肝豆状核变性病人血清和尿中铜的测定及其临床意义

本文为拓宽分析仪器在临床医学中的应用 ,建立了肝豆状核变性病人血清和尿中铜的火焰原子吸收光谱测定方法。方法的相对标准偏差小于 1.4 % ,检出限为 7 3× 10 - 3μg·mL- 1 ,加标回收率在 94 %～10 4 %之间。讨论了肝豆状核变性病人血铜和尿铜水平对临床诊断及治疗的意义。实验结果表明 ,血清铜和尿铜水平对于WD虽不具单独诊断意义 ,必须结合临床症状和其他生化指标综合诊断 ,但它们却是诊断本病的重要参考指标 ,尤其在治疗方面 ,及时监控血铜水平和尿铜排出量具有指导意义。本法已广泛应用于临床实际样品的分析 ,获得良好效果。

Determination of Copper in Serum and Urine of Hepatolenticular Degeneration Patient and Their Clinic Meaning

A method for the determination of copper in hepatolenticular degeneration patient's serum and urine by flame atomic absorption spectrometry was developed, and the meaning of clinic diagnosis and therapy for copper lever of hepatolenticular degeneration patient in serum and urine was discussed. The detection limit and linear range for copper are 7.3 x 10(-3) micrograms.mL-1 and 0.008-5 micrograms.mL-1 respectively. The relative standard deviation is less than 1.4% and the recovery is 94%-104%. The experimental results show that serum copper and urine copper contents are really important reference index for diagnosing the disease, with guiding significance for timely monitoring and controlling serum copper and urine copper excretion, especially in therapy, although they do not have separate diagnosing significance for hepatolenticular degeneration disease and have to be combined with clinic symptoms and other biochemical indexes to make a comprehensive diagnosis. This method has been applied to clinic diagnosis and therapy with satisfactory results.