Abstract:Using whole blood samples as raw materials, the wet digestion-atomic fluorescence method was used to determine the selenium content in blood. The blood sample is digested with nitric acid-perchloric acid, the selenium is reduced to hydrogen selenide with sodium borohydride, and the hydrogen selenide is loaded into the atomizer by argon. The fluorescence intensity of the generated atoms is within a certain range with the content of selenium in the test solution. It is proportional and quantified by external standard method. Use the digestion efficiency as an indicator to optimize the digestion conditions of the sample,the determination of selenium in blood is linear in the range of 0 -10 μg/L, the correlation coefficient is 0.9992, the minimum detection limit is 0.143μg/L, the relative standard deviation is 1.51% - 1.58%, and the average recovery rate is 90.86% - 104.62%. The atomic fluorescence determination method of selenium in blood has high sensitivity, good precision and stability, and can be applied to biological monitoring of selenium in blood.