Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo

This Letter reports the use of an ultrahigh resolution optical microangiography (OMAG) system for simultaneous 3D imaging of microstructure and lymphatic and blood vessels without the use of an exogenous contrast agent. An automatic algorithm is developed to segment the lymphatic vessels from the microstructural images based on the fact that the lymph fluid is optically transparent. An OMAG system is developed that utilizes a broadband supercontinuum light source, providing an axial resolution of 2.3 μm and lateral resolution of 5.8 μm, capable of resolving the capillary vasculature and lymphatic vessels innervating microcirculatory tissue beds. Experimental demonstration is performed by showing detailed 3D lymphatic and blood vessel maps, coupled with morphology, within mouse ears in vivo.     

Full range complex ultrahigh sensitive optical microangiography

This Letter presents a useful method that combines the full range complex Fourier domain optical coherence tomography (OCT) with the ultrahigh sensitive optical microangiography (OMAG) to achieve full range complex imaging of blood flow within microcirculatory tissue beds in vivo. We propose to use the fast scanning axis to realize the full range complex imaging, while using the slow axis to achieve OMAG imaging of blood flow. We demonstrate the proposed method by using a high speed 1310?nm OCT/OMAG system running at 92?kHz line scan rate to image the flow phantoms in vitro, and the blood flows in tissue beds in vivo.     

High-speed, high-resolution Fourier-domain optical coherence tomography system for retinal imaging in the 1060 nm wavelength region

A high-speed (47,000 A-scans/s), ultrahigh axial resolution Fourier domain optical coherence tomography (OCT) system for retinal imaging at approximately 1060 nm, based on a 1024 pixel linear array, 47 kHz readout rate InGaAs camera is presented. When interfaced with a custom superluminescent diode (lambda(c) = 1020 nm, Deltalambda = 108 nm, Pout = 9 mW), the system provides 3.3 microm axial OCT resolution at the surface of biological tissue, approximately 4.5 microm in vivo in rat retina, approximately 5.7 microm in vivo in human retina, and 110 dB sensitivity for 870 microW incident power and 21 mus integration time. Retinal tomograms acquired in vivo from a human volunteer and a rat animal model show clear visualization of all intraretinal layer and increased penetration into the choroid.     

超高分辨光学相干层析成像技术与材料检测应用

本文报道了一种超高分辨率谱域光学相干层析成像(SD-OCT)系统. 该系统基于超连续谱激光光源并截取部分光谱作为宽带光源, 其中心波长为665 nm, 光谱半高全宽(FWHM) 230 nm. 系统轴向分辨率0.9 μm, 轴向扫描速率28600行/秒, 横向分辨率3.9 μm, 横向视场1 mm, 最大成像深度0.6 mm(空气中). 利用研制的超高分辨率SD-OCT系统, 对不同型号的工业砂纸精细结构进行了成像, 并与普通SD-OCT的成像结果进行对比, 充分展示了研制系统在材料无损检测中优势.     

Field-enhanced scanning optical microscope

We present experimental results of an imaging technique that uses as a local probe the optical field enhanced at the junction of a scanning tunneling microscope illuminated by a p-polarized laser beam. Images of highly oriented pyrolithic graphite, recorded at a constant height mode, show a lateral optical resolution of as much as 10 nm. Approach curves exhibit sensitivity on a subnanometer scale of the optical signal to the tip-sample distance, yielding the ultrahigh vertical resolution reached in the images.     

基于重采样技术的调频连续波激光绝对测距高精度及快速测量方法研究

调频连续波激光测距方法可以实现高精度的大尺寸绝对距离测量, 且测量过程无需合作目标, 在大空间坐标精密测量领域有很高的研究价值. 而如何提高测量分辨率和实用化一直是近年来调频连续波激光绝对测距研究的热点. 本文研究了调频连续波激光测距的原理, 基于双光路调频连续波激光测距系统, 提出了通过信号拼接提高测量分辨率的信号处理优化方案, 该方案可以提高测距分辨率, 且可以降低对激光器的性能要求; 提出了可实现高速测量的简易测量方法. 设计加工了双光路光纤调频连续波激光测距系统, 利用该系统进行了测距分辨率及测距误差标定实验, 实验结果表明: 优化方案可以有效地提高测量分辨率和测量效率, 在26 m测量范围内, 测距分辨率达到了50 μm, 测距误差不超过100 μm; 快速测量方案有较高实用价值.     

双光栅快速扫描光学延迟线的色散补偿

光学相干层析成像(OCT)系统的纵向分辨力不仅与光源的带宽有关,而且与系统中两干涉臂间的色散匹配有关。如果色散没有得到精确匹配,将使光学相干层析成像系统的纵向分辨力达不到所预期的理论值。色散问题在超高分辨光学相干层析成像系统中尤为突出。提出了一种基于双光栅快速扫描光学延迟线(RSOD),用于光学相干层析成像系统的色散补偿。该方法中新增的光栅引入了光栅间距这一独立变量,其与常规单光栅快速扫描光学延迟线机构中的光栅离焦量一起,可使光学相干层析成像系统中的群速度色散(GVD)和三阶色散(TOD)同时得到补偿。分析了双光栅快速扫描光学延迟线的色散特性和色散调节原则,并提供了一个典型光学相干层析成像系统中的色散补偿实例。     

Spectral shaping to improve the point spread function in optical coherence tomography

We demonstrate inhibition of the sidelobes of the axial point spread function in optical coherence tomography by shaping the power spectrum of the light source with a remaining power of 4.54 mW. A broadband amplified spontaneous emission source radiating at 1565 +/- 40 nm is employed in a free-space optical coherence tomography system. The axial point spread functions before and after optical spectral shaping are presented. Results show that spectral shaping of the source can inhibit sidelobes of the point spread function up to 12.9 dB, with an associated small increase of 2.2 dB in noise floor in the far field. The effect of spectral shaping on axial resolution is demonstrated according to three metrics. Image quality improvement is also illustrated with optical coherence tomography images of an onion before and after spectral shaping.     

紫外像增强器分辨力校准装置与方法研究

紫外像增强器作为导弹紫外告警系统、紫外预警系统以及各类紫外辐射监测系统的核心部件，其参数准确与否，直接影响到系统的图像质量。为保证测试数据的准确性，研制紫外像增强器分辨力校准装置，校准装置所用紫外光源是波长范围为200 nm～400 nm的紫外光，相对应的分辨力靶、滤光处、光学成像系统均要求能够透射紫外光，由于紫外波长较短，容易引起散射效应而产生大量的杂散光，设计的分辨力靶采用紫外级石英，紫外光学成像系统采用透射式结构，选用同轴共轭透镜作为紫外光学成像系统。实验和测量不确定度分析验证校准装置的测量不确定度为5%。     

Experimental study on the imaging of the squeezed state light at 1064 nm

Stable, low noise, infrared squeezed state tight at 1064 nm is generated by utilizing optical parametric down-conversion (OPDC) technique based on periodically poled KTiOPO₄ in a optical parametric amplifier (OPA) resonator. A non-classical noise reduction of 2.58 dB below the shot noise is observed through balanced homodyne detection. The squeezed state light is used to set up an imaging system for high-resolution imaging, and it is found that the resolution of image based on the amplitude-squeezed light is 1.26 times larger than that of infrared coherent light under the same intensity. The experimental results indicate that squeezed light is an important non-classical light, which can overcome the coherent laser shot-noise, the classical diffraction limit and limit of quantum noise.     

利用变迹术和相干门相结合实现光学相干层析成像术轴向超分辨

光学相十层析成像（光学相干层析成像术）的轴向分辨力由光源带宽和探测光束的聚焦条件共同决定。提高光学相干层析成像术轴向分辨力的方法主要基于带宽光源技术。提出了一种将变迹术与光学相十层析成像术相干门有机结合的方法来提高其轴向分辨力。通过适当形式的光瞳滤波器．使光学相干层析成像术系统轴向响应的主瓣宽度缩小到相干门之内,而其旁瓣则处于相干门之外．不对相干成像产生有效贡献。这样．就能在光源带宽不变的条件下,有效提高光学相十层析成像术的轴向分辨力,避免了采用宽带光源所带来的费用昂贵和系统复杂等缺陷。     

A combined multiple-SLED broadband light source at 1300 nm for high resolution optical coherence tomography

We demonstrate a compact, inexpensive, and reliable fiber-coupled light source with broad bandwidth and sufficient power at 1300 nm for high resolution optical coherence tomography (OCT) imaging in real-time applications. By combining four superluminescent diodes (SLEDs) with different central wavelengths, the light source has a bandwidth of 145 nm centered at 1325 nm with over 10 mW of power. OCT images of an excised stage 30 embryonic chick heart acquired with our combined SLED light source (<5 μm axial resolution in tissue) are compared with images obtained with a single SLED source (∼10 μm axial resolution in tissue). The high resolution OCT system with the combined SLED light source provides better image quality (smaller speckle noise) and a greater ability to observe fine structures in the embryonic heart.     

投影式头戴静脉显像光学系统的设计

为了辅助静脉穿刺及相关的医疗操作, 设计了一款基于投影式头戴显示器(HMPD)的静脉显像系统, 其光学系统由近红外成像系统和穿透型HMPD构成。利用光学设计软件ZEMAX优化设计近红外成像系统, 使其具有F/2.6的大数值孔径, 有利于弱反射红外光的收集成像。穿透型HMPD采用与近红外成像系统相同的光学结构, 有利于简化系统的加工装调。设计结果表明, 近红外成像系统成像质量优异, 分辨率达到QXGA(2 048×1 536)。穿透型HMPD具有18 mm的大出瞳直径及25 mm的大出瞳距离, 场曲小于0.03 D, 畸变小于0.32%, 达到QXGA分辨率显示模式。与现行的静脉显像系统相比, 本显像系统结构简单紧凑、佩戴舒适, 且具有超高分辨率, 是一款适用于辅助医疗的目视系统。     

Passive ranging and three-dimensional imaging through chiral phase coding

We present a method for single-image passive ranging and three-dimensional (3D) imaging in incoherent light based on chiral phase coding. A chiral linear phase variation across the aperture of an optical system results in a frequency response with a characteristic pattern of fringes such that the spatial period and inclination of the pattern depend on the focusing error. From this dependency, the absolute focusing error and, hence, the distance to the object can be found. In the experiments a resolution of ~1.4 μm is achieved with a 20 mm aperture lens in a 4 mm interval at a distance of 140 mm from the lens. A resolution of ~0.7 mm is obtained at a distance of ~11 m with the range finder employing two 25.4 mm spherical mirrors spaced apart by ~140 mm. We also demonstrate 3D imaging of weakly textured objects.     

宽光谱超高速八分幅相机的光学系统设计

为满足数字超高速成像需求,提高相机的时间分辨率,设计了一种工作于350 nm~800 nm宽波段的八分幅相机光学系统。该系统采用光阑外置的会聚光分光结构,可同时获得同一物面的八幅相同图像,针对光学系统后截距、像方数值孔径等重要光学参数对像面照度差的影响、宽光谱成像的色差校正等问题进行了分析。在Code V中对中继镜头进行设计优化,像面大小可达26 mm,像方MTF在40 lp/mm时对比度达0.5以上,畸变小于1%,分幅后系统像面照度差在±10%以内。模拟结果表明:八分幅相机光学系统各幅图像一致性较高。     

超短脉冲激光瞄准装置光学系统设计

为了提高超短脉冲激光的瞄准精度,基于自准直原理提出瞄准装置光学系统。以670 nm光纤耦合激光器为光源,设计指示光准直、扩束光学系统,准直光的不平行度达到3.2,设计焦距为350 mm,相对孔径1/5,离轴量50 mm的主激光离轴抛物面镜,其成像质量达到衍射极限,基于准直束光学系统和离轴抛物面镜,设计可适应670 nm和800 nm两种波长的20和100的瞄准和监测成像光学系统。提出一种小孔准直的安装调试方法,以指示光进行实验验证,结果表明:设计的光学系统成像光斑均匀,其物方分辨率达到4.1 m。     

Ultrahigh-resolution optical coherence tomography using continuum generation in an air-silica microstructure optical fiber

We demonstrate ultrahigh-resolution optical coherence tomography (OCT) using continuum generation in an air-silica microstructure fiber as a low-coherence light source. A broadband OCT system was developed and imaging was performed with a bandwidth of 370 nm at a 1.3-mu;m center wavelength. Longitudinal resolutions of 2.5 microm in air and ~2 microm in tissue were achieved. Ultrahigh-resolution imaging in biological tissue in vivo was demonstrated.     

Imaging of subcutaneous blood vessels and flow velocity profiles by optical coherence tomography

We have applied a compact low power rapid scanning Doppler Optical Coherence Tomography system to monitor multi-dimensional velocity profiles within the complex vessels and simultaneous real-time non-invasive imaging of skin tissues morphology in vivo, in the wavelength range of 1.3–1.5 nm. Optical clearing of skin tissues has been utilized to achieve depth of OCT images up to 1.7 mm. Current approach enables applying low-power (0.4–0.5 mW) and low-noise broadband near-infrared light sources and obtaining OCT images with down to 12 μm spatial resolution. Two-dimensional time-domain OCT images of complex flow velocity profiles in blood vessel phantom and in vivo subcutaneous human skin tissues are presented. The effect of optical clearing on in vivo images is demonstrated and discussed.     

基于离轴两反利特罗结构的棱镜高光谱成像系统的光学设计

为满足高光谱成像系统高空间分辨率和高光谱分辨率的要求,并应对实际应用中对仪器小型化、轻量化、高光学效率的新需求,研究一种基于利特罗结构的棱镜色散高光谱成像系统,采用离轴两反的利特罗结构形式减小光学系统的体积,同时为平面棱镜提供准直光路,并以宏编程的优化方式,避免系统中光路干涉。结果表明,通过非球面反射镜和双校正透镜的设计,该光学成像系统的谱线弯曲均小于2.1 μm,色畸变小于1.3 μm,控制在18%像元内,在400～1 080 nm可见—近红外(VNIR)工作波段的光学调制传递函数(MTF)均达到0.9以上,光谱分辨率为1.6～5.0 nm,光谱透过率在51.5%以上,系统在整个工作光谱范围都具有较高的透过率和像质。     

基于DMD光谱可调的星模拟器光源光学系统设计

为满足在各种谱线分布下对星敏感器探测能力的高准确度标定,提出了一种基于数字微镜器件的光谱可调星模拟器光源光学系统设计方法,以解决星模拟器与星敏感器观星色温不匹配对星敏感器光信号定标准确度产生的问题.首先,根据设计指标选取Czerny-Turner型光学系统为光源光学系统,对Czerny-Turner型光学系统的彗差和象散进行分析,选取消彗差的Czerny-Turner结构;其次利用MATLAB程序求解Czerny-Turner型光学系统初始结构并应用ZEMAX对其进行优化;最后对光学系统进行公差分析.公差分析结果表明,在400~1 100nm的工作谱段范围内,光学系统的光谱分辨率小于2nm,设计结果满足要求,有效降低了光谱不匹配带来的定标误差.