 μm and then suspended in 0.2% v/v HNO3 and 10% v/v Triton X-100 medium. For liquid phase aluminium determination the samples were prepared in the same way and only
the liquid is introduced directly into the graphite furnace. Calibration was performed by aqueous standards for both cases
and the determinations were carried out in the linear range between 50 and 250 μg L−1. The characteristic mass of aluminium and the detection limit were 45 pg and 2 μg L−1, respectively. Using a typical 0.1% m/v coffee slurry sample, the relative standard deviation of measurements (n=15) for
repeatability was about 8.2%.
Received December 27, 1998. Revision March 18, 1999. 相似文献
4.
Lina Kantiani Marinella Farré Josep Manuel Grases i Freixiedas Damià Barceló 《Analytical and bioanalytical chemistry》2010,398(3):1195-1205
A fully automated method has been developed for analysis of eighteen antibacterial compounds, including penicillins, cephalosporins
and sulfonamides, in animal feed with limits of quantification in the range 0.25–5.79 μg kg−1. The method is based on pressurized liquid extraction of 3 g homogenized feed with water and online clean-up of 500 μL of
the extract with C18HD cartridges. The purified sample was directly analysed by liquid chromatography–electrospray tandem mass spectrometry (SPE–LC–ESI-MS–MS).
Chromatographic separation was achieved within 10 min by use of a C12 Phenomenex Hydro-RP reversed-phase analytical column and a mobile phase gradient (water + 0.1% formic acid–methanol + 0.1%
formic acid). The method was validated, revealing capability for detection of concentrations as low as 0.09 μg kg−1, decision limits (CCα) and detection capabilities (CCβ) in the range 10–174 μg kg−1 and 22–182 μg kg−1, respectively, and inter-day precision ranging from 0.7 to 8.3%. Recovery, with internal standard correction, was in the
range 93–134% for all analytes. The method was then applied to analysis of fifteen feed samples, nine of which contained at
least one antimicrobial at concentrations between 0.006 and 1.526 mg kg−1. The performance data and results from the method were compared with those from a previous method developed by our group,
using offline SPE, by analyzing the same set of samples by both methods. The online SPE approach resulted in slightly improved
sensitivity, with LODs of 0.09–2.12 μg kg−1 compared with 0.12–3.94 μg kg−1 by the offline approach. In general, better recovery was achieved by use of online purification (for 72% of the analytes)
and the correlation between the two methods was good. The main advantages of the new online method are rapid and automated
sample pre-treatment, and reduction of sample manipulation, enabling high-throughput analysis and highly accurate results.
Because of all these characteristics, the proposed method is applicable and could be deemed necessary within the field of
food control and safety. 相似文献
5.
A flow injection system was developed for on-line sorbent extraction preconcentration and flame atomic absorption spectrometric
determination of cadmium in natural water samples. The non-charged cadmium complex with diethyl-dithiophosphate (DDPA) was
formed on-line in 0.1 mol L−1 HNO3 and retained on the hydrophobic poly-chlorotrifluoroethylene (PCTFE) sorbent material. The adsorbed complex was eluted with
isobutyl methylketone (IBMK) and injected directly into the nebulizer via a flow compensation unit. All major chemical and
flow parameters affecting the complex formation adsorption and elution as well as interference were studied and optimized.
By processing 2.4 mL of sample, the enhancement factor was 39 and the sampling frequency was 50 h−1. For 30 s preconcentration time the detection limit was 0.3 μg L−1 and the relative standard deviation at 5.0 μg L−1 Cd concentration level was 2.9%. The calibration curve was linear in the range 0.8–40.0 μg L−1. The accuracy of the method was estimated by analyzing a certified reference material NIST-CRM 1643d (Trace elements in water).
Good recoveries were obtained for spiked natural-water and waste-water samples.
Correspondence: Aristidis N. Anthemidis, Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University,
GR-Thessaloniki 54124, Greece 相似文献
6.
Liu X Xu J Li Y Dong F Li J Song W Zheng Y 《Analytical and bioanalytical chemistry》2011,399(7):2539-2547
A sensitive and effective method for simultaneous determination of triazolopyrimidine sulfonamide herbicide residues in soil,
water, and wheat was developed using ultra-performance liquid chromatography coupled with tandem mass spectrometry. The four
herbicides (pyroxsulam, flumetsulam, metosulam, and diclosulam) were cleaned up with an off-line C18 SPE cartridge and detected
by tandem mass spectrometry using an electrospray ionization source in positive mode (ESI+). The determination of the target
compounds was achieved in <2.0 min. The limits of detection were below 1 μg kg−1, while the limits of quantification did not exceed 3 μg kg−1 in different matrices. Quantitation was determined from calibration curves of standards containing 0.05–100 μg L−1 with r
2 > 0.997. Recovery studies were conducted at three spiked levels (0.2, 1, and 5 μg kg−1 for water; 5, 10, and 100 μg kg−1 for soil and wheat). The overall average recoveries for this method in water, soil, wheat plants, and seeds at three levels
ranged from 75.4% to 106.0%, with relative standard deviations in the range of 2.1–12.5% (n = 5) for all analytes. 相似文献
7.
M. López-Sánchez M. J. Ayora-Cañada A. Molina-Díaz M. Siam W. Huber G. Quintás S. Armenta B. Lendl 《Analytical and bioanalytical chemistry》2009,394(8):2137-2144
A mid-infrared enzymatic assay for label-free monitoring of the enzymatic reaction of fructose-1,6-bisphosphatase with fructose
1,6-bisphosphate has been proposed. The whole procedure was done in an automated way operating in the stopped flow mode by
incorporating a temperature-controlled flow cell in a sequential injection manifold. Fourier transform infrared difference
spectra were evaluated for kinetic parameters, like the Michaelis–Menten constant (K
M) of the enzyme and V
max of the reaction. The obtained K
M of the reaction was 14 ± 3 g L−1 (41 μM). Furthermore, inhibition by adenosine 5′-monophosphate (AMP) was evaluated, and the K
MApp value was determined to be 12 ± 2 g L−1 (35 μM) for 7.5 and 15 μM AMP, respectively, with V
max decreasing from 0.1 ± 0.03 to 0.05 ± 0.01 g L−1 min−1. Therefore, AMP exerted a non-competitive inhibition. 相似文献
8.
Elif Ant Bursali Melek Merdivan Muruvvet Yurdakoc 《Journal of Radioanalytical and Nuclear Chemistry》2010,283(2):471-476
Olive cake as low-cost abundantly available sorbent has been characterized by N2 at 77 K adsorption, porosity analysis, elemental analysis and IR spectra and has been used for preconcentrating of uranium(VI)
and thorium(IV) ions prior to their determination spectrophotometrically. The optimum pH values for quantitative sorption
of U(VI) and Th(IV) are 4–7 and 3–7, respectively. The enrichment factor for the preconcentration of U(VI) and Th(IV) were
found to be 125 and 75 in the given order. The sorption capacity of olive cake is in the range of 2,260–15,000 μg g−1 for Th(IV) and in the range of 1,090–17,000 μg g−1 for U(VI) at pH 3–7. The sorbent exhibits good reusability and the uptake and stripping of the studied ions were fairly rapid.
The elution of U(VI) and Th(IV) was performed with 0.3–1 M HCl/1–2 M HNO3 and 0.3–0.8 M HCl/1 M HNO3, respectively. The precision of the method was 1.8 RSD% for U(VI) and 2.5 RSD% for Th(IV) in a concentration of 1.00 μg mL−1 for 10 replicate analysis. The influence of some electrolytes and cations as interferents was discussed. Separation of U(VI)
and Th(IV) from other metal ions in synthetic solution was achieved. 相似文献
9.
This work presents a comparison of convective interaction media (CIM) and controlled pore glass (CPG) as solid supports for
immunoglobulin antibodies used in bioanalytical detection of allergens in foodstuffs. A flow-injection manifold with highly
sensitive thermal lens spectrometric detection was used for this purpose. Using beta-lactoglobulin, a milk allergen, as a
model analyte, CIM disc supports had a higher linear range (0.2–3.5 μg L−1), better reproducibility (intra-day RSD = 1%, inter-day RSD = 10%), lower consumption of reagents, and better immunocolumn
stability (1 month, over 240 injections of substrate), while providing comparable LODs (0.1 μg L−1). Application of CIM discs as solid supports in immunocolumns for allergen detection enables fast and sensitive screening
of allergens in foodstuffs with sample throughput of up to eight samples per hour. 相似文献
10.
Samuel Melaku Ilse Gelaude Frank Vanhaecke Luc Moens Richard Dams 《Mikrochimica acta》2003,142(1-2):7-12
Microwave digestion reduction-aeration and pyrolysis combined with cold vapour atomic absorption and cold vapour atomic fluorescence
are compared for the determination of total mercury in several biological and environmental matrices. The biological samples
were digested in a mixture of HNO3/H2O2, the environmental samples in a mixture of HNO3/HClO4. After reduction with SnCl2, the mercury was collected by two-stage gold amalgamation. After microwave digestion reduction-aeration, detection limits
of 1.4 ng g−1 and 0.6 ng g−1 were obtained for cold vapour atomic absorption spectrometry (CVAAS) and cold vapour atomic fluorescence spectrometry (CVAFS),
respectively, for 250 mg of environmental samples. For biological samples (500 mg) the detection limits were 0.7 ng g−1 (CVAAS) and 0.4 ng g−1 (CVAFS). After pyrolysis, detection limits of 3.5 ng g−1 and 1.6 ng g−1 for CVAAS and CVAFS, respectively, were obtained for a 10 mg sample. Pyrolysis can only be applied when the organic content
of the sample is not too high. Accurate results were obtained for 8 certified reference materials of both environmental and
biological origin. In addition, a real sludge sample was analysed.
Author for correspondence. E-mail: richard.dams@rug.ac.be
Received September 18, 2002; accepted December 3, 2002
Published online May 5, 2003 相似文献
11.
Irina Karadjova Julijana Cvetković Trajče Stafilov Sonja Arpadjan 《Central European Journal of Chemistry》2007,5(3):739-747
The parameters of analytical procedures developed for direct ETAAS determination of Pb in wine are discussed. Atomic absorption
spectrometers based on transversal and longitudinal Zeeman effect, wall and integrated platform atomization with two main
approaches: (i) measurements in the presence of modifier and (ii) measurements without using any modifier are compared. The
optimal temperature programs are defined according to the pre-treatment and atomization curves constructed in the presence
of different types of wines. For all investigated instrumental systems, 1:1 dilution of wine sample with 0.2 mol L−1 HNO3 is recommended. Matrix interferences observed, call for standard addition calibration method for Pb quantification in wines.
The detection limit (3σ) achieved for wine diluted in the ratio of 1:1 varied from 0.8 to 1.9 μg L−1 depending on the instrument used. The relative standard deviation for the concentration range of 10 to 80 μg L−1 Pb in wine is typically between 4–8%. The accuracy of the analytical procedures recommended was confirmed by comparing the
results obtained with those found for wine samples previously digested with HNO3-H2O2 mixture, by added/found method and by parallel analysis using different instruments. A total of 66 wine samples from different
regions of Macedonia were analyzed.
相似文献
12.
R. K. Singhal P. K. Sharma M. K. T. Bassan H. Basu A. V. R. Reddy 《Journal of Radioanalytical and Nuclear Chemistry》2011,288(1):149-156
During this work selective separation of uranium from rock phosphate and columbite mineral was done before its quantitative
estimation by using Inductively Coupled Plasma Optical Emission Spectrometery (ICP-OES). Uranium from the rock phosphate and
columubite was extracted by sodium peroxide fusion followed by leaching in 2 M HNO3. To avoid spectral interference in the estimation of uranium by ICP-OES, the selective separation of uranium from the leachate
was carried out by using two different extractants, 30% Tributyl Phophates (TBP) in CCl4 and a equi-volume mixture of Di(2-ethylhexyl) phosphoric acid (D2EHPA) & TBP in petrofin. Uranium was stripped from the organic
phase by using 1 M ammonium carbonate solution. Determination of uranium by ICP-OES was done after dissolving the residue
left after evaporation of ammonium carbonate solution in 4% HNO3. The concentration of the uranium observed in the rock phosphates samples was 40–200 μg g−1 whereas in columbite samples the concentration range was 100–600 μg g−1. Uranium concentration evaluated by ICP-OES was complimented by gamma & alpha spectrometry. Concentration of uranium evaluated
by gamma spectrometry in case of rock phosphate and coulmbite was in close agreement with the uranium content obtained by
ICP-OES. Uranium determination by alpha spectrometry showed only minor deviation (1–2%) from the results obtained by ICP-OES
in case of rock phosphates whereas in case of coulmbites results are off by 20–30%. 相似文献
13.
A new adsorbent is proposed for the solid-phase extraction of phenol and 1-naphthol from polluted water. The adsorbent (TX-SiO2) is an organosilica composite made from a bifunctional immobilized layer comprising a major fraction (91%) of hydrophilic
diol groups and minor fraction (9%) of the amphiphilic long-chain nonionic surfactant Triton X-100 (polyoxyethylated isooctylphenol)
(TX). Under static conditions phenol was quantitatively extracted onto TX-SiO2 in the form of a 4-nitrophenylazophenolate ion associate with cetyltrimethylammonium bromide. The capacity of TX-SiO2 for phenol is 2.4 mg g−1 with distribution coefficients up to 3.4 × 104 mL g−1; corresponding data for 1-naphthol are 1.5 mg g−1 and 3 × 103 mL g−1. The distribution coefficient does not change significantly for solution volumes of 0.025–0.5 L and adsorbent mass less than
0.03 g; 1–90 μg analyte can be easily eluted by 1–3 mL acetonitrile with an overall recovery of 98.2% and 78.3% for phenol
and 1-naphthol, respectively. Linear correlation between acetonitrile solution absorbance (A
540) and phenol concentration (C) in water was found according to the equation A
540 = (6 ± 1) × 10−2 + (0.9 ± 0.1)C (μmol L−1) with a detection range from 1 × 10−8 mol L−1 (0.9 μL g−1) to 2 × 10−7 mol L−1 (19 μL g−1), a limit of quantification of 1 μL g−1 (preconcentration factor 125), correlation coefficient of 0.936, and relative standard deviation of 2.5%. A solid-phase colorimetric
method was developed for quantitative determination of 1-naphthol on adsorbent phase using scanner technology and RGB numerical
analysis. The detection limit of 1-naphthol with this method is 6 μL g−1 while the quantification limit is 20 μL g−1. A test system was developed for naked eye monitoring of 1-naphthol impurities in water. The proposed test kit allows one
to observe changes in the adsorbent color when 1-naphthol concentration in water is 0.08–3.2 mL g−1. 相似文献
14.
dos Santos EJ Herrmann AB Frescura VL Welz B Curtius AJ 《Analytical and bioanalytical chemistry》2007,388(4):863-868
Among the “traditional” hydride-forming elements, lead is probably the most difficult, and its determination in this form
has rarely been reported in the literature. In this paper a simple and rapid method, axial-view inductively-coupled plasma
optical-emission spectrometry using on-line hydride generation (HG–ICP–OES) from samples prepared as slurry, is proposed for
determination of lead in environmental samples. The samples (20–50 mg, particle size ≤120 μm) were treated with 1 mL aqua
regia in a 40-kHz ultrasonic bath for 60 min. The slurry was diluted to a final volume of 50 mL with a 10% m/v solution of (NH4)2S2O8. The concentrations of NaBH4, tartaric acid, and (NH4)2S2O8, used for on-line plumbane generation were optimized by means of a complete factorial analysis applied to an aqueous standard
solution and to the slurry of a sediment certified reference material (CRM). External calibration against aqueous standards
in the concentration range 10–100 μg L−1 was used for analysis of six CRM—three marine sediments, one river sediment, and two sewage sludges. Analysis of the filtered
slurry showed that Pb was only partially extracted into the liquid phase. Several major concomitants tested did not affect
the Pb signal. The detection limit (3s, n = 10) for 20 mg sample in a final volume of 50 mL was 5.0 μg g−1. Tin was the only other hydride-forming analyte that could be determined satisfactorily with Pb; for tin the detection limit
was 1.0 μg g−1. The values obtained for Pb and Sn were not significantly different from the certified concentrations, according to the t-test at the 95% confidence level. Nine river sediments collected locally were also analyzed and the concentrations were in
agreement with results obtained after total digestion. 相似文献
15.
Sorption of copper on filter-paper with chemically attached hexamethylenediamino-groups (HMDA-filter) allows to obtain the
sorbent (Cu/HMDA-filter) stable in respect to desorption of copper. A nitrogen-containing herbicide imazapyr (imaz) is retained on Cu/HMDA-filters at pH 5.5–7.0 forming a relatively stable complex. Imazapyr is determined directly on the
sorbent by its activating effect in the oxidation of hydroquinone with H2O2 catalyzed by Cu(II) with the formation of a product absorbing at 490 nm. The copper ions serve both to preconcentrate imazapyr
and to catalyze the indicator reaction. The use of 1-μL sample aliquots pipetted onto the Cu/HMDA-filters allows to determine
1 × 10−3–0.03 μmol of imazapyr, whereas preconcentration of the analyte by pumping of its solution through the same sorbent expands
the linear range to 1 × 10−4–1 × 10−1 μmol of imazapyr. When the indicator reaction is carried out in solution, the range of activating action of imazapyr is narrower
(0.06–0.1 μmol a for a solution volume of 10 mL). The determination is selective: 5–100-fold amounts of amines, aminoacids,
carboxylic acid derivatives and other model compounds do not interfere. Soil extracts and carrot juice samples spiked with
imazapyr have been analyzed.
Received January 10, 2000. Revision July 28, 2000. 相似文献
16.
A new method of direct single-drop microextraction combined with electrothermal atomic absorption spectrometry (ETAAS) is
presented for the determination of trace Cd and Pb with dithizone (H2DZ) as chelating reagent. Factors influencing the microextraction efficiency and determination, such as pH, microdrop volume,
stirring rate, extraction time were evaluated. Under the optimized experimental conditions, the detection limits of the method
are 2 and 90 pg mL−1 for Cd and Pb, and the relative standards deviations for 0.5 ng mL−1 Cd and 10 ng mL−1 Pb are 11 and 12.8%. After 10 min of extraction, the enrichment factors for Cd and Pb are 118 and 90, respectively. The results
for the determination of Cd and Pb in tap water, spring water, river water, pond water, lake water and spiked water samples
demonstrate the accuracy, recovery and applicability of the method. An environmental water certified reference material (GSBZ
50009-88) was analyzed, and the determined values are in a good agreement with the certified values.
Correspondence: Bin Hu, Department of Chemistry, Wuhan University, Wuhan 430072, P.R. China 相似文献
17.
Jadwiga Opydo 《Mikrochimica acta》2001,137(3-4):157-162
Necessary conditions were established for simultaneous nickel and cobalt determination in environmental samples, such as
oak wood and soil, based on cathodic adsorptive stripping voltammetry. Ni(II) and Co(II), complexed with dimethylglyoxime,
were determined using a hanging mercury drop electrode. Optimum conditions were found to be: accumulation time 90 s, accumulation
potential −0.80 V vs. SCE, supporting electrolyte 0.2 mol dm−3 ammonia-ammonium chloride buffer (pH = 9.4) + 0.05 mol dm−3 NaNO2 and dimethylglyoxime 2 × 10−4 mol dm−3. A linear current-concentration relationship was observed up to 7.51×10 −7 mol dm−3 for Ni(II) and 7.0 × 10−7 mol dm−3 for Co(II). Excess amounts of zinc(II) interfering with cobalt peaks were masked by complexation with EDTA. Wood and soils
were mineralized by applying a microwave digestion system, using the mixtures H2O2 + HNO3 or HNO3 + HF, respectively. The developed procedure was tested by analysing international reference materials (BCR 62 Olive Leaves
and GBW 08302 Tibet Soil). The developed procedure was used to determine pollution of oak stand with nickel and cobalt in
different regions of Poland.
Received August 10, 2000. Revision May 22, 2001. 相似文献
18.
Ultrasonic slurry sampling electrothermal vaporization dynamic reaction cell inductively coupled plasma mass spectrometry
(USS–ETV–DRC–ICP–MS) for the determination of Cr, Cd and Pb in several plastic samples, using NH4NO3 as the modifier, is described. The influences of the instrumental operating conditions and the slurry preparation technique
on the ion signals are investigated. A reduction in the intensity of the background at signals corresponding to chromium masses
(arising from matrix elements) was achieved by using NH3 as the reaction cell gas in the DRC. The method was applied to determine Cr, Cd and Pb in two polystyrene (PS) samples and
a polyvinyl chloride (PVC) sample using two different calibration methods, namely standard addition and isotope dilution.
The results were in good agreement with those for digested samples analyzed by ultrasonic nebulization DRC–ICP–MS. The precision
between sample replicates was better than 17% with the USS–ETV–DRC–ICP–MS method. The method detection limits, estimated from
standard addition curves, were about 6–9, 1–2 and 8–11 ng g−1 for Cr, Cd and Pb, respectively, in the original plastic samples. 相似文献
19.
A fast and sensitive liquid chromatography–mass spectrometry method was developed for the determination of ursolic acid (UA)
in rat plasma and tissues. Glycyrrhetinic acid was used as the internal standard (IS). Chromatographic separation was performed
on a 3.5 μm Zorbax SB-C18 column (30 mm × 2.1 mm) with a mobile phase consisting of methanol and aqueous 10 mM ammonium acetate
using gradient elution. Quantification was performed by selected ion monitoring with (m/z)− 455 for UA and (m/z)− 469 for the IS. The method was validated in the concentration range of 2.5 − 1470 ng mL−1 for plasma samples and 20 − 11760 ng g−1 for tissue homogenates. The intra- and inter-day assay of precision in plasma and tissues ranged from 1.6% to 7.1% and 3.7%
to 9.0%, respectively, and the intra- and inter-day assay accuracy was 84.2 − 106.9% and 82.1 − 108.1%, respectively. Recoveries
in plasma and tissues ranged from 83.2% to 106.2%. The limits of detections were 0.5 ng mL−1 or 4.0 ng g−1. The recoveries for all samples were >90%, except for liver, which indicated that ursolic acid may metabolize in liver. The
main pharmacokinetic parameters obtained were T
max = 0.42 ± 0.11 h, C
max = 1.10 ± 0.31 μg mL−1, AUC = 1.45 ± 0.21 μg h mL−1 and K
a = 5.64 ± 1.89 h−1. The concentrations of UA in rat lung, spleen, liver, heart, and cerebellum were studied for the first time. This method
is validated and could be applicable to the investigation of the pharmacokinetics and tissue distribution of UA in rats. 相似文献
20.
A simple, rapid, selective, sensitive and economical method has been developed for the simultaneous determination of trace
amounts of palladium and nickel in aqueous methanolic medium using 2-(2-thiazolylazo)-5-dimethylam inobenzoic acid as an analytical
reagent by first derivative spectrophotometr y. Palladium is determined by measuring base to peak distance at λ=695.0 nm while
nickel is estimated by zero crossing method in the mixture. The linearity is maintained between 0.12–1.75 μg mL−1 for palladium and 0.07–1.60 μg mL−1 for nickel in the pH range 2.8–7.2 and 3.4–8.8 respectively. Seven replicate determinations of 1.0 μ g mL−1 of palladium and 0.8 μg mL−1 of nickel in a mixture give a mean signal height of 0.391 for Pd and 0.541 for Ni with relative standard deviations of 0.9%
and 1.2%, respectively. The sensitivity of the proposed method is 0.391 (dA/dλ)/(μg mL−1) for palladium and 0.685 (dA/dλ)/(μg mL−1) for nickel. Various parameters have been optimised for the simultaneous determination of palladium and nickel in various
complex samples.
Received March 30, 1999. Revision November 25, 1999. 相似文献