| Mechanical Properties of Breast Cancer Cell Membrane Studied with Optical Tweezers |
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| 作者姓名： | 郭红莲 刘春香 段建发 隆雨强 韩学海 李兆霖 程丙英 张道中 |
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| 作者单位： | [1]OpticalPhysicsLaboratory,InstituteofPhysics,ChineseAcademyofSciences,Beijing100080 [2]StateKeyLaboratoryofBiomacromolecles,InstituteofBiophysics,ChineseAcademyofSciences,Beijing100101 |
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| 摘 要： | Membrane tethers are extracted from breast cancer cells using a force generated by an optical trap. It is experimentally obtained that the radius of tether is about 0.1μm and the static tether force is about 8.5 pN. Calculations based on the experimental measurements give a bending modulus for the tether of 1.35 x 10^-19 N.m and a surface membrane tension of 6.76 x 10^-6 N/m in the breast cancer cell. The treatment with cytochalasin D results in the decreasing bending modulus and decreasing apparent surface tension. When the membrane protein caveolin is over-expressed, similar cases occur in bending modulus and apparent surface tension. In addition, the viscous resistance coefficient of the membrane is calculated to be 1.15pN.s/μm according to the dynamic tether forces obtained under different pulling velocities.
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| 关 键 词： | 机械性质 乳癌 细胞膜 光学镊子 |
Mechanical Properties of Breast Cancer Cell Membrane Studied with Optical Tweezers |
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| GUO Hong-Lian,LIU Chun-Xiang,DUAN Jian-Fa,Jiang Yu-Qiang,HAN Xue-Hai,LI Zhao-Lin,CHENG Bing-Ying,ZHANG Dao-Zhong.Mechanical Properties of Breast Cancer Cell Membrane Studied with Optical Tweezers[J].Chinese Physics Letters,2004,21(12):2543-2546. |
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| Authors: | GUO Hong-Lian LIU Chun-Xiang DUAN Jian-Fa Jiang Yu-Qiang HAN Xue-Hai LI Zhao-Lin CHENG Bing-Ying ZHANG Dao-Zhong |
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| Affiliation: | Optical Physics Laboratory, Institute of Physics, Chinese Academy of Sciences, Beijing 100080
State Key Laboratory of Biomacromolecles, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 |
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| Abstract: | |
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| Keywords: | 81 70 Fy 87 15 La |
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