强恒磁场对动物血清中锌铜影响的研究

用ＡＡＳ研究了加磁前后的家兔和小鼠血清中锌，铜含量的变化。结果表明，家兔加磁１６天后血清中锌较加磁前无显著差异（Ｐ＞０．０５），而血清中铜则显著降低（Ｐ＜０．０５）；小鼠加磁１６天，血清中锌，铜较对照组均有显著的降低（Ｐ＜０．０１）。     

火焰原子吸收法测定60名肝炎患者血清中的铜

用火焰原子吸收法测定６０名肝炎患者和３０名正常对对照组成员血清中的铜，结果表明，肝炎患者的血清铜值明显升高，与正常对照比较有极有显著差异（Ｐ〈０．０１）。     

石墨炉原子吸收法测定人体血清铜

本文叙述了一种用石墨炉原子吸收法测定微量血清铜的方法。血清经硝酸溶液稀释10倍后，可直接测定。操作简便，快速，特别是取样量少，仅需取血20微升（约一滴）。回收率为96－109，本方法的绝对灵敏度是3.5×10^-11克，血清铜含量为1.4毫克／升时，相对标准偏差为1.13%。     

原子吸收光谱法检测冠心病,糖尿病及高脂血症患者血清中微…

本文应用原子吸收光谱法检测了冠心病，糖尿病及高脂血病人血清中微量元素－铜、锌和常量元素－钙，镁。结果表明：铜，锌的代谢异常可能是冠心病，高脂血症患者发病的因素之一，而糖尿病人存在着不同程度的锌缺乏症。     

原子吸收光谱法检测冠心病、糖尿病及高脂血症患者血清中微量元素

本文应用原子吸收光谱法检测了冠心病、糖尿病及高脂血病人血清中微量元素—铜、锌和常量元素—钙、镁。结果表明：铜，锌的代谢异常可能是冠心病，高脂血症患者发病的因素之一，而糖尿病人存在着不同程度的锌缺乏症。     

GFAAS测定乙醇-EDTA法分离血清中不同形态的铜和锌

血清中元素不同形态含量变化与人体营养和健康有着密切关系。文章利用两步沉淀蛋白质-石墨炉原子吸收法测定了血清中非蛋白结合的铜和锌。无水乙醇以2∶1与血清混合后,再经70 ℃温度变性处理,通过贝克曼离心机高速离心,可将蛋白质完全沉降。在血清中加入EDTA可将白蛋白结合的铜和锌脱落,转化为非蛋白结合铜和锌,从而可测得球蛋白和白蛋白分别结合的铜和锌含量。用氘灯校正背景,石墨炉原子吸收法进行测定,方法检测限：Cu,1.2 μg·L-1; Zn,0.098 μg·L-1。回收率：Cu,92.3%～104%,Zn,90%～107%。利用此方法测定了健康人及肿瘤患者血清和肝豆状核性患者中球蛋白、白蛋白及结合的铜与锌以及游离的铜和锌,并与健康人作了对比。     

ICP—AES法测定尿毒症病人血清中7种微量元素

本法测定了尿毒症病人血清中锌、钙、铁、铜、镁、铬及镉等７种微量元素的含量。本方法具有灵敏度高，操作简单，基体干扰少等优点。     

芬布芬-铜（Ⅱ）-牛血清白蛋白相互作用的光谱法研究

模拟生理条件下,应用荧光光谱法研究了芬布芬对牛血清白蛋白,铜（Ⅱ）对牛血清白蛋白以及铜（Ⅱ）对芬布芬和牛血清白蛋白荧光光谱特性的影响.实验表明：铜（Ⅱ）和芬布芬均可使牛血清白蛋白的荧光强度发生静态猝灭,并且在铜（Ⅱ）存在下,芬布芬对牛血清白蛋白的荧光猝灭作用显著增强.根据荧光猝灭双倒数图计算芬布芬和牛血清白蛋白的结合常数为8.44×104,结合位点数为0.97.荧光猝灭双倒数图计算的结果表明,芬布芬和牛血清白蛋白之间的结合常数和结合位点数均随铜（Ⅱ）浓度的增大而增大.很据三者结合反应的研究,进一步探讨了芬布芬、铜（Ⅱ）在生物体内与蛋白质相互作用的机理.     

肝豆状核变性病人血清和尿中铜的测定及其临床意义

本文为拓宽分析仪器在临床医学中的应用 ,建立了肝豆状核变性病人血清和尿中铜的火焰原子吸收光谱测定方法。方法的相对标准偏差小于 1.4 % ,检出限为 7 3× 10 - 3μg·mL- 1 ,加标回收率在 94 %～10 4 %之间。讨论了肝豆状核变性病人血铜和尿铜水平对临床诊断及治疗的意义。实验结果表明 ,血清铜和尿铜水平对于WD虽不具单独诊断意义 ,必须结合临床症状和其他生化指标综合诊断 ,但它们却是诊断本病的重要参考指标 ,尤其在治疗方面 ,及时监控血铜水平和尿铜排出量具有指导意义。本法已广泛应用于临床实际样品的分析 ,获得良好效果。     

ICP－AES法测定尿毒症病人血清中7种微量元素

本法测定了尿毒症病人血清中锌、钙、铁、铜、镁、铬及镉等７种微量元素的含量。本方法具有灵敏度高，操作简单、基体干扰少等优点。     

Spectroscopic Studies on the Interaction Between Aucubin and Bovine Serum Albumin Without or With Copper II or Iron III

The binding of aucubin to bovine serum albumin in the absence or presence of copper II or iron III has been studied by fluorescence, UV-Vis absorbance, synchronous fluorescence, and circular dichroism spectroscopies at pH 7.40. The results of fluorescence showed that the static quenching mechanism played a major role without or with copper II or iron III, and the quenching constant, binding constant, and binding site number decreased with copper II or iron III at three different temperatures (310 K, 300 K, and 290 K). This indicated that the drug would take effect more promptly in the presence of metal ions than in the absence of them. Thermodynamic parameters revealed that hydrophobic forces played vital roles and the binding process was spontaneous without or with copper II or iron III. The results of synchronous fluorescence showed that the polarity of the microenvironment around tryptophan and tyrosine residues changed insignificantly without or with copper II or iron III. The results of circular dichroism showed that there were slight reductions in the α-helix content of bovine serum albumin. In conclusion, copper II or iron III could reduce the binding ability between aucubin and bovine serum albumin, resulting in enhanced maximum effects of aucubin. The relative knowledge would contribute to the pharmaceutical development and clinical application of aucubin.

Supplemental materials are available for this article. Go to the publisher's online edition of Spectroscopy Letters to view the supplemental file.     

Colouration mechanism of underglaze copper‐red decoration porcelain (AD 13th–14th century), China

Underglaze copper‐red decoration, i.e. the copper colourant used to paint diversified patterns on the surface of a body and then covered by transparent glaze and fired at high temperature in a reductive firing environment, is famous all over the world. However, the red colouration mechanism generated by underglaze copper remains unclear. In particular, the fact that the edges of the red patterns are orange has been ignored in previous research. Here, non‐destructive analysis has been carried out on a precious fragment of early underglaze red porcelain using synchrotron radiation X‐ray fluorescence, X‐ray absorption near‐edge spectroscopy (XANES) and reflection spectrometry techniques. The results suggest that the copper content in the red region is higher than that in the orange region, and other colour generation elements do not have obvious content difference, indicating that the colour generation effect of the underglaze red product is related to the copper content. XANES analysis shows that the valence states of copper in the red and orange regions are similar and metal copper contributes to their hues. The results of reflection spectrometry demonstrate that tiny orange hues could be attributed to the Mie scatting effect. Therefore, light‐scattering effects should be considered when researching the colouration mechanism of underglaze red.     

Synthesis,characterization, and thermo-mechanical properties of copper-loaded apatitic calcium phosphates

Copper is well known as a classical transition metal used in heterogeneous catalysis. In this study, copper-loaded apatitic calcium phosphates were prepared using incipient wetness impregnation (IWI) and ionic exchange (IE) methods. The interaction between copper precursor (copper nitrate trihydrate, Cu(NO₃)₂?3H₂O) and apatitic calcium phosphate (CaP) depended strongly on the preparation method and the content of copper-loaded. Using IE, copper(II) cations (Cu²⁺) were incorporated in the apatitic structure of CaP. The content of copper(II) cations seemed to be limited at about 2.2?wt.%. Calcination at 400?°C had no influence on the solids obtained by the IE method. Using IWI, the deposition of a theoretical copper content of 2?wt.% led to the incorporation of copper(II) cations in the apatitic structure of CaP by IE with Ca²⁺, despite the low quantity of aqueous solvent used. Therefore the resulting product was similar to that obtained by IE. When the theoretical copper content rose to 20?wt.%, the entire amount of copper precursor molecules were largely deposited, which resulted in the formation of copper oxide particles (CuO) after air calcination at 400?°C. Thermo-mechanical analysis study showed that the presence of copper oxide did not modify the thermal shrinkage of the initial calcium phosphate. On the other hand, thermal shrinkage was much more important in the case of CaP substituted with copper(II) cations.     

原子吸收光谱法测定中药厚朴中的铜、铁和钙

用硝酸-高氯酸（4∶1）混合液对厚朴进行消解,然后采用原子吸收光谱法测定厚朴中铜、铁、钙的含量.结果表明,厚朴中含铜4.41μμg/g,含铁55.5 μg/g,含钙6872.5μg/g,加标回收率在93.6％-100.1％之间.该方法测定中药中金属离子的含量方法简便、快速、灵敏.该结果可为进一步研究厚朴的相关药理作用提供参考依据.     

Structural and Mössbauer studies of aerosol FeCu nanoparticles in a wide composition range

Structure and magnetic state of aerosol FeCu nanoparticles of 10–30 nm size with Cu content of 0.6–92.1 at.% have been examined by X-ray diffraction and Mössbauer spectroscopy. The FeCu particles have been shown to consist of an iron core surrounded by a copper and Fe oxide shell. With increasing Cu content the iron core having a bcc structure is reduced down to its complete disappearance followed by vanishing ferromagnetism of the particles. Within the copper content from 4.9 to 74.3 at.% the bcc and fcc phases coexist, with the fcc phase having a lattice constant close to that of pure copper and the bcc lattice constant being slightly higher than that for pure Fe due to embedding Cu atoms into the Fe lattice. At Fe-rich FeCu samples a presence of two-spin (ferromagnetic and paramagnetic) components of the fcc Fe is also observed. In the case of a thin copper shell there is only the ferromagnetic fcc Fe, whereas with further thickening of the shell both spin states of the fcc Fe appear existing up to a 20% Cu content. For FeCu samples with a higher Cu content they disappear due to oxidation of the copper grains. The Cu-rich samples with Cu content higher 80 at.% have a fcc structure, with the lattice constant being slightly higher than that of copper and they are paramagnetic. A slight increase of the lattice constant is due to the penetration of small iron aggregations into the Cu grains. In contact with air, the FeCu particles become covered with Fe₃O₄ and Cu₂O. Their long-term exposure to ambient conditions leads to further oxidation process of Cu₂O to CuO.