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铜(铅)—Ferron—氯化四苯胂络合物吸附波研究 总被引:2,自引:1,他引:2
在HCl-六次甲基四胺介质中,铜(铅)-Ferron-氯化四苯胂络合物产生灵敏的络合吸附波。峰电位分别为-0.41V和-0.57V。峰电流与铜、铜浓度分别在0.0004-0.4μg/mL和0.002-0.8μg/mL之间呈线性关系,检出限分别为0.0002μg/mLCu和0.001μg/mLPb。研究了极谱波的性质和电极反应机理。用拟定的方法连续测定水样和食品中的铜、铜,结果满意。 相似文献
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本文研究了含氨介质中铬(Ⅲ,Ⅵ)-α,α-联吡啶-亚硝酸钠体系的电化学行为。建立了测定痕量铬的方法。测定铬的范围为0.004-0.028μg/mL和0.04-0.28μg/mL,检测下限为2.0×10^-^3μg/mL,用以测定生物样品中痕量铬,变异系数为9.8%。 相似文献
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焙烧富集分离—氢化物原子荧光法测定地质物料中痕量硒 总被引:2,自引:1,他引:2
本文研究了一种焙烧富集分离,氢化物-原子荧光法测定地质物料中痕量Se方法。系统地了Se富集分离条件,考查了30多种元素在焙烧前后对测定Se的影响。在选定的最佳实验条件下,方法检出下限为0.01μg/g和0.089μg/g,线性范围0.001-0.3μg/mL。样品中Se含量水平为0.036μg/g和0.089μg/g时的测量精度(RSD)分别为10%和5.8%。加标回收率为97-99%。采用本方法 相似文献
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在pH4.30的HAc-NaAc介质中,钪-间氯偶氮安替比林(m-CAA)-硫氰酸钾生成三元络合物,于-0.35V(vs.SCE)处出现一尖锐的极谱峰,峰电流与钪离子浓度在0.020~0.20μg/mL范围内呈线性关系,检出限为0.010μg/mL.用多种电化学方法研究了极谱波的性质及电极反应机理.方法用于矿样中钪的测定,结果满意 相似文献
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还原菌修饰碳糊电极研究及对微量金的测定 总被引:1,自引:1,他引:0
研究用细菌修饰的碳糊电极对金离子的响应特性,并应用该电极检测水溶液中的金离子,金离子家度在10-100μg/mL。呈线性关系,重现性为3.4%,检测限达1ng/mL,电极具有制备简单,灵敏诬高等优点。文中还讨论了金离子在还原菌修饰的碳糊电极上的还原机理。 相似文献
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脱乙酰壳多糖化学修饰电极测定铂的研究 总被引:8,自引:0,他引:8
用脱乙酰壳多糖修饰电极为工作电极,阳极溶出伏安法测定痕量铂。在pH=2 ̄3的KCl-HCl底液中,-0.3V富集2min,静止15s,以0.2V/s扫速阳极溶出,峰电位在-0.16V(vs.SCE),铂(Ⅳ)离子浓度在0.5 ̄5.0μg/mL范围内与峰高呈线性关系。富集10min后,可检测0.025μg/mL铂(Ⅳ)。该法用于贵金属矿样的测定,无需分离,结果满意。用循环伏安法、紫外光谱和拉曼光谱研 相似文献
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A standard ab initio (STO 3 G) study indicates, in distinction to theab initio molecular fragment results, that the preferred conformation of acetylcholin is gauche. 相似文献
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Raluca-Ioana Stefan-van Staden Amalia Gabriela Diaconeasa Carmen Cristina Surdu-Bob 《Analytical letters》2018,51(12):1927-1934
Acetylcholine and dopamine are neurotransmitters important for aging and brain pathology. Their assay in whole blood is essential for fast and early detection of neurodegenerative disorders. Therefore, polymeric textile covered with a thin layer of Ag was used to provide stochastic sensors modified with maltodextrins presenting different dextrose equivalence: Maltodextrin I (dextrose equivalence 13.0–17.0), and Maltodextrin II (dextrose equivalence 16.5–19.5). These stochastic sensors were used reliable for both qualitative and quantitative analysis of acetylcholine and dopamine in whole blood samples. Their sensitivity and selectivity were high, and they were reliable for the assay of dopamine and acetylcholine in whole blood samples, with recoveries higher than 98.00%, and relative standard deviations (%) values lower than 1.00%. 相似文献
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An acetylcholine sensor was constructed with acetylcholinesterase which was immobilized on a hydrogen ion-selective coated-wire electrode and fundamental properties of this sensor were investigated. Acetylcholine could be determined in the range 0.1–10 rum with response times of 3–10 min. The effects of pH and concentration of buffer solution on the determination and fluctuations in the data obtained with this sensor were also investigated. Possibilities for the practical use of this acetylcholine micro-sensor are suggested. 相似文献
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A novel method for highly sensitive detection of acetylcholine in royal jelly was proposed by using CE coupled with electrogenerated chemiluminescence (ECL). Acetylcholine, which could not react with Tris(2,2′‐bipyridine)ruthenium(II) to strengthen its ECL signals, decomposed into trimethylamine and strengthened the ECL signals sharply when it was heated to its melting point. This reaction needed no additional reagent and it was mild, simple, stable and rapid, without any side reaction. By combining the above process with CE separation technique, trimethylamine in royal jelly was completely separated from interfering substances and was successfully detected within 4 min. The limit of detection for acetylcholine was found to be 6.3×10?8 g/mL with a signal‐to‐noise ratio of 3:1. Acetylcholine in the royal jelly was detected to be 912±58 μg/g. The recoveries of acetylcholine chloride in the sample were in the ranges of 92–106%. The coefficients of variation for intra‐day and inter‐day reproducibility were equal to or less than 4.9 and 6.8%, respectively. 相似文献
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Keeley L. Baker Fiachra B. Bolger Michelle M. Doran John P. Lowry 《Electroanalysis》2019,31(1):129-136
A choline biosensor was characterised in detail to determine the effects of physiologically relevant parameters on the ability of the sensor to reliably detect neurochemical changes in choline. This first generation Pt‐based polymer enzyme composite sensor displayed excellent shelf‐life and biocompatibility with no significant decrease in choline sensitivity observed following 14 days of storage dry, or in ex‐vivo rodent brain tissue. However, subjecting the sensor to repeated calibrations and storage over the same period resulted in significant decreases (20–70 %) due to enzyme denaturation associated with the repeated calibration and storage cycles. Potential interference signals generated by the principal electroactive interferents present in the brain were minimal; typically <1 % of the choline current response at in vivo levels. Additionally, changing temperature over the physiologically relevant range of 34–40 °C had no effect on sensitivity, while increasing pH between 7.2 and 7.6 produced only a 5 % increase in signal. The limit of detection of the sensor was in the low μM range (0.11±0.02 μM), while the in vitro response time was determined to be less than the solution mixing time and within ca. 5 s, suggesting potential sub‐second in vivo response characteristics. Finally, the sensor was implanted in the striatum of freely moving rats and demonstrated reliable detection of physiological changes in choline in response to movement, and pharmacological manipulation by injection of choline chloride. 相似文献
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《Electroanalysis》2006,18(5):465-470
Nickel and platinum find extensive use in preparation of biosensors. In the present work, Ni/Pt alloy was plated on graphite to make acetylcholine sensor. The microstructure, surface composition and electrochemical performance of the electrode was analyzed by different techniques. The sensing performance was evaluated by cyclic voltammetry. The prepared alloy plate exhibited very good linear relationship between acetyl choline concentration and response current. The sensitivity and reproducibility of the prepared electrode were found better than other nickel electrodes reported. 相似文献
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To detect a low level of acetylcholine (Ach) output in the dialysate of rat frontal cortex, osmium-peroxidase redox polymer modified gold-ring disk electrode (Os-HRP-RDE) was fabricated in high-performance liquid chromatography (HPLC)-electrochemical detection (ECD). In comparison with platinum electrode, the sensitivity of acetylcholine detection by Os-HRP-RDE was stable during a day, the coefficient of variation was 0.95%, and the decrease of the sensitivity for 1 week was 5.4%. The Os-HRP-RDE could detect lower than 3 fmol of acetylcholine. The present technique, in the absence of acetylcholinesterase (AchE) inhibitor, is useful to facilitate physiological investigations of cholinergic neuronal activity in the brain. 相似文献
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A biodegradable polymer drug-delivery system has been developed for the selective localized application of agents to brain parenchyma. The copolymer of poly[bis(p-carboxyphenoxy)propane] anhydride and sebacic acid (PCPP–SA) was impregnated with [3H]-acetylcholine (ACh) to form 1–5 μm microspheres. Drug-loaded microspheres were implanted into hippocampus bilaterally in 25 rats, and brain sections processed for autoradiography in groups of five animals at 2, 5, 10, 20 and 40 days, respectively. By densitometric analysis, the concentration of radiolabelled ACh in polymer and adjacent hippocampus rapidly decreased between 2 and 5 days, after which a gradual decrease in [3H]-ACh was observed up to 40 days. Between 2 and 40 days the concentration of radiolabelled ACh was reduced by 25.8% in polymer matrix and 40.1% in hippocampus. The spread of [3H]-label into adjacent brain parenchyma showed a similar temporal relationship, with initially wider dispersion at 2 days (44±3 μm), then a linear decrease in dispersion over the remaining period (10±0.9 μxm at 40 days), suggesting bulk flow of the radiolabel into hippocampus. Brain parenchyma showed only a minimal inflammatory reaction to the polyanhydride implants over all time periods. Polyanhydrides can provide localized continuous release of ACh to brain parenchyma, and may potentially be used to deliver various agents to brain in a number of clinical and experimental applications. 相似文献
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The title compounds, the P(3)‐axially and P(3)‐equatorially substituted cis‐ and trans‐configured 7‐benzyl‐3‐fluoro‐2,4‐dioxa‐7‐aza‐3‐phosphadecalin 3‐oxides (=7‐benzyl‐3‐fluoro‐2,4‐dioxa‐7‐aza‐3‐phosphabicyclo[4.4.0]decane 3‐oxides=5‐benzyl‐2‐fluorohexahydro‐4H‐1,3,2‐dioxaphosphorino[5,4‐b]pyridine 2‐oxides) were prepared (ee>99%) and fully characterized (Schemes 2 and 4). The absolute configurations were established from that of their precursors, the enantiomerically pure cis‐ and trans‐1‐benzyl‐3‐hydroxypiperidine‐2‐methanols which were unambiguously assigned. Being configuratively fixed and conformationally constrained phosphorus analogues of acetylcholine, they mimic rotamers of acetylcholine and are suitable probes for the investigation of molecular interactions with acetylcholinesterase. As determined by kinetic methods, the compounds are irreversible inhibitors of the enzyme displaying significant stereoselectivity. 相似文献