用全反射方法测量离体动物组织的折射率

利用全反射原理测量了新鲜离体动物组织在650 nm处的折射率.选择了鸡胸肉、猪肌肉、牛肌肉、羊肉、猪肝脏和猪肾脏六种类型的组织作为研究对象,将其制成2 mm厚的切片进行测量,折射率的测量结果范围在1.384 52~1.418 26.特别是对其中肌肉纤维取向明显的鸡胸肉、猪肌肉、牛肌肉,分别测量了肌肉纤维在平行和垂直棱镜与生物组织界面两种情况下的折射率.结果表明,测量的肌肉纤维垂直界面时的折射率要大于平行时的结果.     

景观湖水质的三维荧光指纹

荧光光谱法灵敏度高、选择性强、测量快速便捷,而三维荧光光谱更有信息丰富的优点。荧光光谱与水样具有一一对应的关系,就像水样指纹一样,被称为“水质荧光指纹”。 该文采用三维荧光光谱法研究了北京两个相邻景观湖四季的水质变化。两湖湖水均具有明显的类蛋白质和类腐殖酸的荧光,且类蛋白质的荧光强度较强。荧光强度与自然和人为活动的相关性表明,两湖湖水水质随季节变化明显,春夏季节水质相对较好,秋冬季节水质相对较差;以游船为主的娱乐湖的水质主要受藻类和植物生长等自然因素的影响,而种植等人为因素对植物观赏湖的水质的影响十分重要。由此可见,湖水水质的变化是由湖的功能决定的。在春秋人工清理藻类和植物的残余有利于保护湖体水质。     

The radioprotective and prophylactic properties of biologically active substances prepared by supercritical fluid extraction from pine shoots

The radioprotective properties of a substance from pine shoots prepared by supercritical fluid extraction with carbon dioxide were studied for a model of an experimental bone-marrow radiation sickness form. Experiments were performed for white nonlinear mice. The CO₂ extract of pine shoots was shown to possess radioprotective properties. The extract was subjected to pre-clinical tests for Wistar line male rats with respect to its antiatherogenic and antioxidative action in the creation of experimental hyperlipemia.     

Lysimeter Investigations on the Effect of Winter Catch Crops and Weeded Fallow on the N-Dynamics in a Sandy Treposol Soil of Northeast Germany

Abstract

Lysimeter experiments (soil: sandy treposol, from the region “Havelländisches Luch”, Brandenburg, Germany) with application of ¹⁵N labelled fertilizer (80 kg N per ha as ¹⁵NH₄ ¹⁵NO₃, 10 at.-%¹⁵N exc.; for simulating mineralization in the early autumn period) were carried out to determine to what extent the amount of mineral- N was temporary conserved by winter catch crops, taken up subsequently in the vegetation periods by following crops, taken by subsequently in the vegetation periods by following crops, or percolated in the leaching water, respectively. The results were as follows:

1) Until winter or spring respectively, the catch crop uptake rates of applied mineral-N were 32% for phacelia (Phacelia tanacetifolia BENTH.), 25% for winter rape (Brassica napus L. cv. ‘AKELA’), and 16% for white mustard (Sinapis alba L.).

2) In the year after, following maize incorporated from 2.1 to 4.5% of the fertilizerborne N. The following plant community of fallow took up from 0.2 to 0.5% N originating from the fertilizer-N.

3) In comparison with the catch crops, N-leaching losses under fallow conditions were highest and equivalent to 17% of the applied fertilizer-N amount. In contrast to 3% of white mustard, phacelia and winter rape reduced N-leaching losses to 0.2 and 0.3% of the applied fertilizer-N amount.

4) In spring of the first year after the beginning of investigations, N-leaching losses were highest under fallow conditions and white mustard cultivation. Thus, the amounts of nitrate losses would exceed the EU limit for drinking water.

5) Three years after the investigations had been started, 10% (white mustard) and 20% (fallow) of the applied fertilizer-N was still found in th lysimeter soil.     

Organization and quantification of the collagen fibers in bone formation during orthodontic tooth movement

The organic matrix of alveolar bone is composed fundamentally of type I collagen. Polarized light microscopy provides unique information about the structure, composition and polymerization degree of a variety of organic and inorganic tissues that is not available with other techniques. The aim of this research was to compare two methodologies of polarized light analysis for collagen organization in bone formation during orthodontic tooth movement and determined maturity of collagen over the time. Thirty Wistar rats were euthanized 3, 7 and 14 days after the NiTi unilateral closed-coil spring was stretched between the upper right first molar and the incisors. The control consisted by contra-lateral site. The first molar area was fixed, decalcified and histologically processed using picrosirius pigment. The collagen birefringence of bone turnover was analyzed by color percentage and phase retardation. We observed an increase in collagen fiber organization over time with two methodologies. The Pearson coefficient correlation indicated a strong relationship (0.76) among the two polarized light analyses. In summary, there is collagen maturation over 3, 7 and 14 days. We successfully evaluated the molecular organization, arrangement, degree of polymerization and maturation process of collagen fibers in bone turnover through color percentages and phase retardation.     

Genomic and neural analysis of the estradiol-synthetic pathway in the zebra finch

Background  

Steroids are small molecule hormones derived from cholesterol. Steroids affect many tissues, including the brain. In the zebra finch, estrogenic steroids are particularly interesting because they masculinize the neural circuit that controls singing and their synthesis in the brain is modulated by experience. Here, we analyzed the zebra finch genome assembly to assess the content, conservation, and organization of genes that code for components of the estrogen-synthetic pathway and steroid nuclear receptors. Based on these analyses, we also investigated neural expression of a cholesterol transport protein gene in the context of song neurobiology.     

Daily variation of radon gas and its short-lived progeny concentration near ground level and estimation of aerosol residence time

Atmospheric concentrations of radon(~(222)Rn) gas and its short-lived progenies~(218)Po,~(214)Pb, and~(214)Po were continuously monitored every four hours at the ground level in Jeddah city, Kingdom of Saudi Arabia. The measurements were performed three times every week, starting from November 2014 to October 2015. A method of electrostatic precipitation of positively charged~(218)Po and ~(214)Po by a positive voltage was applied for determining~(222)Rn gas concentration. The short-lived~(222)Rn progeny concentration was determined by using a filter holder connected with the alpha-spectrometric technique. The meteorological parameters(relative air humidity, air temperature, and wind speed) were determined during the measurements of~(222)Rn and its progeny concentrations.~(222)Rn gas as well as its short-lived progeny concentration display a daily and seasonal variation with high values in the night and early morning hours as compared to low values at noon and in the afternoon. The observed monthly atmospheric concentrations showed a seasonal trend with the highest values in the autumn/winter season and the lowest values in the spring/summer season. Moreover, and in parallel with alphaspectrometric measurements, a single filter-holder was used to collect air samples. The deposited activities of~(214)Pb and the long-lived~(222)Rn daughter ~(210)Pb on the filter were measured with the gamma spectrometric technique. The measured activity concentrations of~(214)Pb by both techniques were found to be relatively equal largely. The highest mean seasonally activity concentrations of ~(210)Pb were observed in the autumn/winter season while the lowest mean were observed in the spring/summer season. The mean residence time(MRT) of aerosol particles in the atmospheric air could be estimated from the activity ratios of ~(210)Pb/~(214)Pb.     

Seasonal variation of radon-222 concentrations in specific locations in Jordan

Previously calibrated passive detectors (CR-39) and an active radon device (Radon Monitor RM3) were used to study seasonal variation of radon-222 concentration levels inside and outside specific locations in Jordan. The study sites were located in an area that used to be an old phosphate mine. We found that the maximum value of radon concentration in air inside the dwellings, as measured by the passive dosimeters, was 1532.9 Bq/m³ during the winter season, and the minimum one was 46.3 Bq/m³ during fall season. While the highest and lowest readings of the active monitor were 892 and 4 Bq/m³ during fall and summer seasons, respectively. The radon concentration in soil ranges from 0.2 kBq/m³ in spring to 37.8 kBq/m³ in fall.     

T1rho dispersion of rat tissues in vitro.

The purpose of this study was to demonstrate T1rho dispersion in different rat tissues (liver, brain, spleen, kidney, heart, and skeletal muscle), and to compare the 1/T1rho data to previous 1/T1 data and magnetization transfer of rat tissues at low (0.1 T) B0 field. The 1/T1rho dispersion showed a fairly similar pattern in all tissues. The highest 1/T1rho relaxation rates were seen in liver and muscle followed by heart, whereas the values for spleen, kidney, and brain were quite similar. Compared to 1/T2 relaxation rate, the greatest difference was seen in liver and muscle. The rank order 1/T1rho value at each locking field B1 was the same as the transfer rate of magnetization from the water to the macromolecular pool (Rwm) for liver, muscle, heart, and brain. The potential value T1rho imaging is to combine high T1 contrast of low field imaging with the high signal to noise ratio of high static field imaging. When the T1rho value for a given tissue is known, the contrast between different tissues can be optimized by adjusting the locking time TL. Further studies are encouraged to fully exploit this. Targets for more detailed research include brain infarct, brain and liver tumors.     

Atmospheric temperature time series analysis at Mt. Cimone Observatory, Italy, 1946–1991

Summary Observations of atmospheric temperature have been uninterruptedly carried out at Mt. Cimone (2165 m s.l.) since January 1946. Four decades and a half of homogeneous measurements are presented in this paper giving particular emphasis to long-term change: in the yearly temperature averages there is no evidence for secular trend as well as in the winter and summer temperature averages. On the contrary the spring averages show a decreasing long-term trend, while the autumn averages reveal an increasing long-term trend. We find, for the spring season, a global decrease of about −1°C and, for the autumn season, a global increase of about +1°C. A temperature correlation analysis between Mt. Cimone and an area-box of about (200×700) km², exceeding the Po Valley, just to the North of Mt. Cimone, is presented. Results show how long-term temperature change, representing the mentioned area, could be powerfully monitored by operating a single station as Mt. Cimone.     

Changes of relaxation times T1 and T2 in rat tissues after biopsy and fixation

NMR spectroscopical measurements of relaxation times were conducted on muscle, intestine, fatty tissue and cerebral cortex and white matter of the rat at various time intervals following removal of the tissue. It appeared that most tissues can be stored at 4 degrees C up to 24 hours without noticeable effects on NMR relaxation parameters. Exceptions are the T2 of muscle and the T1 and T2 of intestine, which tended to change in the first hour after biopsy. Relaxation parameters change considerably after fixation of the tissues. Therefore the effects of fixation have to be taken into account when carrying out NMR measurements on fixed tissues.     

General relativistic effects in the structure of massive white dwarfs

In this work we investigate the structure of white dwarfs using the Tolman–Oppenheimer–Volkoff equations and compare our results with those obtained from Newtonian equations of gravitation in order to put in evidence the importance of general relativity (GR) for the structure of such stars. We consider in this work for the matter inside white dwarfs two equations of state, frequently found in the literature, namely, the Chandrasekhar and Salpeter equations of state. We find that using Newtonian equilibrium equations, the radii of massive white dwarfs (\(M>1.3M_{\odot }\)) are overestimated in comparison with GR outcomes. For a mass of \(1.415M_{\odot }\) the white dwarf radius predicted by GR is about 33% smaller than the Newtonian one. Hence, in this case, for the surface gravity the difference between the general relativistic and Newtonian outcomes is about 65%. We depict the general relativistic mass–radius diagrams as \(M/M_{\odot }=R/(a+bR+cR^2+dR^3+kR^4)\), where a, b, c and d are parameters obtained from a fitting procedure of the numerical results and \(k=(2.08\times 10^{-6}R_{\odot })^{-1}\), being \(R_{\odot }\) the radius of the Sun in km. Lastly, we point out that GR plays an important role to determine any physical quantity that depends, simultaneously, on the mass and radius of massive white dwarfs.     

西藏地区近地晴天大气电场气象效应和时间变化研究

基于西藏羊八井宇宙线观测站在2006年3月至2011年6月期间记录的近地大气电场数据, 分析研究了该地区近地晴天大气电场气象效应和时间变化特征. 气象效应分析结果显示, 该地区近地晴天大气电场与三个气象参量(大气压强、温度和相对湿度)的长时间变化趋势基本一致, 并有明显的季节效应. 冬春季, 大气电场强度整体水平相对较低, 约为0.14 kV/m; 夏秋季, 电场强度水平相对较高, 为0.18 kV/m左右. 近地晴天大气电场强度与大气温度间的线性相关性最强, 拟合相关系数达到0.89; 与大气压强和相对湿度间的线性相关性相对较弱, 拟合相关系数依次为0.43和0.53. 傅里叶分析结果表明: 晴天大气电场时间变化受太阳日周期、半太阳日周期及其三、四次谐波分量调制作用, 调制强度依次减弱. 西藏地区近地晴天大气电场日变化特征呈大陆简单型, 即双峰双谷. 主、次峰谷分别出现在白天和夜间, 主峰谷出现的早晚因季节不同略有差异, 次峰谷出现的早晚因季节不同差异相对较大.     

Synchrotron radiation X-ray fluorescence used to studying the migration of chemical elements in biogeochemical food chains

Synchrotron radiation X-ray fluorescence makes it possible to determine the multielemental composition of atmospheric aerosols and other components of biogeochemical chains. From these data, a close interrelation between the concentration of elements in atmospheric aerosols and tissues (liver, bone tissues) of small mammals of the Central Urals and in tissues (hair, bone tissues) of wild animals of Gornyi Altai has been revealed by correlation analysis. A similar interrelation has been established for the elemental composition of atmospheric aerosols and food, the elemental composition of atmospheric aerosols, and other components of biogeocenosis (plants, mushrooms and soil). It is assumed that atmospheric aerosols play an important role in the global cycling of chemical elements.     

Attenuation of ultrasound in homogenates of bovine skeletal muscle and other tissues

The attenuation of ultrasound in homogenates of bovine skeletal muscle and suspensions of myofibrils was measured over the frequency range 1.5-7 MHz, and found to be proportional to protein concentration in both. In the homogenates it varied with frequency and temperature in a similar way to the attenuation in post rigor muscle tissue; myofibrils showed a higher frequency dependence. The attenuation in homogenates of bovine muscle, liver and kidney and in suspensions of myofibrils was measured over the pH range 3.5-13, and each showed a peak at about pH 11.5. This was thought to be due to a proton transfer process between NH3+ groups on the tissue proteins and OH- ions in the suspending fluid. A substantial peak at about pH 5 in the muscle and myofibril suspensions was not observed in homogenates of liver and kidney and was thought to be due to components of muscle that are absent from the other tissues. Myofibrils suspended in percoll solution of density 1.05 g cm-3, chosen to match approximately the density of the myofibrils, showed a slightly lower attenuation over the pH range 5-7, but a pH dependence similar to that of the myofibrils suspended in saline. The difference in the attenuations may be interpreted as the viscous component of the attenuation due to relative motion between the myofibril and its surrounding saline. The peak at pH 5 did not, however, appear to be due to the viscous loss mechanism peaking due to maximum shrinkage (and therefore maximum density) of the myofibril near this pH.(ABSTRACT TRUNCATED AT 250 WORDS)     

Tensor Expressions for Solving Einstein's Equations by the Method of Sequential Approximation

The main aim of this paper is to develop a mathematical tool for General Relativity (GR). For this purpose useful tensor expressions have been worked out, which considerably ease various calculations using the sequential approximation in Einstein's GR. Based upon these expressions, compact and explicit formulae have been worked out for the covariant and contravariant components of the metric tensor and its determinant.     

T1rho Dispersion profile of rat tissues in vitro at very low locking fields

The purpose of this study was to show the T(1rho) dispersion profile in various rat tissues (liver, brain, spleen, kidney, heart and skeletal muscle) at low (0.1 T) B(0) field at very low locking field B1, starting from 10 microT. The T(1rho) dispersion profile showed a quite similar pattern in all tissues. The highest R(1rho) relaxation rates were seen in the liver and muscle followed by the heart, whereas the values for spleen, kidney and brain were rather similar. The greatest difference between R2 relaxation rate and R(1rho) relaxation rate at B1=10 microT was seen in the liver and muscle. The steepest slope for a dispersion curve was seen in the muscle. The value of T(1rho) approximately approached the value of T2 when the locking field B1 approached 0. Except for the liver, the calculated apparent relaxation rate R2' was slightly larger than the calculated one. The potential value of T(1rho) imaging is to combine high R1 contrast of low-field imaging with the high signal-to-noise ratio (SNR) of high static field imaging. T(1rho) relaxation and dispersion data presented in the current study help to optimize the rotating-frame MR imaging.     

Label-free imaging of arterial cells and extracellular matrix using a multimodal CARS microscope

A multimodal nonlinear optical imaging system that integrates coherent anti-Stokes Raman scattering (CARS), sum-frequency generation (SFG), and two-photon excitation fluorescence (TPEF) on the same platform was developed and applied to visualize single cells and extracellular matrix in fresh carotid arteries. CARS signals arising from CH₂-rich membranes allowed visualization of endothelial cells and smooth muscle cells of the arterial wall. Additionally, CARS microscopy allowed vibrational imaging of elastin and collagen fibrils which are also rich in CH₂ bonds. The extracellular matrix organization was further confirmed by TPEF signals arising from elastin’s autofluorescence and SFG signals arising from collagen fibrils’ non-centrosymmetric structure. Label-free imaging of significant components of arterial tissues suggests the potential application of multimodal nonlinear optical microscopy to monitor onset and progression of arterial diseases.     

Photon motion in the ECSK theory

Working within the scheme of the Einstein-Cartan-Sciama-Kibble Theory (ECSK) we find the trajectory of the photon up to its third order with respect to the velocity of slow motion sources. For the general case, discrepancies from the predictions of General Relativity (GR) are found. We apply the results to a model of polarized spin and find that in this particular case ECSK and GR theories coincide. We also perform a multipole expansion of the gravitational potentials in order to find the motion of photons far away from localized sources.     

Frequency dependence of acoustic parameters of freshly excised tissues of Sprague Dawley rats

A modified version of the pulse echo technique was used to measure the velocity of propagation and attenuation of ultrasound in excised tissue of young-adult Sprague-Dawley rats. The measurements were made at ultrasonic frequencies of 1.0, 2.25, 5.0, 7.5 and 10.0 MHz. The temperature of the tissues was monitored continuously to within ± 0.1°C of the ambient temperature. The acoustic parameters were measured in the liver, kidney, cardiac muscle and gastrocnemius muscle. All measurements were carried out in the near field region of the ultrasonic beam. It was observed that the velocity of propagation in tissues showed a slight dispersion with frequency. The attenuation in tissues increased with increasing frequency.