CdSe Quantum Dots Based Electrochemiluminescence Immunosensor with Simple Structure for Ultrasensitive Detection of Salbutamol |
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| Authors: | Jing Zhang Fudong Cai Anping Deng Jianguo Li |
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| Affiliation: | 1. College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, P.?R. China tel: +86?51265882195, fax: +86?51265882195 tel: +86?51265882362, fax: +86?51265882362;2. The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, Suzhou 215123, P.?R. China |
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| Abstract: | A rapid and ultrasensitive electrochemiluminescence (ECL) competitive immunoassay based on CdSe quantum dots (QDs) and the shorter chain as possible (cysteamine and glutaraldehyde) has been designed for the detection of salbutamol (SAL). Cysteamine and glutaraldehyde made coating antigen immobilize well on the gold electrode surface through the reaction between functional groups, which brought about the simplicity of the immunosensor to some extent. Transmission electron microscopy image, dynamic light scattering, photoluminescence, ultraviolet‐visible absorption and electrochemical impedance spectra were used to characterize the prepared CdSe QDs and the cysteamine/glutaraldehyde/Ovalbumin‐SAL/anti‐SAL‐QDs immunosensor. In the air‐saturated PBS buffer containing 0.1 M K2S2O8 and 0.1 M KCl (pH 9.0), a strong ECL emission of QDs can be observed which depended linearly on the logarithm of the salbutamol concentration with a wide range from 0.05 ng mL?1 to 100 ng mL?1, and a detection limit of 0.0056 ng mL?1. The sensitivity, repeatability, and specificity of the ECL immunosensor have been evaluated. The sensor has been applied to real samples with satisfactory results. This work will open new ways of detecting food additive residue based on QDs ECL in immunoassays. |
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| Keywords: | CdSe quantum dots Competitive immunoassay Electrochemiluminescence Immunosensor Salbutamol |
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