Direct multicomponent analysis of beer samples constituents using micellar electrokinetic capillary chromatography |
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Authors: | Cortacero-Ramírez Sonia Segura-Carretero Antonio Cruces-Blanco Carmen Hernáinz-Bermúdez de Castro Miguel Fernández-Gutiérrez Alberto |
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Affiliation: | Research Laboratories of Grupo Cervezas Alhambra S.L., Faculty of Sciences, University of Granada, Spain. |
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Abstract: | A capillary electrophoretic method was developed using micellar electrokinetic capillary chromatography (MEKC) with diode-array detection to analyze simultaneously 26 beer constituents in a single procedure, including alcohols, iso-alpha-acids, amino acids, flavonoids, isoflavonoids, a vitamin, purine and pyrimidine bases. After filtration, sample components were separated with an uncoated capillary and a 25 mM sodium borate and 110 mM SDS buffer at pH 10.5. Analyses were run at 14 kV and 8 s of hydrodynamic injection with UV detection at 210 nm and 270 nm. The proposed method was successfully applied to the direct determination of beer constituents without any sample cleanup procedures. |
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Keywords: | Beer analysis Micellar electrokinetic capillary chromatography Multicomponent analysis |
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