| 烟草中9种有机酸的梯度离子色谱法测定研究 |
| 点此下载全文 |
|
|
| 中文摘要:采用淋洗液自动发生－梯度离子色谱分离，电导检测法分离测定了烟草中的甲酸、乙酸、乳酸、丙酸、丁酸、苹果酸、丙二酸、草酸和柠檬酸，并研究了9种有机酸在阴离子交换色谱中的保留行为。以淋洗液自动发生器生成的KOH为淋洗液，样品经提取、过滤稀释后，在IonPac AS11-HC阴离子交换色谱柱上分离，抑制电导检测器进行检测，一次进样可同时分离烟草中的常见有机酸。9种有机酸在优化条件下线性良好，相关系数r≥0.998 6，检出限（S/N=3）为0.013~0.065 μg/L，相对标准偏差（RSD）为0.7%~3.0%，加标回收率为93%~103%。方法简单、准确、灵敏度高，能够满足实际样品的测试要求。 |
| 中文关键词:离子色谱 淋洗液自动发生 测试 烟草 有机酸 |
| |
| Determination of Nine Organic Acids in Tobacco by Gradient Ion Chromatography |
|
|
| Abstract:A direct,simple and sensitive method was developed for the simultaneous separation and detection of nine organic acids,including lactate,acetate,propionate,formate,butyrate,malate,malonate,oxalate and citrate,in tobacco by anion exchange chromatography with eluent autogenerator and suppressed conductivity detection.The retention behaviors of organic acids on the anion exchange column were investigated using potassium hydroxide produced by EGC-ⅡKOH eluent autogenerator as eluent and the optimized gradient elution condition was obtained.The samples were prepared through extraction,filtration and dilution.The separation was performed on a Dionex IonPac AS11-HC anion exchange column.Under the optimized conditions,the calibration curves of all the analytes had good linearity.The detection limits ranged from 0.013 μg/L to 0.065 μg/L,and the relative standard deviations（RSDs） were in the range of 0.7%-3.0%.The spiked recoveries of nine organic acids were in the range of 93%-103%.The method was simple,accurate and sensitive，and could meet the actual requirements for the analysis of tobacco samples. |
| Key Words:ion chromatography eluent autogeneration detection tobacco organic acids |
| 引用本文：胡静，赵瑞峰，施文庄，李峰，沈光林.烟草中9种有机酸的梯度离子色谱法测定研究[J].分析测试学报,2011,30(10):1171-1174. |
| 摘要点击次数: 2725 |
| 全文下载次数: 1255 |
| 查看全文 下载PDF阅读器 |
|
|
|
|
|
