荧光层析法研究由胶束溶液分离出的两种抗菌素

喹诺酮药物是一类重要的化学合成抗菌素 ,其临床应用正在不断扩大1] 。氧氟沙星 (OFLX)和环丙沙星 (CPLX)结构非常相似。本文采用胶束薄层色谱法分离了两种药物。加入 1%氨水的 0 0 0 15mol·L-1十六烷基三甲基溴化铵 (CTAB)和 1%十二烷基磺酸钠 (SDS)的等体积水溶液作为展开剂 ,聚酰胺薄膜作为固定相。考查了CTAB浓度 ,SDS浓度及氨水对氧氟沙星和环丙沙星Rf 值的影响。在选定条件下 ,Rf 值分别为 0 87和 0 76 ,在激发波长为 2 80nm处对其进行了荧光测定。测定了该方法的稳定性和灵敏度。本文首次使用胶束溶液分离了氧氟沙星和环丙沙星

Study on Two Kinds of Antibiotics by the Fluorescence Thin-layer Chromatograph

Carbostyril medicine is a kind of important chemically synthesized antibiotics, used extensively in the clinic. Ofloxacin (OFXL) and ciprofloxacin (CPLX), being similar in structures, were separated by micellar thin layer chromatography. The 0.0015 mol.L-1 Cetyl trimethyl ammonium bromide (CTAB) and 1% sodium dodecyl sulfate (SDS) (1:1) mixture containing ammonia liquor (1%) and polyamide plates were selected, respectively, as mobile and stationary phases. Various factors affecting the Rf values of drugs on the plates such as the concentration of CTAB, SDS and ammonia liquor, were investigated. Under the appropriate condition, the corresponding Rf is 0.87 and 0.76. The fluorescence determination was carried out at the 280 nm excitation wave-length. Moreover, the stability and the sensibility were measured. The first analytical application of micellar as a mobile phase for the separation of OFLX and CPLX was developed.