共查询到19条相似文献,搜索用时 656 毫秒
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高分子对酶,抗体DNA的修饰,固定化及其生物医学应用 总被引:2,自引:0,他引:2
马建标 《高等学校化学学报》1997,18(7):1227-1235
为发展适于生物医用的生物功能高分子材料,本实验室近年来研究了可溶性高分子对L-天冬酰胺酶的修饰,纳米磁性高分子微粒对酶或抗体的固定化,亚微米高分子微球固定化碱性磷酸酶及其在DNA检测中的应用,高分子微球固定化酶的合成与性能,酶在导电高分子膜上的固定化及生物传感器制备等,本文对此进行简要总结。 相似文献
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选用自制的磁性纳米四氧化三铁为酶固定化的载体,通过交联法来固定葡萄糖氧化酶。通过对酶固定化过程中一系列因素的研究,确定出酶固定化的最佳工艺为:交联剂戊二醛与浓度为0.7 mg/mL酶液的体积比为1:4,固定化时间为0.5 h,固定化温度为室温(≤20 ℃),固定化所用缓冲液pH=7。采用比色法测定其酶活力。结果表明葡萄糖氧化酶经过固定化后,其酶活力较游离酶显著增加。固定化酶的热稳定性、酸碱稳定性、存储稳定性都有很大的提高。 相似文献
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再生丝素固定葡萄糖氧化酶及其传感器应用 总被引:7,自引:0,他引:7
再生丝素固定葡萄糖氧化酶及其传感器应用钱江红,刘永成,刘海鹰,于同隐,邓家祺(复旦大学化学系高分子科学系,上海,200433)关键词再生丝素,葡萄糖氧化酶,传感器,酶电极酶电报的各项性能在很大程度上取决于酶的固定比方法,葡萄糖氧化酶的固定化方法很多1... 相似文献
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本文利用壳聚糖的絮凝性质,将葡萄糖氧化酶包埋在壳聚糖与多聚磷酸盐的絮絮沉淀中,操作简单,固定化效率高。研究了适合了酶电极使用的最佳固定化条件。 相似文献
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高分子对酶、抗体、DNA的修饰、固定化及其生物医学应用 总被引:5,自引:0,他引:5
马建标 《高等学校化学学报》1997,18(7):1227-1235
为发展适于生物医用的生物功能高分子材料,本实验室近年来研究了可溶性高分子对L-天冬酰胺酶的修饰、纳米磁性高分子微粒对酶或抗体的固定化、亚微米高分子微球固定化碱性磷酸酶及其在DNA检测中的应用、高分子微球固定化酶的合成与性能、酶在导电高分子膜上的固定化及生物传感器制备等. 本文对此进行简要总结. 相似文献
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Gluconic acid was obtained in the permeate side of the bioreactor with glucose oxidase (GOD) immobilized onto anion-exchange membrane (AEM) of low-density polyethylene grafted with 4-vinylpiridine. The electric resistance of the anion-exchange membranes was increased after the enzyme immobilization on the membrane. The gluconic acid productions were relatively low with the GOD immobilized by any method on the AEM. To increase the enzyme reaction efficiency, GOD was immobilized on membrane of AN copolymer (PAN) adjacent to an anion-exchange membrane in bioreactor. Uses of anion-exchange membrane led to selective removal of the gluconic acid from the glucose solution and reduce the gluconic acid inhibition. The amount of gluconic acid obtained in the permeate side of the bioreactor with the GOD immobilized on the PAN membrane adjacent to the AEM under electrodialysis was about 30 times higher than that obtained with enzyme directly bound to the AEM. The optimal substrate concentration in the feed side was found to be about 1 g/l. Further experiments were carried out with the co-immobilized GOD plus Catalase (CAT) on the PAN membrane adjacent to the AEM to improve the efficiency of the immobilize system. The yield of this process was at least 95%. The storage stability of the co-immobilized GOD and CAT was studied (lost 20% of initial activity for 90 d). The results obtained clearly showed the higher potential of the dual membrane bioreactor with GOD plus CAT bound to ultrafiltration polymer membrane adjacent to the AEM. Storage stability of GOD activity in GOD plus CAT immobilized on PAN//AEM membranes and on AEM. 相似文献
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Yanagisawa K Murakami TN Tokuoka Y Ochiai A Takahashi M Kawashima N 《Colloids and surfaces. B, Biointerfaces》2006,48(1):67-71
Adsorption condition and enzymatic activity of glucose oxidase (GOD) on polystyrene (PS) film surfaces modified with ozone aeration and UV irradiation (O3/UV) treatment were investigated. The total amount of GOD immobilized on the PS film modified with the O3/UV treatment in distilled water (PS-W film) was approximately twice as large as that on the film treated in an aqueous ammonia solution (PS-A film), whereas the specific activity of GOD on the PS-A film was four times higher than that on the PS-W film. In contrast, no enzymatic activity of GOD on the non-treated PS film was observed because of irreversible denaturation of the adsorbed GOD. We therefore conclude that the PS films modified by the O3/UV treatment in the aqueous media are effective in immobilizing GOD. 相似文献
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The direct electron transfer of glucose oxidase (GOD) was achieved based on the immobilization of GOD/colloidal gold nanoparticles on a glassy carbon electrode by a Nafion film. The immobilized GOD displayed a pair of well-defined and nearly reversible redox peaks with a formal potential (Eo ') of -0.434 V in 0.1 M pH 7.0 phosphate buffer solution and the response showed a surface-controlled electrode process. The dependence of Eo ' on solution pH indicated that the direct electron transfer reaction of GOD was a two-electron-transfer coupled with a two-proton-transfer reaction process. The experimental results also demonstrated that the immobilized GOD retained its electrocatalytic activity for the oxidation of glucose. So the resulting modified electrode can be used as a biosensor for detecting glucose. 相似文献
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将1-丁基-3-甲基咪唑四氟硼酸盐([BMIm][BF4])、N,N-二甲基甲酰胺(DMF)与葡萄糖氧化酶(GOD)的混合物修饰于三维有序大孔(3DOM)金膜电极上,构建了一种新型的葡萄糖传感器.固定的GOD在pH7.0的磷酸缓冲液(PBS)中展现出一对可逆性好的氧化还原峰,这归因于GOD的活性中心黄素腺嘌呤二核苷酸(FAD)的直接电化学行为.研究表明,离子液体(IL)、DMF以及3DOM金膜对GOD的直接电化学都起到了重要的作用.3DOM金膜修饰电极作为基底提高了酶的负载量,加速了GOD与电极表面的电子传递;IL的应用增加了固定GOD的电化学活性;DMF与IL、GOD的协同作用更好地保持了GOD的生物活性.固定在电极表面的GOD对葡萄糖显示出良好的催化性能,其检测线性范围为10~125nmol/L,检测限为3.3nmol/L(S/N=3),酶催化反应的表观米氏常数Km为0.018mmol/L. 相似文献
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A simple and effective glucose biosensor based on immobilization of glucose oxidase (GOD) in graphene (GR)/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface‐controlled process with the large electron transfer rate constant (ks) of 3.42±0.08 s?1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as‐prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14 mmol·L?1 with a detection limit of 0.03 mmol·L?1. Moreover, it displays a good reproducibility and long‐term stability. 相似文献
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A novel type of glucose sensor was fabricated based on a glucose oxidase (GOD)-N,N-dimethtylformamide (DMF)-[BMIm][BF4] composites modified three-dimensional ordered macroporous (3DOM) gold film electrode. The immobilized GOD exhibits a pair
of well-defined reversible peaks in 50 mM pH 7.0 phosphate buffer solutions (PBS), which could be attributed to the redox
of flavin adenine dinucleotide (FAD) in GOD. The research results show that ionic liquid ([BMIm][BF4]), DMF and 3DOM gold film are crucial for GOD to exhibit a pair of stable and reversible peaks. It is believed that the large
active area of 3DOM gold film can increase the amount of immobilized GOD. Simultaneously, the application of IL enhances the
stability of GOD and facilitates the electron transfer between GOD and the electrode. The synergetic effect of DMF can help
the GOD to maintain its bioactivity better. GOD immobilized on the electrode exhibits the favorable electrocatalytic property
to glucose, and the prepared sensor has a linear range from 10 to 125 nM with a detection limit of 3.3 nM at a signal-to-noise
ratio of 3σ. The apparent K
m (Michaelis- Menten constant) for the enzymatic reaction is 0.018 mM. 相似文献
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环糊精聚合物与苯醌的分子包合作用及其在酶电极中的应用 总被引:1,自引:1,他引:0
研究了水溶性环糊精预聚合物的存在对苯醌/氢醌体系在铂电极上氧化还原行为的影响, 根据伏安曲线讨论了该预聚合物与苯醌的分子包合作用。环糊精预聚合物与戊二醛缩聚反应而形成的不溶性聚合物膜用于葡萄糖氧化酶的固定化, 以制得新型的第二代葡萄糖电极。由于分子包合作用, 作为电子受体的苯醌在含酶的环糊精聚合物膜中具有较高的浓度, 从而加速了固定化酶的电子传递。测定了酶电极上BQ反应的动力学参数。 相似文献
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《Electroanalysis》2006,18(11):1131-1134
The direct electrochemistry of glucose oxidase (GOD) was revealed at a carbon nanotube (CNT)‐modified glassy carbon electrode, where the enzyme was immobilized with a chitosan film containing gold nanoparticles. The immobilized GOD displays a pair of redox peaks in pH 7.4 phosphate buffer solutions (PBS) with the formal potential of about ?455 mV (vs. Ag/AgCl) and shows a surface‐controlled electrode process. Bioactivity remains good, along with effective catalysis of the reduction of oxygen. In the presence of dissolved oxygen, the reduction peak current decreased gradually with the addition of glucose, which could be used for reagentless detection of glucose with a linear range from 0.04 to 1.0 mM. The proposed glucose biosensor exhibited high sensitivity, good stability and reproducibility, and was also insensitive to common interferences such as ascorbic and uric acid. The excellent performance of the reagentless biosensor is attributed to the effective enhancement of electron transfer between enzyme and electrode surface by CNTs, and the biocompatible environment that the chitosan film containing gold nanoparticles provides for immobilized GOD. 相似文献
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Makoto Yoshimoto Chihiro Iida Asuka Kariya Noriyuki Takaki Masaharu Nakayama 《Electroanalysis》2010,22(6):653-659
Glucose oxidase (GOD) was encapsulated in liposomes, and then the GOD‐containing liposomes were immobilized to a MnO2‐based multilayered nanocomposite film grown electrochemically. Oxidation of glucose took place on the encapsulated GOD in the manganese oxide film, and the generated H2O2 molecules were oxidized catalytically at high valent Mn sites (4+) in the film. Anodic currents due to reoxidation of the reduced Mn ions (3+) were in proportion to the concentration of glucose from 19.6 to 107.1 mM. Such a simple construction of biosensor is applicable to a variety of combinations of liposomal enzymes and substrates. 相似文献