5-Hydroxymethylcytosine-selective oxidation with peroxotungstate |
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| Authors: | Okamoto Akimitsu Sugizaki Kaori Nakamura Akiko Yanagisawa Hiroyuki Ikeda Shuji |
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| Institution: | Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan. aki-okamoto@riken.jp |
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| Abstract: | Tungsten oxidation worked as a simple chemical reaction for the effective detection of 5-hydroxymethylcytosine in DNA, distinguishing it from its epigenetic precursors, 5-methylcytosine and unmethylated cytosine. The tungsten-oxidation product obtained from 5-hydroxymethylcytosine was trihydroxylated thymine and was detected as a cleavage band in gel electrophoresis after treatment with hot piperidine. |
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