丝印电极法体外筛选黄嘌呤氧化酶抑制剂方法研究

建立丝印电极快速筛选黄嘌呤氧化酶(XO)抑制剂的新方法. 利用丝网印刷技术制作一次性使用的丝印碳糊电极, 采用吸附法将羧基化的多壁碳纳米管(MWNTs)修饰在电极表面, 建立测定尿酸(UA)的简单快捷的计时电流分析方法, 将该法应用于XO抑制剂的体外筛选. 在0.3 V (vs. Ag/AgCl)的工作电位下, UA在MWNTs修饰的丝印电极上产生灵敏的响应电流, 响应时间30 s, 测定UA的线性范围为2～300 μmol•L^－1, 线性方程为Y (μmol•L^－1)＝43.8240X (μA)－0.1592, r＝0.9998, 最低检出限为1 μmol•L^－1. 用该法对4种中药水提物进行了筛选, 相对空白对照组, 桑寄生500 μg•mL^－1浓度组有显著性差异. 该法简单、快速, 电极制作成本低, 所需仪器简单, 适用于体外大量筛选XO抑制剂.

Screening Method for Xanthine Oxidase Inhibitor Screen Printed Electrode

To establish a new screening method for xanthine oxidase inhibitor using screen printed electrode the one-off screen printed carbon paste electrode was prepared using screen printing technology. Carboxylic multi-walled carbon nanotubes were absorbed on the working surface of the screen printed electrode. This is a simple and rapid amperometry analysis method of uric acid determination which was applied to xanthine oxidase inhibitor screening in vitro. A sensitive response was obtained at an applied potential of 0.3 V vs. Ag/AgCl. The response time is 30 s. A linear response to uric acid was obtained up to 300 μmol• L^－1 with a limit of detection of 1 μmol•L^－1. The linear regression equation is Y＝43.8240X－0.1592 (r＝0.9998). The analysis was applied to four kinds of water extracts of traditional Chinese medicine. The group of Laranthus tanakae with a concentration of 500 μg•mL^－1 is significantly different from the control group. The method is simple and rapid with low manufacturing costs of the electrode and a simple apparatus.