| CHO-EPO-EGFR细胞株的构建及在光固定EGF材料上的生长 |
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| 引用本文： | 关燕清,郭宝江. CHO-EPO-EGFR细胞株的构建及在光固定EGF材料上的生长[J]. 离子交换与吸附, 2000, 16(3): 271-274 |
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| 作者姓名： | 关燕清 郭宝江 |
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| 作者单位： | 华南师范大学生物技术研究所,广州市,510631 |
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| 基金项目： | 广东省自然科学（青年）基金 |
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| 摘 要： | 采用人工细胞（ｌｉｐｏｓｏｍｅ）法将两种质粒ｐＣＥＰＯ及ｐＲＣ／ＣＭＶ／ＥＧＦＲ转染宿主细胞－中国仓鼠卵巢细胞（ＣＨＯ），得到表达水平稳定，形态良好的细胞株ＣＨＯ－ＥＰＯ－ＥＧＦＲ，研究该细胞株在光固定ＥＧＦ的聚苯乙烯材料上生长情况。
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| 关 键 词： | 细胞生长活性 CHO－EPO－EGFR细胞株 光固定EGF |
The construction of CHO-EPO-EGFR cells and Cells growth on the photo-immobilization of Epidermal Growth Factor polystyrene plate |
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| GUAN Yanqing,GUO Baojiang. The construction of CHO-EPO-EGFR cells and Cells growth on the photo-immobilization of Epidermal Growth Factor polystyrene plate[J]. Ion Exchange and Adsorption, 2000, 16(3): 271-274 |
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| Authors: | GUAN Yanqing GUO Baojiang |
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| Affiliation: | Biotechnology Research Institute. South China Normal University Guangzhou 510631 |
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| Abstract: | The liposome method was used to transfect two plasmid pCEPO and pRC/CMV/EGFR to Chinese Hamster Ovary cell (CHO-K1). A cell line (CHO_EPO-EGFR) with high level and stable expression was obtained. The cells were cultured under the serum-free conditions, and cells growth activity was investigated compared with CHO-K1. |
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| Keywords: | Liposome EPO Chinese hamster ovary cells (CHO-K1) Epidermal growth factor receptor (EGFR) Cells growth activity |
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