Separation of ADAM derivatives of carnitine and acyl-carnitines by capillary electrophoresis |
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Authors: | S Kiessig C Vogt and G Werner |
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Institution: | (1) University of Leipzig, Department of Chemistry and Mineralogy, Institute of Analytical Chemistry, Linnéstrasse 3, D-04103 Leipzig, Germany, DE |
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Abstract: | The separation of carnitine, acetylcarnitine and palmitoylcarnitine as ADAM (9-anthryldiazo-methane) derivatives was performed
using capillary electrophoresis. A buffer system with 90% methanol and various amounts of phosphoric acid and micelle forming
SDS was optimized with respect to the best resolution of the carnitine derivatives. A detection limit of 10 μmol/l or 32 ng
carnitine was determined by laser induced fluorescence detection. Under optimized conditions low carnitine contents in acylcarnitine
standards have been determined.
Received: 30 May 1996/Accepted: 17 June 1996 |
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