电化学发光传感器在农业传感领域中的研究进展

基于电化学发光(ECL)分析法构建的电化学发光传感器具有灵敏度高、背景信号低、操作简单的优点,因此,它在农业、工业、环境、临床和食品等领域具有广泛的应用前景。 本文主要综述了电化学发光传感器检测农药残留和真菌毒素的应用及其相应的检测性能,分析了电化学发光传感器在农业传感领域的研究现状,并阐述了未来电化学发光传感器的发展趋势。     

分子印迹电化学发光分析

分子印迹电化学发光兼具分子印迹技术及电化学发光方法两者的优点,即高灵敏度、高选择性、可控性好、易于微型化和操作简单等特点。近几年来在生物仿生传感器、有害农药残留物质及食品安全监测等方面具有广泛的应用。本综述简要介绍分子印迹电化学发光传感器及分子印迹固相萃取电化学发光的概况,并对其今后的研究趋势进行展望。     

基于分子印迹技术的电化学发光分析

基于分子印迹技术的电化学发光分析是近几年刚刚发展起来的新型分析方法,兼具分子印迹技术与电化学发光方法两者的优点,具有高灵敏度、高选择性、可控性好、易于微型化和操作简单等特点,在生命科学、食品安全及环境监测等领域有着广泛的应用前景。本综述简要介绍了常用的电化学发光体系和基本原理,综述了近年来分子印迹电化学发光分析的主要研究进展,对不同类型分子印迹电化学分析的构建方法、原理及所构建方法的性能（包括灵敏度、选择性、检测范围和稳定性等）进行了评述。基于分子印迹技术的电化学发光分析主要可以分为三类：制备固态发光电极、非固态发光电极构建分子印迹电化学发光传感器和分子印迹固相萃取与电化学发光分析联用,其中制备固态发光电极用于构建分子印迹电化学发光传感器最有发展前景。最后,本综述也对分子印迹电化学发光分析今后的发展趋势和方向进行了展望。     

联吡啶钌电化学发光传感器测定海洛因

利用离子液体为粘合剂制作碳糊电极,采用高分子聚合法,合成包埋有Ru(bpy)2(dcbpy)2+的高分子聚合物,将钌聚合物掺杂于离子液体碳糊电极中,制作电化学发光传感器.结果表明,此传感器具有很好的电化学发光特性,与用石蜡油为粘合剂制作的电化学发光传感器相比,离子液体为粘合剂的电化学发光传感器检测三丙胺的检出限降低1个数量级.海洛因对电化学发光传感器的发光信号有很好的增强作用,基于此建立了高灵敏度检测海洛因的电化学发光分析法,海洛因浓度与电化学发光信号在2.0×10-9～2.0×10-5 mol/L范围内呈良好的线性关系,检出限为8×10-10 mol/L (S/N=3).将电化学发光传感器在5.0×10-9 mol/L海洛因溶液中采用线性循环电位连续扫描60圈,相对标准偏差小于2.2%.本方法用于血清中海洛因的检测,其回收率为94%～101%.     

基于电化学双共价键法构建可再生高灵敏的电化学发光适体传感器检测可卡因

基于点击化学和重氮盐法的双共价键固定化方法,制备了一种高灵敏、可重复使用的电化学发光(ECL)适体传感器. 该方法以可卡因为分析物,以可卡因适体为分子识别物质,以钌联吡啶衍生物为ECL信号物质. 采用电化学方法在玻碳电极表面重氮化叠氮苯胺,通过点击反应连接炔基功能化的钌联吡啶衍生物标记可卡因适体,获得适体传感器. 该传感器在共反应剂存在下,产生弱的电化学发光信号,可卡因存在下,电化学发光信号增加. 基于此,建立了“信号增强”型检测可卡因的电化学发光分析新方法. 电化学发光信号与可卡因浓度在0.1 nmol·L^-1 ~ 100 nmol·L^-1范围内呈良好的线性关系,检出限为60 pmol·L^-1. 该传感器具有良好的稳定性,可重复多次使用. 该双共价键法在构建ECL传感器方面具有很好的应用前景.     

硫化镉量子点-纳米金复合颗粒电化学发光传感器研究DNA的损伤

采用电化学发光方法研究了全氟辛酸(Perfluorooctanoic acid,PFOA)对DNA的损伤。结合量子点(Quantum dots,QDs)及纳米金(Nano gold,NG)颗粒的独特性能,制备了量子点-纳米金复合颗粒。将小牛胸腺DNA(ct-DNA)修饰在玻碳电极表面,然后修饰量子点-纳米金复合颗粒,构建了基于纳米金的量子点电化学发光传感器,研究了纳米金对量子点发光强度的增强作用,并利用该传感器进一步研究了PFOA对ctDNA的损伤作用。采用原子力显微镜(AFM)及X射线光电子能谱(XPS)技术对修饰电极的表面形态进行表征。实验结果表明,与单一的量子点电化学发光传感器相比,纳米金-量子点复合物电化学发光传感器的发光强度增大了近4倍。同时,ct-DNA经PFOA温浴作用后,电化学发光强度发生显著降低,表明PFOA导致了ct-DNA损伤。     

碳纳米纤维糊电极电化学发光法测定甲硫哒嗪

制备了基于碳纳米纤维糊电极(CFPE)的新型电化学发光传感器.运用电化学方法对CFPE进行了表征,并考察了三联毗啶钉和甲硫哒嗪在此电极上的电化学行为和电化学发光行为.结果表明,该电极表现出很好的电化学活性和电化学发光响应.基于甲硫哒嗪对三联吡啶钉电化学发光的增强作用,建立了测定甲硫哒嗪的电化学发光新方法.实验考察了缓冲...     

联吡啶钌电化学发光研究进展

联吡啶钌电化学发光在免疫分析、核酸分析、共反应物分析和适配子传感器等方面具有广泛的应用前景,成为在诸多电化学发光体系如9,10-二苯基蒽、光泽精、联吡啶钌、过氧化草酸酯、鲁米诺、石墨烯和量子点等之中近年来国际上研究最多的电化学发光体系之一.本综述对已发表的绝大多数联吡啶钌电化学发光成果加以归纳总结,简要介绍联吡啶钌电化学发光的概况,并尝试展望其今后的研究趋势.     

分子印迹-电化学发光技术研究进展

分子印迹-电化学发光技术具有分子印迹技术的高选择性及电化学发光技术的高灵敏性,以及发光易于调控、稳定性好、便于微型化和仪器操作简单等优点,已被广泛地应用于重金属检测、免疫传感技术、基因传感技术、酶传感技术、食品安全与药物分析等领域。该文结合本实验室的研究工作介绍了分子印迹电化学发光传感器的原理和构建思路。在此基础上,着重介绍了分子印迹电化学发光技术在食品安全与药物分析中的应用,并对其今后的研究趋势进行了展望。     

金属有机框架材料在电化学/电化学发光免疫分析中的应用（英文）

设计和研制具有超灵敏、高精度、选择性好的免疫传感器对于疾病的早期诊断和筛查以及疾病治疗过程的监测具有十分重要的意义。其中,电化学免疫分析法和电化学发光(ECL)免疫分析法,由于具有稳定性好、灵敏度高、线性范围宽、可控性好等优点而备受关注,已成为当前的研究热点之一。金属有机框架(MOFs)作为一类新型的多孔晶体材料,由于其具有比表面积大、化学稳定性好、孔径和纳米级骨架结构可调节等优点,在电化学和ECL免疫传感器的制备中得到了广泛的应用。MOFs不仅可以作为固定生物识别分子的敏感平台,还可以用于富集痕量分析物和信号分子来放大分析信号,提高电化学或ECL免疫分析的灵敏度。目前,科研人员已合成各种各样具有不同性能和形貌的MOFs纳米材料,并用于开发高性能的电化学免疫传感器和ECL免疫传感器。本文综述了不同类型的基于MOFs纳米材料的电化学/ECL免疫传感器的制备及其在免疫分析中的检测应用。研究表明,MOFs不仅可以作为电极表面修饰的基底、信号探针(包括电活性标记分子和电化学发光发光标记探针)、催化活性标记物,还可以作为负载各种生物分子、纳米材料的载体,最终可用于灵敏的电化学和ECL检测。此外,...     

Ultra Flexible Paper Based Electrochemical Sensors: Effect of Mechanical Contortion upon Electrochemical Performance

The influence of mechanical contortion upon the electrochemical performance of screen‐printed graphite paper‐based electroanalytical sensing platforms is evaluated and contrasted with traditionally employed polymeric based screen‐printed graphite sensors. Such a situation of implementation can be envisaged for the potential sensing of analytes on the skin where such sensors are based, for example in clothing where mechanical contortion, viz, bending will occur, and as such, its effect upon electrochemical sensors is of both fundamental and applied importance. The effect of mechanical contortion or stress upon electrochemical behaviour and performance is of screen printed sensors is explored. Comparisons are made between both paper‐ and polymeric‐ based sensing platforms that are evaluated towards the sensing of the well characterised electrochemical probes potassium ferrocyanide(II), hexaammine‐ruthenium(III) chloride and nicotinamide adenine dinucleotide (NADH). It is determined that the paper‐based sensors offer greater resilience in terms of electrochemical performance after mechanical stress. We gain insights into the role played by both the effect of the time of mechanical contortion and additionally the potentially detrimental effects of repeated contortion are explored. These unique paper‐based sensors hold promise for widespread applications where flexible and ultra‐low cost sensors are required such as applications into medical devices were ultra‐low cost sensors are a pre‐requisite, but also for utilisation within applications which require the implementation of ultra‐flexible electroanalytical sensing platforms such as in the case of wearable sensors, whilst maintaining useful electrochemical performances.     

Electrochemical Sensing in Contact Lenses

Electrochemical sensors embedded in hydrogel-based contact lenses provide valuable health-related information, enabling non-invasive and real-time continuous monitoring. Recently, considerable progress has been made in tear based electrochemical sensors. The scope of reported analytes is continuously expanding. This review identifies key chemical biomarkers (including metabolites, ions, proteins) that can be electrochemically detected in tears. The working principles of i) amperometric enzymatic biosensors, ii) ion-selective sensors for pH and ions, iii) voltammetric sensors and iv) affinity sensors are summarized. This review provides guidelines for the future development of contact lens based electrochemical sensors.     

High effective sensors based on photonic crystals

Photonic crystals have been extensively studied as high effective sensors for environmental monitoring and chemical and biological detections. This paper reviews recent achievements on photonic crystal sensors. Especially, the band gap responsiveness and the ability in amplifying spontaneous emission are demonstrated in the reported photonic crystal monitors/sensors. They are of great importance for optical monitors/sensors visualized by the naked eye and sensors based on fluorescence applications. The photonic crystal sensors are promising for low-cost and high effective sensors and detection methods, although challenges still exist in practical applications.     

Optical chemical sensors for the detection of explosives and associated substances

The main analytical characteristics of optical chemical sensors for detecting the vapors and microparticles of explosives and associated substances are compared. The limits of detection, sensitivity, sensor setting time (response speed) and recovery time after the action of an analyte, and the selectivity of fluorescence sensors, chemiluminescence sensors, surface-enhanced Raman sensors, surface plasmon resonance sensors, absorption integrated optical waveguide sensors, waveguide interferometric sensors, and ring resonator based sensors. The effectiveness of the use of nanosized structures and bio- and nanostructured specific coatings in optical sensors is analyzed.     

非质量效应压电化学传感的应用与发展

压电化学传感器在学术界通常称为压电石英微平天,归属于质量传感器范围,但是在液相中,这种传感器还要受到液相物理化学特性参量的影响,据此,提出与发展了区别于常规质量响应型压电传感的另一类非质量响应型压电传感检测技术,本文论述了在相应领域中的应用研究,其中不少领域,传统的微天平型压电传感器根本不适用,或者传感性能不如新型的基于非质量效应响应机理的压电传感器。     

喹啉衍生物锌离子荧光传感器*

本文综述了近年来用于锌离子检测的喹啉衍生物荧光传感器的研究进展,介绍了基于喹啉、8-羟基喹啉和8-氨基喹啉合成的锌离子荧光传感器的结构和设计原理,概述了锌离子对喹啉衍生物荧光传感器光学性能的影响,分析了喹啉衍生物锌离子荧光传感器在检测过程中的优点及其实际应用价值,并展望了这类锌离子荧光传感器的研究和发展方向。     

Molecular tubes for lipid sensing: tube conformations control analyte selectivity and fluorescent response

Two fluorescent sensors for lipids have been prepared and tested for detection of a number of hydrophobic compounds of varying shape and size. The data suggest that the two sensors have a different mode of fluorescent response. Yet, the two sensors are only different in the bridging group--one having a flexible amide and one having a rigid allyl bridge. The fluorescence data are explained based on a difference in conformation of the two sensors in aqueous solution.     

Application of microbiological sensors in fermentation processes

A review is presented of microbiological sensors which are composed of micro-organisms immobilized in a membrane and coupled to a sensing element. Conventional microbiological sensors such as those for biochemical oxygen demand (BOD), ethanol and acetic acid are discussed briefly. Novel sensors are then described. The sensor for carbon dioxide is based on a chemoautotrophic bacterium, that for alcohol on cell membranes of the acetic acid bacteria, Gluoconabacter suboxydans. Sensors for BOD carbon dioxide are based on thermophilic bacteria. Finally, a microbial field effect transistor sensor (FET) for alcohol sensor is described. For all the sensors, the ranges of linear response and their long-term stabilities are reported.     

A Multivariate Liner Model for Calculating Response Feature Parameters of Chemical Sensor Based on Fluorescence Quenching for Analytes

The chemical membrane sensors based on fluorescence quenching are the important field of the chemical sensors, it is conducive to the development of the chemical sensors to inquire into their fluorescence quenching mechanism and evaluate their response parameters.