Raman spectral properties of squamous cell carcinoma of oral tissues and cells |
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Authors: | L Su Y F Sun Y Chen P Chen A G Shen X H Wang J Jia Y F Zhao X D Zhou J M Hu |
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Institution: | 1.Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences,Wuhan University,Wuhan,China;2.The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology,Wuhan University,Wuhan,China |
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Abstract: | Early diagnosis is the key of the improved survival rates of oral cancer. Raman spectroscopy is sensitive to the early changes
of molecular composition and structure that occur in benign lesion during carcinogenesis. In this study, in situ Raman analysis
provided distinct spectra that can be used to discriminate between normal and malignant tissues, as well as normal and cancer
cells. The biochemical variations between different groups were analyzed by the characteristic bands by comparing the normalized
mean spectra. Spectral profiles of normal, malignant conditions show pronounced differences between one another, and multiple
Raman markers associated with DNA and protein vibrational modes have been identified that exhibit excellent discrimination
power for cancer sample identification. Statistical analyses of the Raman data and classification using principal component
analysis (PCA) are shown to be effective for the Raman spectral diagnosis of oral mucosal diseases. The results indicate that
the biomolecular differences between normal and malignant conditions are more obviously at the cellular level. This technique
could provide a research foundation for the Raman spectral diagnosis of oral mucosal diseases. |
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