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Yongxi Zhao Xiaolei Zuo Qian Li Feng Chen Yan-Ru Chen Jinqi Deng Da Han Changlong Hao Fujian Huang Yanyi Huang Guoliang Ke Hua Kuang Fan Li Jiang Li Min Li Na Li Zhenyu Lin Dingbin Liu Juewen Liu Libing Liu Xiaoguo Liu Chunhua Lu Fang Luo Xiuhai Mao Jiashu Sun Bo Tang Fei Wang Jianbin Wang Lihua Wang Shu Wang Lingling Wu Zai-Sheng Wu Fan Xia Chuanlai Xu Yang Yang Bi-Feng Yuan Quan Yuan Chao Zhang Zhi Zhu Chaoyong Yang Xiao-Bing Zhang Huanghao Yang Weihong Tan Chunhai Fan 《中国科学:化学(英文版)》2021,(2)
Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided. 相似文献
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Dr. Lajos Kovács 《Chemical record (New York, N.Y.)》2023,23(1):e202200203
Nucleic acids play a pivotal role in life processes. The endeavours to shed light on the essential properties of these intriguing building blocks led us to the synthesis of different analogues and the investigation of their properties. First various peptide nucleic acid monomers and oligomers have been synthesized, using an Fmoc/acyl protecting group strategy, and their properties studied. The serendipitous discovery of a side reaction of coupling agents led us to the elaboration of a peptide sequencing method. The capricious behaviour of guanine derivatives spurred the determination of their substitution pattern using 13C, 15N NMR, and mass spectrometric methods. The properties of guanines initiated the logical transition to the study of supramolecular systems composed of purine analogues. Thus, xanthine and uracil derivatives have been obtained and their supramolecular self-assembly properties scrutinized in gas, solid, and liquid states and at solid-liquid interfaces. 相似文献
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In biology, nucleic acids are carriers of molecular information: DNA's base sequence stores and imparts genetic instructions, while RNA's sequence plays the role of a messenger and a regulator of gene expression. As biopolymers, nucleic acids also have exciting physicochemical properties, which can be rationally influenced by the base sequence in myriad ways. Consequently, in recent years nucleic acids have also become important building blocks for bottom-up nanotechnology: as molecules for the self-assembly of molecular nanostructures and also as a material for building machinelike nanodevices. In this Review we will cover the most important developments in this growing field of nucleic acid nanodevices. We also provide an overview of the biochemical and biophysical background of this field and the major "historical" influences that shaped its development. Particular emphasis is laid on DNA molecular motors, molecular robotics, molecular information processing, and applications of nucleic acid nanodevices in biology. 相似文献
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Nadrian C. Seeman 《Angewandte Chemie (International ed. in English)》1998,37(23):3220-3238
Knots, polyhedra, and Borromean rings with specific structural and topological features can be made from DNA. Biotechnologists have been exploiting the programmability of DNA intermolecular associations for a quarter of a century. These operations have now been applied successfully to branched DNA species to produce complex target structures (for example, the cube shown in the picture) and a nanomechanical device. The assembly of two-dimensional crystals with programmed topographic characteristics demonstrates the simplicity of translating design into surface structures. 相似文献
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Dr. Da Han Dr. Huaizhi Kang Dr. Tao Zhang Cuichen Wu Dr. Cuisong Zhou Dr. Mingxu You Prof. Dr. Zhuo Chen Prof. Dr. Xiaobing Zhang Prof. Dr. Weihong Tan 《Chemistry (Weinheim an der Bergstrasse, Germany)》2014,20(20):5866-5873
Researchers increasingly visualize a significant role for artificial biochemical logical systems in biological engineering, much like digital logic circuits in electrical engineering. Those logical systems could be utilized as a type of servomechanism to control nanodevices in vitro, monitor chemical reactions in situ, or regulate gene expression in vivo. Nucleic acids (NA), as carriers of genetic information with well‐regulated and predictable structures, are promising materials for the design and engineering of biochemical circuits. A number of logical devices based on nucleic acids (NA) have been designed to handle various processes for technological or biotechnological purposes. This article focuses on the most recent and important developments in NA‐based logical devices and their evolution from in vitro, through cellular, even towards in vivo biological applications. 相似文献
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Sarah J. Reinholt Antje J. Baeumner 《Angewandte Chemie (International ed. in English)》2014,53(51):13988-14001
The detection of nucleic acids (NAs) within micro total analysis systems (μTASs) for point‐of‐care use is a rapidly developing research area. The efficient isolation of NAs from a raw sample is crucial for these systems to be maximally effective. The use of microfluidics assists in reducing sample sizes and reagent consumption, increases speed, avoids contamination, and enables automation. Through miniaturization into microchips, new techniques have been realized that would be unfavorable and inconvenient to use on a macroscopic scale, but provide an excellent platform for the purification of NAs on a microscopic scale. This Review considers the complexities of NA isolation with miniaturized and microfluidic devices, as well as the considerations when choosing a technique for microfluidic NA isolation, along with their advantages, disadvantages, and potential applications. The techniques presented include using silica‐based surfaces, functionalized paramagnetic beads, oligonucleotide‐modified polymer surfaces, pH‐dependent charged surfaces, Al2O3 membranes, and liquid‐phase isolation. This Review provides a basis to develop the chemistry to improve NA isolation and move it toward achieving 100 % efficiencies. 相似文献
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光敏感基团作为光化学开关被广泛应用于各种生物过程的光调控中。特别是过去十几年内,核苷酸、寡聚核苷酸和DNA/RNA的光敏修饰策略得到了长足的发展,并在细胞信号传导和靶基因的功能调控等诸多生物学研究中发挥重要的作用。本文主要针对常用的光敏感基团、光敏感核酸及其化学生物学研究进展进行简要综述,并对未来核酸光化学生物学的研究进行了展望。 相似文献
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Wengel J 《Organic & biomolecular chemistry》2004,2(3):277-280
Nucleic acids and analogues are suitable building blocks for reliable self-assembly of nanometer-sized two- or three-dimensional materials. In order to mimic or approach nature with respect to size and function, Angstrom-scale chemical engineering is emerging as pivotal for future developments. Efforts within nucleic acid nanotechnology will be focussed on generating rigid and stable low nanometer-sized structures carrying functionalities with predictable spatial positioning allowing, by encoded self-assembly, functional nucleic acid architectures to be built towards applications within the biological and material sciences. 相似文献
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Electrochemiluminescence (ECL) is a technique by which a chemiluminescent reaction is generated from reagents produced in the vicinity of an electrode surface when a potential is applied. ECL methods have more significiant advantages over more convential chemiluininescent techniques. In particular, the necessary reactants are produced in situ at a given electrode, enabling the reaction to be controlled through small changes in the applied potential. Furthermore, since light emission is located only in the immediate vicinity of the electrode surface, light colletion is not only efficient but facile. 相似文献
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Keiji Murayama Yoshihiro Tanaka Takasuke Toda Prof. Dr. Hiromu Kashida Prof. Hiroyuki Asanuma 《Chemistry (Weinheim an der Bergstrasse, Germany)》2013,19(42):14151-14158
The stabilities of duplexes formed by strands of novel artificial nucleic acids composed of acyclic threoninol nucleic acid (aTNA) and serinol nucleic acid (SNA) building blocks were compared with duplexes formed by the acyclic glycol nucleic acid (GNA), peptide nucleic acid (PNA), and native DNA and RNA. All acyclic nucleic acid homoduplexes examined in this study had significantly higher thermal stability than DNA and RNA duplexes. Melting temperatures of homoduplexes were in the order of aTNA>PNA≈GNA≥SNA?RNA>DNA. Thermodynamic analyses revealed that high stabilities of duplexes formed by aTNA and SNA were due to large enthalpy changes upon formation of duplexes compared with DNA and RNA duplexes. The higher stability of the aTNA homoduplex than the SNA duplex was attributed to the less flexible backbone due to the methyl group of D ‐threoninol on aTNA, which induced clockwise winding. Unlike aTNA, the more flexible SNA was able to cross‐hybridize with RNA and DNA. Similarly, the SNA/PNA heteroduplex was more stable than the aTNA/PNA duplex. A 15‐mer SNA/RNA was more stable than an RNA/DNA duplex of the same sequence. 相似文献