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| 协同催化-溶剂萃取光度法测定血清中的唾液酸 | |
| 引用本文： | 邓健,袁亚莉,许金生,杨祚升,杨良勇. 协同催化-溶剂萃取光度法测定血清中的唾液酸[J]. 化学分析计量, 2001, 10(1): 9-10 |
| 作者姓名： | 邓健 袁亚莉 许金生 杨祚升 杨良勇 |
| 作者单位： | 1. 衡阳医学院化学教研室,421001 2. 衡阳师范学院,421008 3. 衡阳医学院第一附属医院 4. 衡阳市中心医院,421001 |
| 摘 要： | 利用Cu2+和Cr3+的协同催化作用,促进间苯二酚与唾液酸的显色,再用乙酸丁酯-正丁醇萃取有色物质,变换波长至625nm处测定血清中的唾液酸含量.建立了血清中唾液酸测定的新方法.方法的线性范围为0～1000mg/L,检出限为2.1 mg/L,相对标准偏差为1.59%～2.11%,回收率为96.2%～101.2%,糖类及抗坏血酸等物质对测定的干扰＜2%.该法简便、快速、灵敏、准确,可用于临床体液中唾液酸的测定. |
| 关 键 词： | 唾液酸 分光光度法 协同催化 萃取 血清 |
| 修稿时间： | 2000-09-25 |
DETERMINATION OF SIALIC ACID IN THE SERUM BY COORDINATE CATALYSIS-SOLVENT EXTRACTION SPECTROPHOTOMETRY |
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| Deng Jian,Yuan Yali Xu Jinsheng. DETERMINATION OF SIALIC ACID IN THE SERUM BY COORDINATE CATALYSIS-SOLVENT EXTRACTION SPECTROPHOTOMETRY[J]. Chemical Analysis And Meterage, 2001, 10(1): 9-10 | |
| Authors: | Deng Jian Yuan Yali Xu Jinsheng |
| Abstract: | The color reaction between sialic acid and 1,3-dihydroxy-benzene was promoted by coordinate-catalytic effect of Cu~(2+) and Cr~(3+) . The produced compound was extracted by butyl acetate-butanol, then the sialic acid in the serum was detected by spectrophotometry at 625 nm. So, a new method of determing the sialic acid in the serum was set up. The linear range of the method is 0 - 1000 mg/L, the detection limit is 2.1 mg/L, the relative standard deviation is 1.59% -2.11% and the recovery is 96.2% - 101.2%,the interference of sugar and ascorbic acid was less than 2 percent. This method is simple, rapid, sensitive and accurate and can be used in clinical assay for the sialic acid in the serum. |
| Keywords: | sialic acid spectrophotometry coordinate catalysis extraction serium |
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