培氟沙星胶束包合物的荧光特性及应用

提出一种基于胶束增敏测定培氟沙星的荧光分光光度法 ,研究了培氟沙星与十二烷基硫酸钠 (SDS)形成胶束包合物的荧光性质 ,对影响荧光的不同变量和参数进行了研究和优选。试验结果表明 ,在pH 5的BrittonRobinson(BR)缓冲溶液中 ,SDS胶束对培氟沙星有强的增敏作用 ,培氟沙星胶束包合物的激发和发射波长分别为 λex =2 78nm和λem =4 32nm 。其线性范围为 0 0 6～ 1 2 0 μg·mL- 1 ,方法检出限为 0 0 6 μg·mL- 1 ,回收率为 98 5 %～ 10 0 8% ,相对标准偏差为 1 4 %～ 2 3%。该方法已成功用于片剂和血浆中培氟沙星含量的测定 ,其结果令人满意。本文采用同步导数荧光光谱法测定了血浆中的培氟沙星 ,其选择性好 ,灵敏度高 ,可有效地消除血浆中荧光背景的干扰 ,降低了检测限。     

ICP-AES法测定水中微量磷

用ICP－AES法测定水中磷，并确定了最佳测定条件，检出限为0．02μg.mL^-1，回收率为96．5％－102．1％，RSD为1．65％－2．83％。该法准确、快速、简便，应用于水中磷的测定，结果令人满意。     

胶束增敏荧光光度法测定螺内酯的研究

本文用荧光光度法对硫酸介质中螺内酯和十二烷基硫酸钠（ＳＤＳ）形成的胶束配合物的性能进行了系统研究。利用胶束的增敏增稳作用,提出了测定螺内酯的荧光光度新方法,方法线性范围宽,为１．４×１０－９－４．３×１０－６ｍｏｌ·Ｌ－１,灵敏度高,检出限为１．２×１０－９ｍｏｌ·Ｌ－１。应用本法于片剂中螺内酯的测定,结果满意     

荧光层析法研究由胶束溶液分离出的两种抗菌素

喹诺酮药物是一类重要的化学合成抗菌素 ,其临床应用正在不断扩大[1] 。氧氟沙星 (OFLX)和环丙沙星 (CPLX)结构非常相似。本文采用胶束薄层色谱法分离了两种药物。加入 1%氨水的 0 0 0 15mol·L-1十六烷基三甲基溴化铵 (CTAB)和 1%十二烷基磺酸钠 (SDS)的等体积水溶液作为展开剂 ,聚酰胺薄膜作为固定相。考查了CTAB浓度 ,SDS浓度及氨水对氧氟沙星和环丙沙星Rf 值的影响。在选定条件下 ,Rf 值分别为 0 87和 0 76 ,在激发波长为 2 80nm处对其进行了荧光测定。测定了该方法的稳定性和灵敏度。本文首次使用胶束溶液分离了氧氟沙星和环丙沙星     

胶束增效荧光光度法测定螺内酯的研究

本文用荧光光度法对硫酸介质中螺内酯和十二烷基硫酸钠（ＳＤＳ）形成的胶束配合物的进行了系统研究。利用胶束的增敏增稳作用,提出了测定螺内酯的荧光光度新方法,方法线性范围宽,为１．４×１０＾－９－４．３×１０＾－６ｍｏｌ·Ｌ＾－１,灵敏度高,检出限为１．２×１０＾－９ｍｏｌ·Ｌ＾－１,应用本法于片剂中螺内酯的测定,结果满意。     

微波消解ICP-AES法同时测定花岗石中铜、镉、铬和砷

本文采用微波消解和ICP－AES法，同时测定花岗石样品的铅、镉、铬、砷4有害元素，检出限分别为0．0008、0．0007、0．0018、0．0012μg.mL^-1,回收率为93．4％－102．5％，RSD为1．3％－3．6％，该法准确、快速、简便，结果令人满意。     

铟(Ⅲ)-8-羟基喹啉-核酸三元荧光体系的研究与应用

基于核酸对铟(Ⅲ）-8-羟基喹啉配合物的荧光增强作用，应用铟(Ⅲ)-8-羟基喹啉为荧光探针，研究了铟(Ⅲ)-8-羟基喹啉与核酸的作用，建立了新的核酸测定方法。在最佳条件下，ctDNA、hsDNA、smDNA和yRNA的线性范围分别为0．20-1．40μg／mL、0．20-1．60μg／mL、0．10—1．40μg／mL、0．20—1．20μg／mL。检出限(3o／K)分别为0．004μg／mL，0．002μg／mL，0．002μg／mL，0．002μg／mL；测定实际样品，回收率为90．9％-103．5％。     

儿童膨化食品中痕量铝的胶束增敏荧光光度法测定

本文采用了铝－8－羟基喹啉－5－磺酸（H2QS）－十六烷基三甲基溴化铵（CTMAB）胶束增敏体系，用荧光光度法对7种儿童膨化食品中痕量铝进行了测定，同时对样品的测定条件和消化处理进行了较详细的研究。方法简单、快速、灵敏度高，测定结果令人满意。     

胶束增敏荧光光度法测定螺内酯的研究①

本文用荧光光度法对硫酸介质中螺内酯和十二烷基硫酸钠(SDS)形成的胶束配合物的性能进行了系统研究。利用胶束的增敏增稳作用，提出了测定螺内酯的荧光光度新方法，方法线性范围宽，为1.4×10     

荧光共振能量转移增敏法测定锌

以巯基乙酸为稳定剂合成了CdTe量子点,并利用其与牛血清白蛋白(BSA)组成了荧光共振能量转移体系,考察BSA(供体)和CdTe量子点(受体)之间的荧光共振能量转移的最佳条件,建立了荧光共振能量转移增敏法测定Zn2+的新方法.在pH 7.4的Tris-HCl缓冲溶液中,Zn2+能对能量转移体系中CdTe量子点增敏从而测定锌的含量.Zn2+浓度在0.49-4.88μg/mL浓度范围内与CdTe量子点荧光强度呈现良好线性关系(r=0.9996),检出限为0.13μg/mL,RSD为3.2％,平均回收率为99.5％(n=5).方法适用于果汁饮料中微量锌的测定.     

Guided-Wave Two-Dimensional Acousto-Optic Scanner Using Proton-Exchanged Lithium Niobate Waveguide

Two-dimensional scanning of a 0.6328 mum guided-light beam has been realized using noncolinear acousto-optic (AO) coplanar Bragg diffraction together with colinear AO guided-mode to substrate radiation-mode conversion in a Z-cut Xpropagation LiNbO3 proton-exchanged (PE) waveguide. The two surface acoustic (SAW) waves utilized are at the center frequencies of 500 and 200 MHz, propagating in the Y and X axes, respectively. Two-dimensional scanning of approximately 720 resolvable light beam spots, namely, 18 40 (horizontal vertical) scanning, has been demonstrated using a light beam of 1.0 mm aperture. The total number of resolvable beam spots can be greatly increased from 720 by simply utilizing SAW transducers of larger bandwidth and a light beam of greater aperture. It should also be possible to significantly increase the diffraction efficiency from 3 % by optimizing the parameters of the PE waveguide and the SAWs.     

Hybrid Wavelength and Code Division Multiple Access in Optical Networks

A review of schemes for multiple access in fiber optic networks shows that a hybrid of wavelength and code division multiple access (WCDMA) combines the best features of both. In particular, the hybrid scheme retains the large information carrying capacity of wavelength division multiple access (WDMA) and flexibility of code division multiple access (CDMA). In this paper WDMA, optical CDMA (OCDMA), and WCDMA networks are discussed. In OCDMA networks, concept of incoherent and coherent coding including inverse decoding and matched filter is introduced. The delay performance of both WDMA and WCDMA networks, under the simple suboptimum access protocols based on cyclic search, is computed. It has been shown quantitatively that tuning delay significantly affects the delay performance of both WDMA and WCDMA networks. Futhermore, delay performance of WCDMA networks is always better than the WDMA networks for the same tuning delay, load, and number of users.     

Femtosecond Transform-Limited Pulse Generation by Compensating for the Linear Chirp of SPM Spectra in Dispersion Shifted Fibers

A simple technique of pulse compression, based on the linear chirp compensation of self-phase modulation (SPM) spectra in dispersion shifted fibers, is demonstrated. The optimization procedure is carried out, for a short span of a single-mode fiber, using a parabolic law, which describes the behavior of the squared output pulse width versus the pump peak power in the case of Gaussian pulses. The experimental results give a minimum pulse duration of 233 fs, which is in good agreement with the model. Shorter and coherent pulses, down to 90 fs, have been obtained by inserting an interference filter at the optical output.     

Effect ofγ-radiation on the spectral luminescence characteristics of impure cadmium tungstate crystals

Results of a study of the effect of γ-radiation on the spectral luminescence properties of cadmium tungstate crystals doped with silver, bismuth, and molybdenum cations are presented. Spectral characteristics of the nondnnnoped crystals are briefly described. Absorption and photo and X-ray luminescence spectra of the crystals taken before and after exposure to γ-radiation (5.5·10⁴ Gy) are compared. It is found that the spectral characteristics of the crystals doped with silver, bismuth, and molybdenum cations do not change markedly after the exposure. The relation between the type of impurity-induced defects, individual characteristics of the impurity cations, and the character of the effect of γ-radiation on the spectral luminescence properties of impure crystals is analyzed (preliminarily). Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 64, No. 1, pp. 55–60, January–February, 1997.     

Theoretical analysis and simulation of conjugate heights for dual-conjugate AO system in lidar

A multi-conjugate adaptive optics （MCAO） can offer a possibility of widening field of view （FOV） characterized by the isoplanatic angle, and the choose of conjugate height becomes a basic problem for MCAO, which influences the size of iosplanatic angle. Considering the application of lidar, the isoplanatic angle＇s expressions of two deformable mirrors （DMs） MCAO for uplink and downlink are deduced. The effects of conjugate heights for dual-conjugate AO are thoughtfully discussed, and the isoplanatic angles are further analyzed. The results show that the isopanatic angle varies with the conjugate height and reaches the maximum as the conjugate height is at the optimal altitude. Moreover, the optimal conjugate height changes with the propagation distance.     

Quick single-photon detector with many avalanche photo diodes working on the time division

Due to the limit of response speed of the present single-photon detector, the code rate is still too low to come into practical use for the present quantum key distribution (QKD) system.A new idea is put up to design a quick single-photon detector.This quick single-photon detector is composed of a multi-port optic-fiber splitter and many avalanche photo diodes (APDs).Au of the ports with APDs work on the time division and cooperate with a logic discriminating and deciding unit driven by the clock signal.The operation frequency lies on the number N of ports, and can reach N times of the conventional single-photon detector.The single-photon prompt detection can come true for high repetition-rate pulses.The applying of this detector will largely raise the code rate of the QKD, and boost the commercial use.     

Research on beamforming method for dual-line array with space mismatch between the two line arrays

The performance of port/starboard discrimination of dual-line array will be seriously degraded when the space between the two line arrays of the dual-line array is mismatched. The directivity function of the delay-sum and delay-subtract beamforming is deduced and the port/starboard discrimination of the delay-sum and delay-subtract beamforming is also studied both under the ideal status and the status under which the space between the two line arrays of the dual-line array is mismatched. Analysis results show the delay-subtract beamforming has better performance of port/starboard discrimination. At last, the sea trial results demonstrate the theoretical results.     

Sorting of bending magnets for the SSRF booster

The Shanghai Synchrotron Radiation Facility(SSRF)booster ring,a full energy injector for the storage ring,is deigned to accelerate the electron beam energy from 150MeV to 3.5GeV that demands high extraction efficiency at the extraction energy with low beam loss rate when electrons are ramping.Closed orbit distortion(COD)caused by bending magnet field uniformity errors which affects the machine performance harmfully could be effectively reduced by bending magnet location sorting.Considering the affections of random errors in measurement,both ideal sorting and realistic sorting are studied based on measured bending magnet field uniformity errors and one reasonable combination of bending magnets which can reduce the horizontal COD by a factor of 5is given as the final installation sequence of the booster bending magnets in this paper.