Simultaneous determination of galanthamine and lycorine in Lycoris radiata by a capillary electrophoresis with an electrochemiluminescence method |
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| Authors: | Yanchao Wang Guimei Zhu Xia Li Zaibin Hao |
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| Affiliation: | 1. College of Life Science, Northeast Agricultural University, , Harbin, China;2. College of Chemistry and Bioengineering, Guilin University of Technology, , Guilin, China |
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| Abstract: | A novel capillary electrophoresis with electrochemiluminescence determination method was developed for the determination of two alkaloids based on the electrochemiluminescence signal enhancement effect of the tertiary amine group on tris(2,2′‐bipyridyl)ruthenium(II). A linear relationship between the electrochemiluminescence peak area and concentrations of galanthamine and lycorine in the range of 0.07 ～ 17 μg/mL and 0.07 ～ 18 μg/mL was obtained and the detection limit was 0.008 and 0.002 μg/mL, respectively. The method is selective, simple, and convenient. It had been successfully applied to the analysis of galanthamine and lycorine in Lycoris radiata samples purchased from a local market. |
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| Keywords: | Capillary electrophoresis Electrochemiluminescence Galanthamine Lycorine |
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