Human prostate specific antigen (hPSA) purification and establishment of hPSA radioimmunoassay |
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Authors: | Weiqiang Zhong Li Chen Renzhi Wang |
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Affiliation: | (1) Institute of Radiation Medicine, 100850 Beijing, P.R. China |
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Abstract: | Human prostate specific antigen (hPSA) RIA was developed with hPSA and anti-PSA prepared in our laboratory. Its standard curve was linear with a sensitivity of 0.5 g/L. Serum PSA levels of 130 normal males ranged from 0 to 3.5 g/L (1.15±0.93 g/L), which are consistent with the results of other conventional RIA. The recovery, intra- and inter-assay coefficients of variation conform to the demands of RIA, and the results of 41 samples obtained by both the PSA RIA and PSA RIA of DPC were well correlated (=0.990). PSA level of 23 patients with prostatic carcinoma was 10400 g/L. |
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