| 石墨烯-金纳米复合材料:水热合成及在生物传感器中的应用 |
| Graphene-Au Nanocomposite: Hydrothermal Synthesis and Application to Biosensor |
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| 摘要: 通过水热法合成了石墨烯-金纳米复合材料。透射电子显微镜直接证明了制备的石墨烯薄而透明的片状结构。X-射线光电子能谱和X-射线衍射结果也表明了金的存在和氧化石墨烯的还原。作为一个模型,将肌红蛋白固定到该复合材料修饰的玻碳电极上并用于生物传感器。所固定的肌红蛋白显示了一对对称的氧化还原峰并对过氧化氢的还原具有高的催化活性。在信噪比等于3的时侯该生物传感器的线性范围在0.1到1.5 μmol·L-1,检测限为0.05 μmol·L-1,并且具有好的选择性、重现性和稳定性。 |
| 关键词: 石墨烯 金 纳米复合材料 生物传感器 |
| 基金项目: 国家自然科学基金(No.20905038,20903057,21005040),“973”项目(No.2009CB930600,2012CB933301)资助项目。 |
| Abstract: The graphene-Au nanocomposites were synthesized through hydrothermal route. Transmission electron microscopy (TEM) images gave direct evidence of the thin and transparent sheet structure of graphene. X-ray photoelectron spectroscopic (XPS) and X-ray diffraction (XRD) results also showed the presence of Au and the reduction of graphene oxide. As a model, myoglobin (Mb) was entrapped onto the nanocomposite modified glassy carbon electrode (GCE) and applied to construct a sensor. The immobilized Mb showed a pair of well-defined redox peaks and high catalytic activity for the reduction of H2O2. The biosensor with a linear range from 0.1 to 1.5 μmol·L-1 and a detection limit of 0.05 μmol·L-1 (S/N=3) showed good selectivity, reproducibility and stability. The graphene-Au nanocomposites provide a novel matrix for protein immobilization and the construction of biosensors via the direct electron transfer of protein. |
| Keywords: graphene gold nanocomposite biosensor |
| 投稿时间：2012-09-22 修订日期：2012-12-31 |
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| 冯晓苗,闫真真.石墨烯-金纳米复合材料:水热合成及在生物传感器中的应用[J].无机化学学报,2013,29(5):1051-1056. |
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