诃子抗嗜水气单胞菌活性组分分离及其对鲫鱼的毒性试验

研究以抑菌圈直径大小作为评价指标，利用提取分离技术对诃子进行抗嗜水气单胞茵活性成分分离追踪，并对诃子的抗菌活性单体进行安全评价．结果显示，诃子的抗嗜水气单胞茵的活性部位为乙酸乙酯萃取部位，其浓度为4mg·L^-1时，抑茵圈直径为18mm．活性部位经多次柱层析分离，得到一白色针状晶体．抑菌试验结果显示，最小抑菌浓度（MIC）和最小杀菌浓度（MBC）分别为2．8mg-L^-1和5．6mg·L^-1．急性毒性实验结果显示，活性单体对鲫鱼的96h半致死浓度为220．7mg·L^-1，其安全浓度为85．2mg·L^-1，但其化学结构还有待于进一步研究．

Isolation of Active Component from Terminalia chebula Against Ameromonas hydrophila and Acute Toxicity Experiment on Carassius auratus

The present study is intended to investigate the antibacterial activity of crude extracts and pure compound from the fruits of Terminalia chebula. The ethyl acetate extract shows a promising antibacterial activity against Ameromonas hydrophila （when the concentrations is set for 4 mg.L^-1, diameter of inhibition zone is 18 mm）. Based on these findings, the ethyl acetate extract is fractionated on silica gel column chromatography in a bioassay-guided fractionation which produces an active compound （white crystal） showing potent activity. The active compound exhibit significant activity against Ameromonas hydrophila with minimal inhibitory concentrations （MIC）. The minimal bactericidal concentrations （MBC） in the process afore are regulated for 2.8 mg.L^-1 and 5.6 mg.L^-1, respectively. The acute toxicities （LCs0） of the active compound for Carassius auratus is found to be 220.7 mg-L^-1, the safety concentration 85.2 mg·L^-1. The structure is not fully explored at this point and the research on it will be further embarked on.