基于螺旋相位调制的非相干全息点扩散函数研究

分析了菲涅耳非相干相关全息(Fresnel incoherent correlation holography,FINCH)系统中纯相位空间光调制器(spatial light modulator,SLM)加载螺旋相位掩模时的点扩散函数.以氙灯为照明光源搭建了FINCH系统,电荷耦合器记录的点源全息图与点扩散函数模拟结果一致.采用该系统分别在SLM上加载双透镜掩模和螺旋相位调制双透镜掩模两种情况下对分辨率板和非染色洋葱细胞成像,给出了成像对比结果.结果表明:采用螺旋相位调制的FINCH系统可以在几乎不牺牲分辨率的情况下提高图像的边缘对比度;同样,对相位物体也可以实现图像的边缘提取和识别.该方法在实时监测活细胞的分裂、形变等方面具有重要应用前景.     

基于空间光调制器的非相干数字全息单次曝光研究

菲涅耳非相干相关全息术(Fresnel incoherent correlation holography,FINCH)利用在空间光调制器(spatial light modulator,SLM)上加载双透镜模式对同一物点光分束自相干,并通过改变加载的相位因子得到不同的相移全息图.本系统利用SLM可分区编码调制特性,将FINCH成像中SLM上分三次加载的0°,120°,240°相位双透镜掩模各提取1/3组成一幅复合相移模式加载,并研究了三种相位分布方式对FINCH成像质量的影响.结果表明:三个相位在SLM上分布间隔越大,再现像越清晰.在此基础上,提出了一种新的掩模加载方式,在SLM加载透镜阵列,每一个相位因子对应一个双透镜,具有一个光轴.实验表明,通过这种加载方式,通过SLM后形成的三个相移图能够一次在电荷耦合器上记录,并且三个相移图不重叠,然后通过MATLAB编程计算将不同相移角度的全息图分别提取出来,通过三步相移计算合成一幅包含有物光波的复值全息图,最后通过数值再现算法重建待测样品.此系统可用于对光源相干性较低的实时成像系统,也为微小形变测量、动态物体的观测提供了新方法,为非相干数字全息术的发展提供了新思路.     

Incoherent digital holographic spectral imaging with high accuracy of image pixel registration

Fresnel incoherent correlation holography(FINCH) is a unique three-dimensional(3 D) imaging technique which has the advantages of scanning-free,high resolution,and easy matching with existing mature optical systems.In this article,an incoherent digital holographic spectral imaging method with high accuracy of spectral reconstruction based on liquid crystal tunable filter(LCTF) and FINCH is proposed.Using the programmable characteristics of spatial light modulator(SLM),a series of phase masks,none of whose focal lengths changes with wavelength,is designed and made.For each wavelength of LCTF output,SLM calls three phase masks with different phase constants at the corresponding wavelength,and CCD records three holograms.The spectral images obtained by this method have a constant magnification,which can achieve pixel-level image registration,restrain image registration errors,and improve spectral reconstruction accuracy.The results show that this method can not only obtain the 3 D spatial information and spectral information of the object simultaneously,but also have high accuracy of spectral reconstruction and excellent color reproducibility.     

Selective edge enhancement in three-dimensional vortex imaging with incoherent light

We demonstrate a new imaging method enabling a selective edge contrast enhancement of three-dimensional amplitude objects with spatially incoherent light. The imaging process is achieved in a spiral modification of Fresnel incoherent correlation holography and uses a vortex impulse response function. The correlation recordings of the object are acquired in a one-way interferometer with the wavefront division carried out by a spatial light modulator. Two different methods based on applying a helical reference wave in the hologram recording and a digital spiral phase modulation in image reconstruction are proposed for edge enhancement of amplitude objects. Results of both isotropic and anisotropic spiral imaging are demonstrated in experiments using an LED as an incoherent source of light.     

图像传感器像素化效应对菲涅耳非相干关联全息分辨率的影响

作为复光场显微成像的一种新技术,菲涅耳非相干关联全息术(Fresnel incoherent correlation holography,FINCH)因其非相干光记录的特点在近年来受到关注.FINCH作为一种新型非相干全息系统,如何设计光路实现其最佳的分辨率是一个关键问题.然而,针对这个问题的讨论,目前已有文献存在不同的观点,有关FINCH最佳分辨率的成像条件仍有待研究.全息图有效孔径大小是决定全息成像系统分辨率的重要因素,在FINCH系统中,全息记录距离的变化则会引起全息图有效孔径发生变化,全息图的有效孔径大小不仅与光路各元件的孔径有关,还与相干光波相互干涉叠加区域的面积以及图像传感器的像素间距等因素有关.本文基于波动光学理论,结合FINCH全息图的波带结构特征,研究了FINCH全息图的有效孔径.研究发现数字全息记录相机的像素化特性是影响FINCH成像分辨率的决定性因素,并进一步通过数值模拟和光学实验验证了理论分析结果:全息图记录距离(Z_h)等于空间光调制器加载的衍射透镜焦距(f_d)时,FINCH系统的再现像将会达到最佳横向分辨率,且分辨率随成像距离|Z_h-f_d|的增大而降低.     

基于位相变更的非相干数字全息自适应成像

菲涅耳非相干数字全息作为一种非扫描的三维成像技术具有其独特的优势,但其成像过程中会受到各种像差的影响,导致成像分辨率、再现像的质量降低.为了解决这一问题,可以结合适当的自适应光学技术对波前像差进行探测和校正.位相变更是一种基于两幅具有已知位相差的强度图像实现波前探测和像差校正的技术.本文发展了基于位相变更的非相干数字全息自适应成像技术,不需要引入引导星,利用全息记录过程中的两幅相移全息图,实现波前像差的探测.本文给出了所发展技术的数值仿真和实验结果,结合位相变更算法求解出系统像差的位相分布,将像差的共轭位相加载到光瞳面上,在全息图记录的同时校正像差,从而提高重建像的质量.     

基于液晶空间光调制器的全息显示

在传统的纯相位全息显示系统中, 一般基于快速傅里叶变换(FFT)算法来计算相位全息图, 在FFT的计算中需要遵循Nyquist采样定理, 因此, 重建图像的尺寸往往受限于空间光调制器的固定采样率. 这个限制可以通过卷积算法或者两步菲涅耳衍射算法来解决, 但是需要使用多个FFT的计算, 导致计算量增大. 鉴于此, 提出了一种基于透镜的纯相位全息图计算方法. 在全息图的计算中, 通过透镜的成像原理建立一个采样率可变的虚拟全息面, 通过调节相应的距离参数使得在全息图的计算中可以任意调节原始图像的采样率, 摆脱了传统方法中液晶空间光调制器带宽积对重建图像尺寸的限制, 并且这种算法只需使用一次FFT就能达到变采样率的衍射计算, 大幅提高了全息图的计算速度. 数值模拟及光学实验结果证明了此方法可以在全息显示光学系统中清晰地重建不同尺寸的图像. 同时该系统可以有效地消除由空间光调制器的像素化结构带来的零级衍射.     

三维物体多重菲涅耳计算全息水印与无干扰可控重建方法

提出了一种基于三维物体的多重菲涅耳计算全息水印方法.将水印信号作为虚拟三维物体的层面,首先结合分区复用层析法和菲涅耳双随机相位编码方法产生复噪声形式的水印信号;然后对水印信号的频谱作共轭对称处理实现实值编码;为减小对宿主全息图数字重建的影响,将水印信号的频谱设置于对宿主数字重建影响小的频谱非感兴趣区域;编码后的信号以一定强度叠加于宿主全息图,水印信号恢复无需原始宿主全息图信息,可实现盲提取,对宿主全息图重建像面的二维码可扫描识别.仿真测试结果表明,所提出的方法具有较好的透明性和稳健性,在宿主全息图遭受滤波、JPEG(联合图像专家小组)压缩、高斯噪声、剪切、旋转等各种攻击的情况下,不论对宿主还是水印信号仍具有良好的数字重建质量,对重建像面的二维码仍可扫描识别;而重建像面水印信号的无干扰可控重建后处理操作解决了不同层面水印信号之间的衍射干扰问题,提高了水印信号的重建质量.虚拟光学手段的应用丰富了水印信号设计方法并提升了算法的安全性.     

基于菲涅耳波带板扫描全息术的光学层析成像

基于光学扫描全息术原理和编码孔径层析成像技术,提出了一种三维物体层析成像技术——菲涅耳波带板扫描全息术。该技术通过将物体的光强分布函数与菲涅耳波带板的光强分布函数进行卷积运算来得到物体的扫描全息图,通过相关解卷积的方法来进行扫描全息图的数字重建。依据该技术原理设计了实验系统,系统结构简单紧凑,具有三维物体层析成像功能。提出用数字高通滤波器来减少重建图像的背景噪声。计算机仿真及对实物的扫描记录和重建实验结果表明,该技术原理正确,用于三维物体层析成像是可行的,用数字高通滤波的方法提高重建图像的对比度是有效的。     

通过保留相位进行全息图信息压缩的方法

从全息图的特点出发,研究了全息图的记录和再现原理,提出了一种对离轴菲涅耳全息图信息压缩的方法。对全息图进行傅里叶变换,滤除零级噪声及物光波的共轭波前,仅仅保留物光波的相位信息,而后以采用较低的采样频率对全息图重新抽样,从而大大减少了数字全息图的信息冗余。压缩后的全息图也可以获得良好像质的再现像。理论和实验均证明了该方法的可行性,为全息图的信息存贮与传输提供一条新的途径。     

Single-exposure two-dimensional superresolution in digital holography using a vertical cavity surface-emitting laser source array

We present a new implementation capable of producing two-dimensional (2D) superresolution (SR) imaging in a single exposure by aperture synthesis in digital lensless Fourier holography when using angular multiplexing provided by a vertical cavity surface-emitting laser source array. The system performs the recording in a single CCD snapshot of a multiplexed hologram coming from the incoherent addition of multiple subholograms, where each contains information about a different 2D spatial frequency band of the object's spectrum. Thus, a set of nonoverlapping bandpass images of the input object can be recovered by Fourier transformation (FT) of the multiplexed hologram. The SR is obtained by coherent addition of the information contained in each bandpass image while generating an enlarged synthetic aperture. Experimental results demonstrate improvement in resolution and image quality.     

全息再现的分析

本文讨论了无像差再现像存在的条件及不失真的条件,并具体指出球面参考光形成的菲涅耳全息图可有四个既无像差又不失真的再现像。总结出成像的规律便于人们真实地再现原物。     

X-ray interferometric Fourier holography

An X-ray interferometric Fourier holography was proposed and theoretically investigated. X-ray Interferometric Young fringes and the reconstruction of an object image were investigated by the Fourier transform method. It was shown that on the output surface of the analyzer crystal (the third plate of the interferometer) the interference pattern of two slits gives X-ray interferometric Young fringes. An expression for the period of X-ray interferometric Young fringes was obtained. The subsequent reconstruction of the slit image as an object is conducted by means of the Fourier transform method of intensity distribution on the hologram. Three methods for reconstruction of the function of complex transmission of the object are presented: an analytical one–the approximate method, the iteration method and the step-by-step approach. As examples a recording of X-ray interferometric Fourier hologram and the reconstruction of the function of complex amplitude transmission of a beryllium circular cylinder are given.     

菲涅耳非相干数字全息大视场研究

菲涅尔非相干相关全息术(Fresnel incoherent correlation holography, FINCH)通过空间光调制器(spatial light modulator, SLM)将来自物点的光波分解为曲率半径不同的两束自相干光,干涉条纹由CCD记录.由于受限于SLM与CCD的像素数目及像素尺寸, FINCH技术与光学全息术相比记录视场要小得多.本文通过对FINCH系统的记录过程进行理论分析,给出了SLM所能记录的视场角,说明通过调控加载在SLM上的双透镜光轴中心,能够扩大SLM的有效直径从而将SLM的有效记录范围增大2.77倍,有效扩大了系统的记录视场.搭建了非相干光反射式数字全息记录系统并对理论分析进行了实验验证,结果表明:在SLM上依次加载不同光轴中心位置的双透镜掩模进行FINCH记录及再现,将得到的各子图像拼接融合可以得到高分辨率大视场图像,为菲涅尔非相干全息术在高分辨大视场显微成像的进一步应用提供了有力支撑.     

Resolution improvement of digital holographic images based on angular multiplexing with incoherent beams in orthogonal polarization states

We present a method to improve the resolution of digital holographic images based on angular multiplexing with incoherent beams from two orthogonal polarized components of natural light. Two incoherent subholograms are synchronously recorded by two pairs of incoherent object waves and reference waves with orthogonal polarization states, in which the object is illuminated by two incoherent beams from different directions. The increase in resolution is obtained through phase correction and superposition of two reconstructed object waves. Experimental results show that the resolution and quality of the reconstructed image can be effectively improved.     

X-ray interferometric Fresnel holography

The X-ray interferometric Fresnel holography was proposed and theoretically investigated. It was shown that under definite conditions the recorded interference pattern formed at the output surface ofthe analyzer crystal (the third block of interferometer) is the X-ray interferometric hologram (generally the Fresnel one) of the object under investigation. Further reconstruction of an image is performed with the help of visible light or a numerical method. As an example the recording of a Fresnel hologram of the simplest case of one-dimensional object, a narrow slit, and the reconstruction of an image by means of visible light were considered. This method may be used in X-ray microscopy and realized using synchrotron sources ofX-ray radiation, as well as X-ray free electron lasers.     

Digital spatially incoherent Fresnel holography

We present a new method for recording digital holograms under incoherent illumination. Light is reflected from a 3D object, propagates through a diffractive optical element (DOE), and is recorded by a digital camera. Three holograms are recorded sequentially, each for a different phase factor of the DOE. The three holograms are superposed in the computer, such that the result is a complex-valued Fresnel hologram. When this hologram is reconstructed in the computer, the 3D properties of the object are revealed.     

Fluorescence multicolor hologram recorded by using a macrolens array

We present a new efficient method for obtaining multicolor digital Fresnel holograms of three-dimensional (3-D) objects emitting incoherent fluorescent light. For each emitted fluorescent light wavelength, a single monochromatic digital modified Fresnel hologram of the 3-D scene is generated. Using a macrolens array, only nine projections of the 3-D scene are captured in a single camera shot and are then digitally processed to yield the hologram. The 3-D reconstructed images from all the monochromatic holograms are composed into a single multicolor image of the 3-D scene. The proposed holographic technique is demonstrated by experiments.     

Geometrical optics in Fresnel quantum holography

The geometrical optics in Fresnel quantum holography for quantum entangled two-photon source and classical thermal light source based on the second-order correlation measurement has been discussed. We theoretically prove that the Fresnel quantum hologram of the detected object can be obtained through second-order correlation measurement and the optical reconstruction process can be accomplished by making use of a point light source.     

Aspects of nanometer scale imaging with extreme ultraviolet (EUV) laboratory sources

Imaging systems with nanometer resolution are instrumental to the development of the fast evolving field of nanoscience and nanotechnology. Decreasing the wavelength of illumination is a direct way to improve the spatial resolution in photon-based imaging systems and motivated a strong interest in short wavelength imaging techniques in the extreme ultraviolet (EUV) region. In this review paper, various EUV imaging techniques, such as 2D and 3D holography, EUV microscopy using Fresnel zone plates, EUV reconstruction of computer generated hologram (CGH) and generalized Talbot self-imaging will be presented utilizing both coherent and incoherent compact laboratory EUV sources. Some of the results lead to the imaging with spatial resolution reaching 50 nm in a very short exposure time. These techniques can be used in a variety of applications from actinic mask inspection in the EUV lithography, biological imaging to mask-less lithographic processes in nanofabrication.