电离辐射诱导组蛋白乙酰化引起的染色质结构解聚研究

真核生物的DNA分子经高度压缩以染色质形式存在于细胞核中,染色质动态结构在DNA复制、基因转录和DNA修复等过程中起着重要的调控作用。核内染色质结构的原位高分辨解析和其结构变化定量表征一直受困于显微成像观测分辨率的限制。通过点击化学荧光标记EdU和STORM单分子定位显微成像,实验得到了细胞核内超分辨率染色质结构图像。基于提出的单分子团簇分析和最近邻距离算法分析发现,X射线辐照和TSA处理后的细胞核内核小体团簇数量显著增多,核小体团簇所占细胞核内的面积比相对于对照组增加,且团簇内平均EdU分子数降低。同时,重离子辐照活细胞在线成像实验获得的XRCC1招募动力学速率常数表明乙酰化处理使得DNA损伤密度降低。这些结果表明电离辐射和乙酰化处理均导致了染色质结构的松散化。STORM超分辨成像方法和分析算法及其获得的核小体团簇分布规律为染色质结构的松散提供了直接的定量表征数据支持。     

Twist neutrality and the diameter of the nucleosome core particle

The diameter of the nucleosome core particle is the same for all the eukaryotes. Here we discuss the possibility that this selectiveness is consistent with a propensity for twist neutrality, in particular, for the double helical DNA to stay rotationally neutral when strained. Reorganization of DNA cannot be done without some level of temporal tensile stress, and as a consequence chiral molecules, such as helices, will twist under strain. The requirement that the nucleosome, constituting the nucleosome core particle and linker DNA, has a vanishing strain-twist coupling leads to a requirement for the amount of bending. For the diameter of the coiled DNA we obtain the relatively accurate numerical estimate of 2R=82 ?.     

超短电子脉冲在聚焦强激光场中的三维有质动力散射

采用三维测试粒子模拟(test-padicle simulation)的方法，研究了聚焦的飞秒脉冲强激光与超短电子束进行有质动力的散射过程.详细讨论了超短电子束经过不同激光延迟下有质动力散射的空间分布特征和能谱特征.提出了通过测量电子束的空间分布和能谱分布实现对超短脉冲电子束的脉冲宽度测量的方法. 关键词： 有质动力散射 聚焦的飞激光 飞秒电子束     

扇形多注强流相对论电子束的产生与传输研究

多注相对论速调管相对于常规相对论速调管, 每注电子束具有更低的导流系数和更低的空间电荷力, 却具有更高的束波转换效率. 本文基于这方面的需求, 通过三维软件模拟与实验研究了扇形多注强流相对论电子束的产生与传输. 通过建立电子枪的三维模型, 分析了阴极端面静电场的分布及其对电子束产生的影响; 通过粒子模拟获得了发射束流, 然后通过粒子跟踪仿真, 得到了电子束在空心漂移管和多扇形孔漂移管中传输的束斑图, 并对其进行了理论分析与解释. 模拟和实验结果表明, 电子束在空心漂移管传输过程中不仅绕束自身中心旋转, 还绕系统的中心旋转, 通过旋转多扇形孔漂移管实现对中的方法可提高传输效率.     

激光等离子体不稳定性的入射光强度效应

用3维粒子模拟程序研究了相对论强激光和高密度等离子体相互作用引起的电磁不稳定。数值模拟表明,在线偏振强激光作用下,等离子体表面出现了电磁不稳定性。形成的不稳定结构随时间发展和激光功率密度的增加进一步深入到等离子体内部,最终使等离子体表面处激发饱和自生磁场。这种由电子速度各向异性而产生的自生磁场对激光有质动力推开电子时所形成的电子热流产生抑制作用,并将直接影响电子加速效率。     

Elastic correlations in nucleosomal DNA structure

The structure of DNA in the nucleosome core particle is studied using an elastic model that incorporates anisotropy in the bending energetics and twist-bend coupling. Using the experimentally determined structure of nucleosomal DNA [T. J. Richmond and C. A. Davey, Nature (London) 423, 145 (2003)], it is shown that elastic correlations exist between twist, roll, tilt, and stretching of DNA, as well as the distance between phosphate groups. The twist-bend coupling term is shown to be able to capture these correlations to a large extent, and a fit to the experimental data yields a new estimate of G = 25 nm for the value of the twist-bend coupling constant.     

Particle dispersion on rapidly folding random heteropolymers

We investigate the dynamics of a particle moving randomly along a disordered heteropolymer subjected to rapid conformational changes which induce superdiffusive motion in chemical coordinates. We study the antagonistic interplay between the enhanced diffusion and the quenched disorder. The dispersion speed exhibits universal behavior independent of the folding statistics. On the other hand it is strongly affected by the structure of the disordered potential. The results may serve as a reference point for a number of translocation phenomena observed in biological cells, such as protein dynamics on DNA strands.     

Spectral methods for study of the G-protein-coupled receptor rhodopsin. II. Magnetic resonance methods

This article continues our review of spectroscopic studies of G-protein-coupled receptors. Magnetic resonance methods including electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) provide specific structural and dynamical data for the protein in conjunction with optical methods (vibrational, electronic spectroscopy) as discussed in the accompanying article. An additional advantage is the opportunity to explore the receptor proteins in the natural membrane lipid environment. Solid-state ²H and ¹³C NMR methods yield information about both the local structure and dynamics of the cofactor bound to the protein and its light-induced changes. Complementary site-directed spin-labeling studies monitor the structural alterations over larger distances and correspondingly longer time scales. A multiscale reaction mechanism describes how local changes of the retinal cofactor unlock the receptor to initiate large-scale conformational changes of rhodopsin. Activation of the G-protein-coupled receptor involves an ensemble of conformational substates within the rhodopsin manifold that characterize the dynamically active receptor.     

Electron Interference in a Double‐Dopant Potential Structure

Herein, an analysis of interference effects as a result of the electron evolution within a coherent transport medium is presented, offering a double‐dopant Coulomb potential structure. Injection of coherent electron states into the structure is used to investigate the effects on the current transport behavior within the quantum Wigner phase space picture. Quantum effects are outlined by using classical simulation results as a reference frame. The utilized signed particle approach inherently provides a seamless transition between the classical and quantum domain. Based on this the occurring quantum effects caused by the non‐locality of the action of the quantum potential, leading to spatial resonance, can be indentified. The resulting interference patterns enable novel applications in the area of entangletronics.     

DNA与组蛋白相互作用的布朗动力学研究

在真核生物中,DNA按左手手征性的方式,缠绕在组蛋白八聚体的周围,形成稳定的核小体结构.文章作者运用布朗动力学,数值模拟了DNA与组蛋白相互作用最终形成核小体的动力学过程,揭示了DNA与组蛋白相互作用的详细图景,并提出了组蛋白八聚体旋转模型,以解释这一过程.文章作者还计算了组成核小体的DNA在受到拉伸力时,组蛋白被从核小体中剥离下来的动力学过程,得到了组装和剥离过程的详细图像,给出了与前人单分子实验一致的拉伸力与拉伸长度的关系曲线和拉伸台阶.此外,还通过建立的组蛋白手征性模型,模拟了核小体手征性的形成过程,发现DNA的缠绕方向强烈依赖于组蛋白的手征性,显示出环境温度对核小体手征性有重要影响.     

Ka波段同轴多注相对论速调管的电子束引入

首先通过理论分析确定影响多注电子束引入效率的主要因素,确定初步的结构参数;其次利用三维粒子模拟软件建立Ka波段相对论多注二极管模型进行仿真优化,使电子束引入效率达到89%;并开展了电子束的产生与传输实验研究,验证了粒子模拟仿真结果。在电子束电压502 kV、束流4.34 kA、轴向磁感应强度0.76 T的条件下,电子束引入效率达到了72%,由电子束轰击尼龙靶材获得的电子束束斑图表明,电子束在产生与传输过程中形状未发生畸变,产生的电子束直径约为2 mm。模拟和实验研究验证了设计的强流多注二极管可以产生高品质的电子束和实现高效率的电子束引入。     

Reformed Solitary Kinetic Alfven Waves due to Dissipations and Auroral Electron Acceleration

The physical nature of the auroral electron acceleration has been an outstanding problem in space physics for decades. Some recent observations from the auroral orbit satellites, FREJA and FAST, showed that large amplitude solitary kinetic Alfvén waves (SKAWs) are a common electromagnetic active phenomenon in the auroral magnetosphere. In a low-β (i.e., β/2<<m_e/m_i<<1) plasma, the drift velocity of electrons relative to ions within SKAWs is much larger than thermal velocities of both electrons and ions. This leads to instabilities and causes dissipations of SKAWs. In the present work, based on the analogy of classical particle motion in a potential well, it is shown that a shock-like structure can be formed from SKAWs if dissipation effects are included. The reformed SKAWs with a shock-like structure have a local density jump and a net field-aligned electric potential drop of order of m_ev_A²/e over a characteristic width of several λ_e. As a consequence, the reformed SKAWs can efficiently accelerate electrons field-aligned to the order of the local Alfvén velocity. In particular, we argue that this electron acceleration mechanism by reformed SKAWs can play an important role in the auroral electron acceleration problem. The result shows that not only the location of acceleration regions predicted by this model is well consistent with the observed auroral electron acceleration region of 1—2 R_E above the auroral ionosphere, but also the accelerated electrons from this region can obtain an energy of several keV and carry a field-aligned current of several μA/m² which are comparable to the observations of auroral electrons.     

电子发现100年（续）

电子发现１００年（续）乔晓华孙玉霞（徽州师范专科学校物理系，安徽黄山２４５０２１）另一方面，塞曼在１８９６年发现钠火焰在磁铁的作用下，Ｄ线比通常变宽了，这就是所谓的“塞曼效应”．塞曼认为洛伦兹的电子论能够解释这一现象，便立即把实验结果告诉给洛伦兹．洛...     

基于开槽单矩形栅和圆形电子注的W波段返波振荡器

提出将开槽单矩形栅和圆形电子注作为 W波段返波振荡器的注波互作用回路. 使用 3维电磁场仿真软件CST-MWS对开槽单矩形栅的高频特性进行了仿真分析, 研究结果表明: 相对于传统单矩形栅, 新结构的基模带宽有所展宽; 基模与高次模发生模式竞争的可能性很小; 在采用圆形电子注时新结构能获得大得多的耦合阻抗; 新结构的趋肤损耗略有改善. 将该慢波结构应用于设计一支以94 GHz为频带中心的W 波段返波振荡器: 设计了简洁的慢波过渡部分、输出耦合器和终端匹配衰减器, 优化参数后获得了良好的信号传输特性; 利用粒子模拟软件CST-PS对返波振荡器模型进行了三维大信号注波互作用计算, 设定合适的电子注电流等参数后, 调整工作电压在较宽的频带内获得了瓦级的功率输出, 电子效率在整个频带范围内优于1%. 关键词： 开槽单矩形栅 圆形电子注 返波振荡器 W波段     

Collective modes and dynamic structure factor of a two-dimensional electron fluid

The electrons bound to the surface of liquid dielectrics by image forces are described as a two-dimensional, classical, one-component plasma with inverse distance interactions. Exact expressions for the collective modes and the dynamic structure factor are obtained from first principles in the limit of long wavelengths. The differences and analogies with uncharged particle fluids and with the three-dimensional one-component plasma are explicitly displayed. The previously used mean-field approximation is shown not to describe weakly coupled systems and to be inadequate in the long-wavelength region.     

Histone core slips along DNA and prefers positioning at the chain end

We studied the stability and dynamics of a model of a nucleosome, the fundamental unit for the packing of long DNA in eukaryotes, using a Brownian dynamics simulation. For the proper folding of a stiff polymer on a core particle, moderate attractive interaction is shown to be essentially important, which explains the empirical experimental protocol for the reconstitution of nucleosomes. The effect of the chain end on the positioning of the core particle is examined and compared with the experimental data by atomic force microscopy measurement. It is also suggested that the core particle exhibits sliding motion along the chain as a manifestation of Brownian motion.     

非晶化与量子限域效应

当材料尺度减小到几个纳米时，材料内部电子结构会表现为分立能级，这就是所谓的量子限域效应。通过晶态和非晶Pd纳米颗粒的单电子隧穿实验发现，在晶态Pd颗粒中能观察到量子限域效应，而在同样大小的非晶Pd颗粒中却没有观察到。考虑到有序／无序结构的静态效应并结合电子散射等动态效应，解释了非晶Pd颗粒实验中没有观察到量子限域效应的原因。这一结果表明，尺寸减小并不足以使纳米体系表现量子行为，原子结构有序度对于决定纳米体系表现经典行为或量子行为具有同等重要作用。     

染料敏化太阳电池内部光路折转对电子传输特性的影响

基于染料敏化太阳电池(DSC)光阳极的反射层结构,建立了含有 光路折转的电子连续性方程.计算和分析了不同吸收条件和反射条件下的调制光 电流频率响应特性,研究了DSC内部光路折转对电子传输特性的影响.通过不同膜 厚的强度调制光电流谱 测试表明, 建立的模型反映了DSC内部光路折转时调制光电流频率 响应.动力学研究结果表明, 在含有反射层的DSC中,电子传输动力学过程依赖 于光吸收系数、薄膜厚度以及大颗粒反射能力等因素. DSC内部光路折转导 致较深陷阱被电子填充,缩短了电子在陷阱中的停留时间, 减小了俘获/脱俘影响, 使电子传输过程加快.     

磁绝缘传输线阻抗变化对空间电子流的影响

介绍了磁绝缘传输线的阻抗大小、传输电压及电流大小对其产生的空间电子流大小及阴极电子鞘层厚度的影响。运用CHIPIC软件建立四层圆盘锥形磁绝缘传输线A层的近似化三维模型进行模拟,对比理论计算值与模拟值,并与相关文献的二维模拟结果进行对照,验证三维模拟的正确性。最后,模拟研究了圆盘锥形磁绝缘传输线阴阳极之间的间距变化对传输效率的影响。