| 高效液相色谱法同时测定香兰素与邻位香兰素 |
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| 基金项目:辽宁省自然基金资助项目（20090655），辽宁省教育厅2008年度科研计划项目（2008S184） |
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| 中文摘要:建立了同时测定香兰素和邻位香兰素的高效液相色谱法。考察了流动相组成、柱温等因素对分析效率的影响。在流动相为5% 乙酸-乙腈（60∶40，体积比），流速10 mL/min，柱温25 ℃的优化条件下，香兰素和邻位香兰素可在5 min内实现分离。测定结果表明，香兰素和邻位香兰素在10~240 mg/L范围内线性关系良好，相关系数均为0999 7；方法的检出限（S/N=3）分别为006、002 mg/L；样品的加标回收率为99%~102%，相对标准偏差为02%~06%。该方法操作简单、快速，用于实际样品的分析，结果满意。 |
| 中文关键词:高效液相色谱法 香兰素 邻位香兰素 同分异构体 |
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| Simultaneous Determination of Vanillin and o Vanillin by High Performance Liquid Chromatography |
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| Abstract:A high performance liquid chromatographic method was developed for the simultaneous separation and determination of vanillin and o vanillin. Influences of mobile phase composition and column temperature on the separation were investigated. Under the condition of 5% acetic acid-acetonitrile （60∶40,by volume） as mobile phase at a flow rate of 10 mL/min and 25 ℃,the vanillin and o vanillin were separated in less than 5 min. The calibration curves of vanillin and o vanillin showed good linearities in the range of 10-240 mg/L with both correlation coefficients of 0999 7. The limits of detection（S/N=3） for vanillin and o vanillin were 006 mg/L and 002 mg/L,respectively. The average recoveries at three spiked levels were in the range of 99% -102% with relative standard deviations of 02% - 06%. The method was successfully used for the determination of vanillin and o vanillin in real samples with satisfactory results. |
| Key Words:high performance liquid chromatography vanillin o vanillin isomers |
| 引用本文：金海涛，马健瑜，王晓珣，陈星,任丽艳，关瑾，李云.高效液相色谱法同时测定香兰素与邻位香兰素[J].分析测试学报,2011,30(2):222-224. |
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