Enhancement of electroosmotic flow using zwitterionic additives |
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Authors: | MacDonald Amy M Sheppard Mary A W Lucy Charles A |
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Affiliation: | Department of Chemistry, University of Alberta, Gunning/Lemieux Chemistry Centre, Edmonton, Alberta, Canada. |
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Abstract: | Zwitterionic additives provide a means of altering the EOF without increasing conductivity. The magnitude of the EOF in a bare silica capillary increased by as much as 69% upon addition of 500 mM of zwitterion to the running buffer. The EOF enhancement increases linearly with the zwitterion concentration. With zwitterionic additives of the form +NH3-(CH2)n-COO-, the magnitude of the EOF increase is directly related to the number of methylene groups, (n), which ranges from n = 1 to 7. The endgroups on the zwitterions also affect the EOF enhancement. The effect of Z1-methyl (+N(CH3)3CH2CH2CH2SO3-) on EOF was not a function of either the buffer cation or pH. The EOF enhancement is a function of the dielectric increment of the additive and the nature of the amine functionality. |
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Keywords: | Aminocarboxylic acid Capillary electrophoresis EOF Z1‐methyl Zwitterion |
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