| 高效液相色谱法同时测定苏木中的巴西苏木素和原苏木素B |
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| 引用本文： | 陈雪敏,胡永钢,李美萍,冯彦琳,张生万.高效液相色谱法同时测定苏木中的巴西苏木素和原苏木素B[J].分析试验室,2012(4):98-101. |
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| 作者姓名： | 陈雪敏 胡永钢 李美萍 冯彦琳 张生万 |
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| 作者单位： | 山西大学化学化工学院;山西大学生命科学学院 |
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| 摘 要： | 建立了同时测定苏木中巴西苏木素和原苏木素B的高效液相色谱检测方法。采用Agela Venusil XBP-C18(4.6 mm×150 mm,5μm)色谱柱,以甲醇-0.2%甲酸溶液为流动相,梯度洗脱,流速1.0 mL/min,测定温度为35℃,检测波长285 nm。巴西苏木素和原苏木素B的质量浓度均在0.005～1.0 mg/mL时与色谱峰面积之间线性关系良好,相关系数都为0.9999;回收率范围分别为96.7%～101.4%和99.4%～103.0%,相对标准偏差(RSD)为2.0%和1.3%。该法可以用于苏木药材的鉴定。
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| 关 键 词： | 高效液相色谱 苏木 巴西苏木素 原苏木素B |
Determination of brazilin and protosappanin B in sappan lignum by high performance liquid chromatography |
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| CHEN Xue-min,HU Yong-gang,LI Mei-ping,FENG Yan-lin,and ZHANG Sheng-wan.Determination of brazilin and protosappanin B in sappan lignum by high performance liquid chromatography[J].Chinese Journal of Analysis Laboratory,2012(4):98-101. |
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| Authors: | CHEN Xue-min HU Yong-gang LI Mei-ping FENG Yan-lin and ZHANG Sheng-wan |
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| Institution: | 1(1.School of Life Science,Shanxi University,Taiyuan 030006;2.School of Chemistry and chemical Engineering,Shanxi University,Taiyuan 030006) |
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| Abstract: | A high performance liquid chromatographic(HPLC)method was developed for the determination of brazilin and protosappanin B in Sappan Lignum.The HPLC separation was performed on a Agela Venusil XBP-C18 column with a gradient elution program of methanol-0.2% methanoic acid solution as the mobile phase at a flow rate of 1.0 mL/min and 35℃.The detection wavelength was 285 nm.There was good linear relationship between the mass concentrations and the peak areas of brazilin and protosappanin B in the range of 0.005-1.0 mg/mL.The recoveries were 96.7%~101.4% and 99.4%~103.0% with the relative standard deviations of 2.0% and 1.3%,respectively.The method not only can be used in quality control of Sappan Lignum. |
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| Keywords: | High performance liquid chromatography Sappan Lignum Brazilin Protosappanin B |
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